Human Gene KRT28 (ENST00000306658.8_7) from GENCODE V47lift37
  Description: keratin 28 (from RefSeq NM_181535.3)
Gencode Transcript: ENST00000306658.8_7
Gencode Gene: ENSG00000173908.9_9
Transcript (Including UTRs)
   Position: hg19 chr17:38,948,448-38,956,211 Size: 7,764 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg19 chr17:38,948,679-38,956,145 Size: 7,467 Coding Exon Count: 8 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:38,948,448-38,956,211)mRNA (may differ from genome)Protein (464 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: K1C28_HUMAN
DESCRIPTION: RecName: Full=Keratin, type I cytoskeletal 28; AltName: Full=Cytokeratin-28; Short=CK-28; AltName: Full=Keratin-25D; Short=K25D; AltName: Full=Keratin-28; Short=K28; AltName: Full=Type I inner root sheath-specific keratin-K25irs4;
FUNCTION: Essential for the proper assembly of types I and II keratin protein complexes and the formation of keratin intermediate filaments in the inner root sheath (irs) (By similarity).
SUBUNIT: Heterotetramer of two type I and two type II keratins.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Strongly expressed in skin and scalp, and weak expression observed in thymus. In the hair follicle, expressed in Henle layer, Huxley layer and in the irs cuticle. Expression extends from the bulb region up to the point of differentiation into the three layers. Also present in the medulla of beard hair (at protein level).
MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
SIMILARITY: Belongs to the intermediate filament family.
SEQUENCE CAUTION: Sequence=BAC85240.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.27 RPKM in Testis
Total median expression: 0.29 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -8.1066-0.123 Picture PostScript Text
3' UTR -33.30231-0.144 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016044 - F
IPR001664 - IF
IPR002957 - Keratin_I

Pfam Domains:
PF00038 - Intermediate filament protein

SCOP Domains:
64593 - Intermediate filament protein, coiled coil region
90257 - Myosin rod fragments

ModBase Predicted Comparative 3D Structure on Q7Z3Y7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0005198 structural molecule activity

Biological Process:
GO:0008150 biological_process
GO:0031424 keratinization
GO:0070268 cornification

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005882 intermediate filament
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK129827 - Homo sapiens cDNA FLJ26317 fis, clone DMC09625.
JD501311 - Sequence 482335 from Patent EP1572962.
AJ564207 - Homo sapiens mRNA for type I inner root sheath specific keratin 25 irs4 (KRT25D gene).
BC146368 - Synthetic construct Homo sapiens clone IMAGE:100015084, MGC:180196 keratin 28 (KRT28) mRNA, encodes complete protein.
BC148794 - Synthetic construct Homo sapiens clone IMAGE:100015900, MGC:183211 keratin 28 (KRT28) mRNA, encodes complete protein.
AB463813 - Synthetic construct DNA, clone: pF1KE0560, Homo sapiens KRT28 gene for keratin 28, without stop codon, in Flexi system.
JD114229 - Sequence 95253 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q7Z3Y7 (Reactome details) participates in the following event(s):

R-HSA-6805546 Keratin type I binds keratin type II
R-HSA-6805573 Keratin type I/type II heterodimers form tetramers
R-HSA-6806613 Keratin tetramers bind to form unit length filaments
R-HSA-6805567 Keratinization
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000306658.1, ENST00000306658.2, ENST00000306658.3, ENST00000306658.4, ENST00000306658.5, ENST00000306658.6, ENST00000306658.7, K1C28_HUMAN, KRT25D , KRT28 , NM_181535, Q6ZP84, Q7Z3Y7, uc317nwz.1, uc317nwz.2
UCSC ID: ENST00000306658.8_7
RefSeq Accession: NM_181535.3
Protein: Q7Z3Y7 (aka K1C28_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.