ID:LEO1_HUMAN DESCRIPTION: RecName: Full=RNA polymerase-associated protein LEO1; AltName: Full=Replicative senescence down-regulated leo1-like protein; FUNCTION: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Involved in polyadenylation of mRNA precursors. Connects PAF1C to Wnt signaling. SUBUNIT: Component of the PAF1 complex, which consists of CDC73, PAF1, LEO1, CTR9, RTF1 and WDR61. Interacts with TCEA1, SUPT5H and CTNNB1. INTERACTION: Q6P1J9:CDC73; NbExp=11; IntAct=EBI-932432, EBI-930143; P35222:CTNNB1; NbExp=2; IntAct=EBI-932432, EBI-491549; Q8N7H5:PAF1; NbExp=17; IntAct=EBI-932432, EBI-2607770; P23193:TCEA1; NbExp=4; IntAct=EBI-932432, EBI-2608271; SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Highly expressed in skeletal muscle and heart. Weakly expressed in placenta and liver. SIMILARITY: Belongs to the LEO1 family. SEQUENCE CAUTION: Sequence=BAB71006.1; Type=Frameshift; Positions=473;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8WVC0
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC018147 - Homo sapiens Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae), mRNA (cDNA clone MGC:9690 IMAGE:3847809), complete cds. AY302186 - Homo sapiens replicative senescence downregulated leo1-like protein mRNA, complete cds. AK055762 - Homo sapiens cDNA FLJ31200 fis, clone KIDNE2000574, weakly similar to Staphylococcus epidermidis putative cell-surface adhesin SdrF gene. AK297972 - Homo sapiens cDNA FLJ60738 complete cds, highly similar to RNA polymerase-associated protein LEO1. KJ895376 - Synthetic construct Homo sapiens clone ccsbBroadEn_04770 LEO1 gene, encodes complete protein.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q8WVC0 (Reactome details) participates in the following event(s):
R-HSA-3322424 Beta-catenin recruits CDC73 and LEO1 R-HSA-112379 Recruitment of elongation factors to form elongation complex R-HSA-8942099 RNF20:RNF40 binds PAF complex, Ubiquitin:UBE2A,B (Ubiquitin:RAD6), WAC and Histone H2B R-HSA-113429 Elongating transcript encounters a lesion in the template R-HSA-112385 Addition of nucleotides leads to transcript elongation R-HSA-113411 2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing R-HSA-113412 Pol II elongation complex moves on the template as transcript elongates R-HSA-113414 7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest R-HSA-112392 Resumption of elongation after recovery from pausing R-HSA-113413 TFIIS-mediated recovery of elongation from arrest R-HSA-112395 Abortive termination of elongation after arrest R-HSA-112396 Separation of elongating transcript from template R-HSA-201722 Formation of the beta-catenin:TCF transactivating complex R-HSA-112382 Formation of RNA Pol II elongation complex R-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-201681 TCF dependent signaling in response to WNT R-HSA-75955 RNA Polymerase II Transcription Elongation R-HSA-8852135 Protein ubiquitination R-HSA-73857 RNA Polymerase II Transcription R-HSA-195721 Signaling by WNT R-HSA-597592 Post-translational protein modification R-HSA-74160 Gene expression (Transcription) R-HSA-162582 Signal Transduction R-HSA-392499 Metabolism of proteins
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
