Human Gene ISL2 (ENST00000290759.9_4) from GENCODE V47lift37
  Description: ISL LIM homeobox 2 (from RefSeq NM_145805.3)
Gencode Transcript: ENST00000290759.9_4
Gencode Gene: ENSG00000159556.10_7
Transcript (Including UTRs)
   Position: hg19 chr15:76,629,114-76,634,816 Size: 5,703 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr15:76,629,225-76,634,176 Size: 4,952 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:76,629,114-76,634,816)mRNA (may differ from genome)Protein (359 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ISL2_HUMAN
DESCRIPTION: RecName: Full=Insulin gene enhancer protein ISL-2; Short=Islet-2;
FUNCTION: Transcriptional factor that defines subclasses of motoneurons that segregate into columns in the spinal cord and select distinct axon pathways (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Contains 1 homeobox DNA-binding domain.
SIMILARITY: Contains 2 LIM zinc-binding domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.05 RPKM in Pituitary
Total median expression: 23.40 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -35.70111-0.322 Picture PostScript Text
3' UTR -196.60640-0.307 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR017970 - Homeobox_CS
IPR001356 - Homeodomain
IPR009057 - Homeodomain-like
IPR001781 - Znf_LIM

Pfam Domains:
PF00046 - Homeodomain
PF00412 - LIM domain
PF05920 - Homeobox KN domain

SCOP Domains:
46689 - Homeodomain-like
57716 - Glucocorticoid receptor-like (DNA-binding domain)

ModBase Predicted Comparative 3D Structure on Q96A47
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003677 DNA binding
GO:0043565 sequence-specific DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0007275 multicellular organism development
GO:0021520 spinal cord motor neuron cell fate specification
GO:0021524 visceral motor neuron differentiation
GO:0031290 retinal ganglion cell axon guidance
GO:0045665 negative regulation of neuron differentiation
GO:0048663 neuron fate commitment
GO:0048666 neuron development
GO:0048935 peripheral nervous system neuron development

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  LF213152 - JP 2014500723-A/20655: Polycomb-Associated Non-Coding RNAs.
LF208501 - JP 2014500723-A/16004: Polycomb-Associated Non-Coding RNAs.
LF212280 - JP 2014500723-A/19783: Polycomb-Associated Non-Coding RNAs.
AB593133 - Homo sapiens ISL2 mRNA for insulin gene enhancer protein ISL-2, complete cds, clone: HP08667-RBd80H05.
AK001022 - Homo sapiens cDNA FLJ10160 fis, clone HEMBA1003545, highly similar to INSULIN GENE ENHANCER PROTEIN ISL-2.
BC012136 - Homo sapiens ISL LIM homeobox 2, mRNA (cDNA clone MGC:20227 IMAGE:4558235), complete cds.
BC011967 - Homo sapiens ISL LIM homeobox 2, mRNA (cDNA clone MGC:9275 IMAGE:3866562), complete cds.
JD227383 - Sequence 208407 from Patent EP1572962.
EU446846 - Synthetic construct Homo sapiens clone IMAGE:100069903; IMAGE:100012055; FLH257075.01L ISL LIM homeobox 2 (ISL2) gene, encodes complete protein.
JD129563 - Sequence 110587 from Patent EP1572962.
JD239919 - Sequence 220943 from Patent EP1572962.
JD455284 - Sequence 436308 from Patent EP1572962.
JD488903 - Sequence 469927 from Patent EP1572962.
JD157488 - Sequence 138512 from Patent EP1572962.
JD405665 - Sequence 386689 from Patent EP1572962.
JD218064 - Sequence 199088 from Patent EP1572962.
JD433978 - Sequence 415002 from Patent EP1572962.
JD245718 - Sequence 226742 from Patent EP1572962.
MA448729 - JP 2018138019-A/20655: Polycomb-Associated Non-Coding RNAs.
MA444078 - JP 2018138019-A/16004: Polycomb-Associated Non-Coding RNAs.
MA447857 - JP 2018138019-A/19783: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: B3KM37, ENST00000290759.1, ENST00000290759.2, ENST00000290759.3, ENST00000290759.4, ENST00000290759.5, ENST00000290759.6, ENST00000290759.7, ENST00000290759.8, ISL2_HUMAN, NM_145805, Q96A47, uc317kwb.1, uc317kwb.2
UCSC ID: ENST00000290759.9_4
RefSeq Accession: NM_145805.3
Protein: Q96A47 (aka ISL2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.