Human Gene LGALS3 (ENST00000254301.14_4) from GENCODE V47lift37
  Description: galectin 3, transcript variant 1 (from RefSeq NM_002306.4)
Gencode Transcript: ENST00000254301.14_4
Gencode Gene: ENSG00000131981.16_7
Transcript (Including UTRs)
   Position: hg19 chr14:55,595,970-55,612,141 Size: 16,172 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr14:55,604,092-55,611,989 Size: 7,898 Coding Exon Count: 5 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:55,595,970-55,612,141)mRNA (may differ from genome)Protein (250 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: LEG3_HUMAN
DESCRIPTION: RecName: Full=Galectin-3; Short=Gal-3; AltName: Full=35 kDa lectin; AltName: Full=Carbohydrate-binding protein 35; Short=CBP 35; AltName: Full=Galactose-specific lectin 3; AltName: Full=Galactoside-binding protein; Short=GALBP; AltName: Full=IgE-binding protein; AltName: Full=L-31; AltName: Full=Laminin-binding protein; AltName: Full=Lectin L-29; AltName: Full=Mac-2 antigen;
FUNCTION: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells.
SUBUNIT: Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface.
TISSUE SPECIFICITY: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages.
SIMILARITY: Contains 1 galectin domain.
WEB RESOURCE: Name=Functional Glycomics Gateway - Glycan Binding; Note=Galectin-3; URL="http://www.functionalglycomics.org/glycomics/GBPServlet?&operationType=view&cbpId=cbp_hum_Stlect_00118";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: LGALS3
Diseases sorted by gene-association score: follicular adenoma (25), papillary carcinoma (20), thyroid cancer (18), thyroid cancer, nonmedullary, 1 (15), nodular goiter (14), histiocytic sarcoma (12), neonatal candidiasis (10), chordoma (10), cow milk allergy (10), common wart (8), hashimoto thyroiditis (8), al amyloidosis (8), parathyroid carcinoma (8), mucinous bronchioloalveolar adenocarcinoma (7), venezuelan equine encephalitis (7), thyroid cancer, nonmedullary, 2 (7), multicystic dysplastic kidney (7), cardiac sarcoidosis (6), oxyphilic adenoma (6), hypothyroidism, congenital, due to thyroid dysgenesis or hypoplasia (6), malignant struma ovarii (6), polymorphous low-grade adenocarcinoma (5), fish allergy (5), colorectal cancer (4), secretory meningioma (4), prostate cancer (2), endocrine gland cancer (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 401.92 RPKM in Colon - Transverse
Total median expression: 4662.70 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -10.1053-0.191 Picture PostScript Text
3' UTR -28.40152-0.187 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008985 - ConA-like_lec_gl_sf
IPR013320 - ConA-like_subgrp
IPR015534 - Galectin_3
IPR001079 - Galectin_CRD

Pfam Domains:
PF00337 - Galactoside-binding lectin

SCOP Domains:
49899 - Concanavalin A-like lectins/glucanases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1A3K - X-ray MuPIT 1KJL - X-ray MuPIT 1KJR - X-ray MuPIT 2NMN - X-ray MuPIT 2NMO - X-ray MuPIT 2NN8 - X-ray MuPIT 2XG3 - X-ray 3AYA - X-ray MuPIT 3AYC - X-ray MuPIT 3AYD - X-ray MuPIT 3AYE - X-ray MuPIT 3T1L - X-ray MuPIT 3T1M - X-ray MuPIT 3ZSJ - X-ray MuPIT 3ZSK - X-ray MuPIT 3ZSL - X-ray MuPIT 3ZSM - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P17931
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0019863 IgE binding
GO:0030246 carbohydrate binding
GO:0042056 chemoattractant activity
GO:0043236 laminin binding
GO:0070492 oligosaccharide binding

