ID:ITSN2_HUMAN DESCRIPTION: RecName: Full=Intersectin-2; AltName: Full=SH3 domain-containing protein 1B; AltName: Full=SH3P18; AltName: Full=SH3P18-like WASP-associated protein; FUNCTION: Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). SUBUNIT: Belongs to a complex that may contain multimers of ITSN1, ITSN2 and EPS15, and different partners according to the step in the endocytic process. Interacts with ADAM15. Interacts with FASLG. Interacts with ANKRD54 (By similarity). Interacts with FCHO2. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Ubiquitous. Isoform 1 is primarily expressed in adult heart and liver. MISCELLANEOUS: Overexpression results in the inhibition of the transferrin uptake and the blockage of the clathrin-mediated endocytosis. SIMILARITY: Contains 1 C2 domain. SIMILARITY: Contains 1 DH (DBL-homology) domain. SIMILARITY: Contains 2 EF-hand domains. SIMILARITY: Contains 2 EH domains. SIMILARITY: Contains 1 PH domain. SIMILARITY: Contains 5 SH3 domains. CAUTION: It is uncertain whether Met-1 or Met-2 is the initiator. CAUTION: Studies in clathrin-mediated endocytosis of ITGB1 and TFR used a siRNA mixture of ISTN1 and ISTN2 suggesting a partially overlapping role of the EH domain-containing proteins (PubMed:22648170). SEQUENCE CAUTION: Sequence=AAF59903.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAF59904.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAF63600.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAA86570.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9NZM3
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0005070 SH3/SH2 adaptor activity GO:0005089 Rho guanyl-nucleotide exchange factor activity GO:0005509 calcium ion binding GO:0005515 protein binding GO:0046872 metal ion binding
Biological Process: GO:0006897 endocytosis GO:0009967 positive regulation of signal transduction GO:0030154 cell differentiation GO:0035023 regulation of Rho protein signal transduction GO:1903861 positive regulation of dendrite extension