Human Gene KLHL9 (ENST00000359039.5_7) from GENCODE V47lift37
  Description: kelch like family member 9 (from RefSeq NM_018847.4)
Gencode Transcript: ENST00000359039.5_7
Gencode Gene: ENSG00000198642.8_10
Transcript (Including UTRs)
   Position: hg19 chr9:21,329,664-21,335,403 Size: 5,740 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr9:21,333,005-21,334,858 Size: 1,854 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr9:21,329,664-21,335,403)mRNA (may differ from genome)Protein (617 aa)
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UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KLHL9_HUMAN
DESCRIPTION: RecName: Full=Kelch-like protein 9;
FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts with AURKB.
SIMILARITY: Contains 1 BACK (BTB/Kelch associated) domain.
SIMILARITY: Contains 1 BTB (POZ) domain.
SIMILARITY: Contains 6 Kelch repeats.
SEQUENCE CAUTION: Sequence=BAA92592.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: KLHL9
Diseases sorted by gene-association score: klhl9-related early-onset distal myopathy* (350)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.74 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 311.64 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -224.60545-0.412 Picture PostScript Text
3' UTR -821.203341-0.246 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011705 - BACK
IPR000210 - BTB/POZ-like
IPR011333 - BTB/POZ_fold
IPR013069 - BTB_POZ
IPR015916 - Gal_Oxidase_b-propeller
IPR017096 - Kelch-like_gigaxonin
IPR006652 - Kelch_1

Pfam Domains:
PF00651 - BTB/POZ domain
PF01344 - Kelch motif
PF07707 - BTB And C-terminal Kelch
PF13415 - Galactose oxidase, central domain
PF13418 - Galactose oxidase, central domain
PF13964 - Kelch motif

SCOP Domains:
117281 - Kelch motif
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50985 - RCC1/BLIP-II
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
54695 - POZ domain

ModBase Predicted Comparative 3D Structure on Q9P2J3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004842 ubiquitin-protein transferase activity

Biological Process:
GO:0007049 cell cycle
GO:0016567 protein ubiquitination
GO:0032465 regulation of cytokinesis
GO:0043687 post-translational protein modification
GO:0051301 cell division

Cellular Component:
GO:0005829 cytosol
GO:0030496 midbody
GO:0031463 Cul3-RING ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  KJ899219 - Synthetic construct Homo sapiens clone ccsbBroadEn_08613 KLHL9 gene, encodes complete protein.
BC064958 - Homo sapiens cDNA clone IMAGE:6093326, partial cds.
AY172949 - Homo sapiens KLHL9 protein mRNA, complete cds.
BC052776 - Homo sapiens kelch-like 9 (Drosophila), mRNA (cDNA clone IMAGE:4821138).
JD212885 - Sequence 193909 from Patent EP1572962.
JD463018 - Sequence 444042 from Patent EP1572962.
AK025468 - Homo sapiens cDNA: FLJ21815 fis, clone HEP01095.
AK023630 - Homo sapiens cDNA FLJ13568 fis, clone PLACE1008368, weakly similar to RING CANAL PROTEIN.
AB037775 - Homo sapiens KIAA1354 mRNA for KIAA1354 protein.
AL713669 - Homo sapiens mRNA; cDNA DKFZp761G20121 (from clone DKFZp761G20121).
JD053337 - Sequence 34361 from Patent EP1572962.
JD451359 - Sequence 432383 from Patent EP1572962.
JD233641 - Sequence 214665 from Patent EP1572962.
JD188068 - Sequence 169092 from Patent EP1572962.
JD050160 - Sequence 31184 from Patent EP1572962.
BX538121 - Homo sapiens mRNA; cDNA DKFZp686L0695 (from clone DKFZp686L0695); complete cds.
JD436439 - Sequence 417463 from Patent EP1572962.
JD345302 - Sequence 326326 from Patent EP1572962.
JD350406 - Sequence 331430 from Patent EP1572962.
BC039133 - Homo sapiens kelch-like 9 (Drosophila), mRNA (cDNA clone MGC:16975 IMAGE:4339111), complete cds.
AK091715 - Homo sapiens cDNA FLJ34396 fis, clone HCHON2000826, highly similar to Kelch-like protein 9.
JD083894 - Sequence 64918 from Patent EP1572962.
JD110744 - Sequence 91768 from Patent EP1572962.
JD052177 - Sequence 33201 from Patent EP1572962.
JD239153 - Sequence 220177 from Patent EP1572962.
JD040381 - Sequence 21405 from Patent EP1572962.
JD083431 - Sequence 64455 from Patent EP1572962.
JD240659 - Sequence 221683 from Patent EP1572962.
JD429874 - Sequence 410898 from Patent EP1572962.
BC113513 - Homo sapiens kelch-like 9 (Drosophila), mRNA (cDNA clone MGC:142073 IMAGE:8322565), complete cds.
BC105008 - Homo sapiens kelch-like 9 (Drosophila), mRNA (cDNA clone MGC:132668 IMAGE:8144011), complete cds.
JD311127 - Sequence 292151 from Patent EP1572962.
JD293619 - Sequence 274643 from Patent EP1572962.
JD216951 - Sequence 197975 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9P2J3 (Reactome details) participates in the following event(s):

R-HSA-8955241 CAND1 binds cytosolic CRL E3 ubiquitin ligases
R-HSA-983157 Interaction of E3 with substrate and E2-Ub complex
R-HSA-983147 Release of E3 from polyubiquitinated substrate
R-HSA-8955289 COMMDs displace CAND1 from cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-8956040 COP9 signalosome deneddylates cytosolic CRL E3 ubiquitin ligase complexes
R-HSA-983140 Transfer of Ub from E2 to substrate and release of E2
R-HSA-8951664 Neddylation
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
R-HSA-597592 Post-translational protein modification
R-HSA-983169 Class I MHC mediated antigen processing & presentation
R-HSA-392499 Metabolism of proteins
R-HSA-1280218 Adaptive Immune System
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000359039.1, ENST00000359039.2, ENST00000359039.3, ENST00000359039.4, KIAA1354, KLHL9_HUMAN, NM_018847, Q8TCQ2, Q9P2J3, uc318avx.1, uc318avx.2
UCSC ID: ENST00000359039.5_7
RefSeq Accession: NM_018847.4
Protein: Q9P2J3 (aka KLHL9_HUMAN or KHL9_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.