Biological Process:
GO:0002376 immune system process
GO:0002548 monocyte chemotaxis
GO:0006397 mRNA processing
GO:0008380 RNA splicing
GO:0030154 cell differentiation
GO:0030593 neutrophil chemotaxis
GO:0030855 epithelial cell differentiation
GO:0031640 killing of cells of other organism
GO:0042129 regulation of T cell proliferation
GO:0043312 neutrophil degranulation
GO:0045087 innate immune response
GO:0045637 regulation of myeloid cell differentiation
GO:0045806 negative regulation of endocytosis
GO:0048245 eosinophil chemotaxis
GO:0048246 macrophage chemotaxis
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050918 positive chemotaxis
GO:0070232 regulation of T cell apoptotic process
GO:0071674 mononuclear cell migration
GO:0071677 positive regulation of mononuclear cell migration
GO:0090280 positive regulation of calcium ion import
GO:1902041 regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:2000521 negative regulation of immunological synapse formation
GO:2001189 negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
GO:2001237 negative regulation of extrinsic apoptotic signaling pathway
GO:2001200 positive regulation of dendritic cell differentiation

Cellular Component:
GO:0001772 immunological synapse
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005681 spliceosomal complex
GO:0005737 cytoplasm
GO:0005743 mitochondrial inner membrane
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0030667 secretory granule membrane
GO:0031012 extracellular matrix
GO:0044216 other organism cell
GO:0070062 extracellular exosome
GO:0101003 ficolin-1-rich granule membrane
GO:1990724 galectin complex


-  Descriptions from all associated GenBank mRNAs
  HV708947 - JP 2012506450-A/45: Methods for treating eye disorders.
HW061208 - JP 2012529430-A/83: METHODS FOR TREATING CHRONIC KIDNEY DISEASE.
HW061210 - JP 2012529430-A/85: METHODS FOR TREATING CHRONIC KIDNEY DISEASE.
JA482163 - Sequence 146 from Patent WO2011072091.
JA482164 - Sequence 147 from Patent WO2011072091.
JB252016 - Sequence 83 from Patent EP2440214.
JB252018 - Sequence 85 from Patent EP2440214.
JE980455 - Sequence 146 from Patent EP2862929.
JE980456 - Sequence 147 from Patent EP2862929.
LP764915 - Sequence 83 from Patent EP3276004.
LP764917 - Sequence 85 from Patent EP3276004.
AK314929 - Homo sapiens cDNA, FLJ95838, Homo sapiens lectin, galactoside-binding, soluble, 3 (galectin 3)(LGALS3), mRNA.
AB006780 - Homo sapiens mRNA for galectin-3, complete cds.
BC053667 - Homo sapiens lectin, galactoside-binding, soluble, 3, mRNA (cDNA clone MGC:61529 IMAGE:6148401), complete cds.
BC068068 - Homo sapiens lectin, galactoside-binding, soluble, 3, mRNA (cDNA clone MGC:78581 IMAGE:6428428), complete cds.
M36682 - Human non-integrin laminin-binding protein mRNA, complete cds.
BC001120 - Homo sapiens lectin, galactoside-binding, soluble, 3, mRNA (cDNA clone MGC:2058 IMAGE:3050135), complete cds.
GQ900920 - Homo sapiens clone HEL-T-32 epididymis secretory sperm binding protein mRNA, complete cds.
M35368 - Homo sapiens galactose-specific lectin (LGALS2) mRNA, complete cds.
M57710 - Human IgE-binding protein (epsilon-BP) mRNA, complete cds.
AB209391 - Homo sapiens mRNA for LGALS3 protein variant protein.
BX641090 - Homo sapiens mRNA; cDNA DKFZp686K04237 (from clone DKFZp686K04237).
HW061209 - JP 2012529430-A/84: METHODS FOR TREATING CHRONIC KIDNEY DISEASE.
JB252017 - Sequence 84 from Patent EP2440214.
LP764916 - Sequence 84 from Patent EP3276004.
BX161455 - human full-length cDNA clone CS0DI041YE05 of Placenta of Homo sapiens (human).
HV708948 - JP 2012506450-A/46: Methods for treating eye disorders.
BC128253 - Homo sapiens cDNA clone IMAGE:40115198.
BC128403 - Homo sapiens cDNA clone IMAGE:40115197.
M64303 - Human galactoside-binding protein mRNA.
AB527899 - Synthetic construct DNA, clone: pF1KB0909, Homo sapiens LGALS3 gene for lectin, galactoside-binding, soluble, 3, without stop codon, in Flexi system.
AB528312 - Synthetic construct DNA, clone: pF1KB6301, Homo sapiens LGALS3 gene for lectin, galactoside-binding, soluble, 3, without stop codon, in Flexi system.
CR542097 - Homo sapiens full open reading frame cDNA clone RZPDo834C0937D for gene LGALS3, lectin, galactoside-binding, soluble, 3 (galectin 3); complete cds, without stopcodon.
CU691024 - Synthetic construct Homo sapiens gateway clone IMAGE:100021627 5' read LGALS3 mRNA.
EU176706 - Synthetic construct Homo sapiens clone IMAGE:100011500; FLH187917.01L; RZPDo839D06255D lectin, galactoside-binding, soluble, 3 (LGALS3) gene, encodes complete protein.
KJ905820 - Synthetic construct Homo sapiens clone ccsbBroadEn_15490 LGALS3 gene, encodes complete protein.
DQ892594 - Synthetic construct clone IMAGE:100005224; FLH187918.01X; RZPDo839E06150D lectin, galactoside-binding, soluble, 3 (galectin 3) (LGALS3) gene, encodes complete protein.
KJ897127 - Synthetic construct Homo sapiens clone ccsbBroadEn_06521 LGALS3 gene, encodes complete protein.
CR456897 - Homo sapiens full open reading frame cDNA clone RZPDo834D037D for gene LGALS3, lectin, galactoside-binding, soluble, 3 (galectin 3); complete cds, incl. stopcodon.
S59012 - carbohydrate binding protein 35=epithelial-specific lectin [human, normal colonic mucosa, colon carcinoma, cell line clone A, mRNA Partial, 753 nt].
AF266280 - Homo sapiens cytogaligin and mitochondrial mitogaligin (GALIG) mRNA, nuclear gene, complete cds.
DL491816 - Novel nucleic acids.
DL491433 - Novel nucleic acids.
DL490055 - Novel nucleic acids.
JD283509 - Sequence 264533 from Patent EP1572962.
JD359671 - Sequence 340695 from Patent EP1572962.
MB483359 - JP 2019528312-A/20: MRNA-MEDIATED IMMUNIZATION METHODS.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P17931 (Reactome details) participates in the following event(s):

