Human Gene IRS2 (ENST00000375856.5_7) from GENCODE V47lift37
  Description: insulin receptor substrate 2 (from RefSeq NM_003749.3)
Gencode Transcript: ENST00000375856.5_7
Gencode Gene: ENSG00000185950.9_9
Transcript (Including UTRs)
   Position: hg19 chr13:110,405,042-110,438,930 Size: 33,889 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr13:110,408,651-110,438,400 Size: 29,750 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr13:110,405,042-110,438,930)mRNA (may differ from genome)Protein (1338 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: IRS2_HUMAN
DESCRIPTION: RecName: Full=Insulin receptor substrate 2; Short=IRS-2;
FUNCTION: May mediate the control of various cellular processes by insulin.
SUBUNIT: Interacts with PHIP (By similarity).
INTERACTION: Q96EB6:SIRT1; NbExp=2; IntAct=EBI-1049582, EBI-1802965;
SUBCELLULAR LOCATION: Cytoplasm, cytosol (By similarity).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Contains 1 IRS-type PTB domain.
SIMILARITY: Contains 1 PH domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: IRS2
Diseases sorted by gene-association score: diabetes mellitus, noninsulin-dependent* (117), fatty liver disease (8), gestational diabetes (6), obesity (2), insulin-like growth factor i (2), glucose metabolism disease (1), retinitis pigmentosa (0)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 23.72 RPKM in Cells - Cultured fibroblasts
Total median expression: 532.46 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -332.10530-0.627 Picture PostScript Text
3' UTR -960.703609-0.266 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002404 - Insln_rcpt_S1
IPR011993 - PH_like_dom
IPR001849 - Pleckstrin_homology

Pfam Domains:
PF00169 - PH domain
PF02174 - PTB domain (IRS-1 type)

SCOP Domains:
50729 - PH domain-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3FQW - X-ray MuPIT 3FQX - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9Y4H2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004871 signal transducer activity
GO:0005088 Ras guanyl-nucleotide exchange factor activity
GO:0005158 insulin receptor binding
GO:0005515 protein binding
GO:0016303 1-phosphatidylinositol-3-kinase activity
GO:0019901 protein kinase binding
GO:0019903 protein phosphatase binding
GO:0019904 protein domain specific binding
GO:0043548 phosphatidylinositol 3-kinase binding
GO:0046934 phosphatidylinositol-4,5-bisphosphate 3-kinase activity
GO:0071889 14-3-3 protein binding

Biological Process:
GO:0000165 MAPK cascade
GO:0002053 positive regulation of mesenchymal cell proliferation
GO:0002903 negative regulation of B cell apoptotic process
GO:0006006 glucose metabolic process
GO:0007165 signal transduction
GO:0007411 axon guidance
GO:0007420 brain development
GO:0008283 cell proliferation
GO:0008284 positive regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0009749 response to glucose
GO:0010748 negative regulation of plasma membrane long-chain fatty acid transport
GO:0010907 positive regulation of glucose metabolic process
GO:0019216 regulation of lipid metabolic process
GO:0030335 positive regulation of cell migration
GO:0030879 mammary gland development
GO:0030890 positive regulation of B cell proliferation
GO:0032000 positive regulation of fatty acid beta-oxidation
GO:0032024 positive regulation of insulin secretion
GO:0032869 cellular response to insulin stimulus
GO:0033673 negative regulation of kinase activity
GO:0036092 phosphatidylinositol-3-phosphate biosynthetic process
GO:0038111 interleukin-7-mediated signaling pathway
GO:0045725 positive regulation of glycogen biosynthetic process
GO:0046326 positive regulation of glucose import
GO:0046854 phosphatidylinositol phosphorylation
GO:0048015 phosphatidylinositol-mediated signaling
GO:0051897 positive regulation of protein kinase B signaling
GO:0055088 lipid homeostasis
GO:0071333 cellular response to glucose stimulus
GO:1901653 cellular response to peptide

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  JB406696 - Sequence 22 from Patent WO2012072685.
BC063516 - Homo sapiens cDNA clone IMAGE:4213553, partial cds.
AF073310 - Homo sapiens insulin receptor substrate-2 (IRS2) mRNA, complete cds.
AF161416 - Homo sapiens HSPC298 mRNA, partial cds.
BC110638 - Homo sapiens cDNA clone IMAGE:6157910.
BC042858 - Homo sapiens insulin receptor substrate 2, mRNA (cDNA clone IMAGE:5303945).
JD516667 - Sequence 497691 from Patent EP1572962.
JD552550 - Sequence 533574 from Patent EP1572962.
JD475514 - Sequence 456538 from Patent EP1572962.
JD302346 - Sequence 283370 from Patent EP1572962.
JD333408 - Sequence 314432 from Patent EP1572962.
JD439847 - Sequence 420871 from Patent EP1572962.
JD349314 - Sequence 330338 from Patent EP1572962.
BC012414 - Homo sapiens insulin receptor substrate 2, mRNA (cDNA clone IMAGE:3860131), partial cds.
BC066356 - Homo sapiens cDNA clone IMAGE:5288004.
BC026044 - Homo sapiens cDNA clone IMAGE:3928792.
JD553066 - Sequence 534090 from Patent EP1572962.
JD171750 - Sequence 152774 from Patent EP1572962.
JD228799 - Sequence 209823 from Patent EP1572962.
JD486650 - Sequence 467674 from Patent EP1572962.
JD544749 - Sequence 525773 from Patent EP1572962.
JD315682 - Sequence 296706 from Patent EP1572962.
JD091689 - Sequence 72713 from Patent EP1572962.
JD298821 - Sequence 279845 from Patent EP1572962.
JD315620 - Sequence 296644 from Patent EP1572962.
JD285152 - Sequence 266176 from Patent EP1572962.
JD563021 - Sequence 544045 from Patent EP1572962.
JD092701 - Sequence 73725 from Patent EP1572962.
JD492187 - Sequence 473211 from Patent EP1572962.
LF371075 - JP 2014500723-A/178578: Polycomb-Associated Non-Coding RNAs.
LF208025 - JP 2014500723-A/15528: Polycomb-Associated Non-Coding RNAs.
LF371076 - JP 2014500723-A/178579: Polycomb-Associated Non-Coding RNAs.
DL492002 - Novel nucleic acids.
DL490554 - Novel nucleic acids.
LF371077 - JP 2014500723-A/178580: Polycomb-Associated Non-Coding RNAs.
LF371078 - JP 2014500723-A/178581: Polycomb-Associated Non-Coding RNAs.
LF371079 - JP 2014500723-A/178582: Polycomb-Associated Non-Coding RNAs.
LF371080 - JP 2014500723-A/178583: Polycomb-Associated Non-Coding RNAs.
JD458518 - Sequence 439542 from Patent EP1572962.
JD458517 - Sequence 439541 from Patent EP1572962.
JD056229 - Sequence 37253 from Patent EP1572962.
JD271291 - Sequence 252315 from Patent EP1572962.
JD458510 - Sequence 439534 from Patent EP1572962.
JD406055 - Sequence 387079 from Patent EP1572962.
JD271292 - Sequence 252316 from Patent EP1572962.
JD458514 - Sequence 439538 from Patent EP1572962.
JD057274 - Sequence 38298 from Patent EP1572962.
JD406801 - Sequence 387825 from Patent EP1572962.
JD461601 - Sequence 442625 from Patent EP1572962.
JD056228 - Sequence 37252 from Patent EP1572962.
JD458513 - Sequence 439537 from Patent EP1572962.
JD458511 - Sequence 439535 from Patent EP1572962.
JD458512 - Sequence 439536 from Patent EP1572962.
LF371081 - JP 2014500723-A/178584: Polycomb-Associated Non-Coding RNAs.
LF371082 - JP 2014500723-A/178585: Polycomb-Associated Non-Coding RNAs.
JD127187 - Sequence 108211 from Patent EP1572962.
JD389986 - Sequence 371010 from Patent EP1572962.
JD391695 - Sequence 372719 from Patent EP1572962.
JD442302 - Sequence 423326 from Patent EP1572962.
JD470778 - Sequence 451802 from Patent EP1572962.
JD407344 - Sequence 388368 from Patent EP1572962.
JD094659 - Sequence 75683 from Patent EP1572962.
JD370372 - Sequence 351396 from Patent EP1572962.
MA606652 - JP 2018138019-A/178578: Polycomb-Associated Non-Coding RNAs.
MA606653 - JP 2018138019-A/178579: Polycomb-Associated Non-Coding RNAs.
MA606654 - JP 2018138019-A/178580: Polycomb-Associated Non-Coding RNAs.
MA606655 - JP 2018138019-A/178581: Polycomb-Associated Non-Coding RNAs.
MA606656 - JP 2018138019-A/178582: Polycomb-Associated Non-Coding RNAs.
MA606657 - JP 2018138019-A/178583: Polycomb-Associated Non-Coding RNAs.
MA606658 - JP 2018138019-A/178584: Polycomb-Associated Non-Coding RNAs.
MA606659 - JP 2018138019-A/178585: Polycomb-Associated Non-Coding RNAs.
MA443602 - JP 2018138019-A/15528: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9Y4H2 (Reactome details) participates in the following event(s):