R-HSA-879358 AGER-1,2,3 bind AGEs
R-HSA-6800426 Exocytosis of ficolin-rich granule membrane proteins
R-HSA-6798743 Exocytosis of secretory granule membrane proteins
R-HSA-8939246 RUNX1 regulates transcription of genes involved in differentiation of myeloid cells
R-HSA-8941333 RUNX2 regulates genes involved in differentiation of myeloid cells
R-HSA-879415 Advanced glycosylation endproduct receptor signaling
R-HSA-6798695 Neutrophil degranulation
R-HSA-8878171 Transcriptional regulation by RUNX1
R-HSA-8878166 Transcriptional regulation by RUNX2
R-HSA-168249 Innate Immune System
R-HSA-212436 Generic Transcription Pathway
R-HSA-168256 Immune System
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: B2RC38, ENST00000254301.1, ENST00000254301.10, ENST00000254301.11, ENST00000254301.12, ENST00000254301.13, ENST00000254301.2, ENST00000254301.3, ENST00000254301.4, ENST00000254301.5, ENST00000254301.6, ENST00000254301.7, ENST00000254301.8, ENST00000254301.9, LEG3_HUMAN, LGALS3 , MAC2, NM_002306, P17931, Q16005, Q6IBA7, Q96J47, uc317fnx.1, uc317fnx.2
UCSC ID: ENST00000254301.14_4
RefSeq Accession: NM_002306.4
Protein: P17931 (aka LEG3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.