R-HSA-2428922 IGF1,2:p-Y1161,1165,1166-IGF1R binds IRS2
R-HSA-74707 Binding of IRS to insulin receptor
R-HSA-74747 Dephosphorylation of p-Y-IRS1,p-Y-IRS2
R-HSA-198211 Active TRKA binds IRS1/2
R-HSA-1168423 JAK2 phosphorylation of IRS-1/2
R-HSA-2671873 Phosphorylated LEP:LEPR:JAK2:SH2B1 Binds IRS1/2
R-HSA-8855915 2x p-5Y-RET:GDNF:GFRA complexes bind RET interactors
R-HSA-74736 GRB2-1:SOS1 binds p-IRS1,p-IRS2
R-HSA-74737 p-Y-IRS1,p-Y-IRS2 bind PI3K
R-HSA-74712 Dissociation of IRS-P from insulin receptor
R-HSA-5686315 GRB2:p-4S-SOS1:p-Y-IRS1,p-Y-IRS2 dissociates
R-HSA-74711 Phosphorylation of IRS
R-HSA-198295 TRKA phosphorylates IRS
R-HSA-2671862 JAK2 Phosphorylates IRS in Response to Leptin
R-HSA-109823 MAPK3,(MAPK1) phosphorylates GRB2-1:SOS1:p-Y-IRS1,p-Y-IRS2
R-HSA-198315 Active IRS recruits PI3K to the plasma membrane and activates it
R-HSA-2428926 IGF1,2:p-Y1161,1165,1166-IGF1R phosphorylates IRS1,2,4
R-HSA-109817 GRB2:SOS1:p-Y-IRS1,p-Y-IRS2 mediated nucleotide exchange of RAS
R-HSA-198266 PI3K produces PIP3 and other phosphatidyl inositides
R-HSA-109699 PI3K-containing complexes phosphorylate PIP2 to PIP3
R-HSA-5672965 RAS GEFs promote RAS nucleotide exchange
R-HSA-2400009 PI3K inhibitors block PI3K catalytic activity
R-HSA-2316434 PI3K phosphorylates PIP2 to PIP3
R-HSA-2428928 IRS-related events triggered by IGF1R
R-HSA-74713 IRS activation
R-HSA-1266695 Interleukin-7 signaling
R-HSA-74749 Signal attenuation
R-HSA-198203 PI3K/AKT activation
R-HSA-982772 Growth hormone receptor signaling
R-HSA-2586552 Signaling by Leptin
R-HSA-8853659 RET signaling
R-HSA-2428924 IGF1R signaling cascade
R-HSA-112412 SOS-mediated signalling
R-HSA-109704 PI3K Cascade
R-HSA-74751 Insulin receptor signalling cascade
R-HSA-449147 Signaling by Interleukins
R-HSA-187037 Signaling by NTRK1 (TRKA)
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-162582 Signal Transduction
R-HSA-422475 Axon guidance
R-HSA-2404192 Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
R-HSA-112399 IRS-mediated signalling
R-HSA-74752 Signaling by Insulin receptor
R-HSA-166520 Signaling by NTRKs
R-HSA-168256 Immune System
R-HSA-1266738 Developmental Biology
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-2219530 Constitutive Signaling by Aberrant PI3K in Cancer
R-HSA-1257604 PIP3 activates AKT signaling
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-2219528 PI3K/AKT Signaling in Cancer
R-HSA-9006925 Intracellular signaling by second messengers
R-HSA-199418 Negative regulation of the PI3K/AKT network
R-HSA-5683057 MAPK family signaling cascades
R-HSA-5663202 Diseases of signal transduction
R-HSA-1643685 Disease

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000375856.1, ENST00000375856.2, ENST00000375856.3, ENST00000375856.4, IRS2_HUMAN, NM_003749, Q96RR2, Q9BZG0, Q9Y4H2, Q9Y6I5, uc318moh.1, uc318moh.2
UCSC ID: ENST00000375856.5_7
RefSeq Accession: NM_003749.3
Protein: Q9Y4H2 (aka IRS2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.