ID:MD2L2_HUMAN DESCRIPTION: RecName: Full=Mitotic spindle assembly checkpoint protein MAD2B; AltName: Full=Mitotic arrest deficient 2-like protein 2; Short=MAD2-like protein 2; AltName: Full=REV7 homolog; Short=hREV7; FUNCTION: Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. SUBUNIT: Homooligomer (Probable). Interacts with REV1. Interacts with ADAM9. Interacts with CHAMP1. Interacts with REV3L. Interacts with FZR1 (in complex with the anaphase promoting complex APC). Interacts with CDC20; PubMed:11459825 could not detect the interaction. Interacts with RAN. Interacts with ELK1; the interaction is direct and recruits MAD2L2 to ELK1-specific promoters. May interact with the JNK kinases MAPK8 and/or MAPK9 to stimulate ELK1 phosphorylation and transcriptional activity upon DNA damage. Interacts with TCF7L2; prevents its binding to promoters and negatively modulates its transcriptional activity. Interacts with YY1AP1. Interacts with S.flexneri protein ipaB; prevents the interaction of MAD2L2 with FZR1 and CDC20 resulting in an activation of the anaphase-promoting complex APC and a cell cycle arrest. Interacts with PRCC; the interaction is direct. Interacts with POGZ. INTERACTION: Q13443:ADAM9; NbExp=3; IntAct=EBI-77889, EBI-77903; Q12834:CDC20; NbExp=2; IntAct=EBI-77889, EBI-367462; P30260:CDC27; NbExp=2; IntAct=EBI-77889, EBI-994813; Q9UM11:FZR1; NbExp=2; IntAct=EBI-77889, EBI-724997; P18011:ipaB (xeno); NbExp=7; IntAct=EBI-77889, EBI-490239; SUBCELLULAR LOCATION: Nucleus. Cytoplasm, cytoskeleton, spindle. Cytoplasm. TISSUE SPECIFICITY: Ubiquitously expressed. SIMILARITY: Contains 1 HORMA domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mad2l2/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9UI95
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0001102 RNA polymerase II activating transcription factor binding GO:0005515 protein binding GO:0008432 JUN kinase binding
Biological Process: GO:0000122 negative regulation of transcription from RNA polymerase II promoter GO:0001558 regulation of cell growth GO:0006281 DNA repair GO:0006302 double-strand break repair GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006974 cellular response to DNA damage stimulus GO:0007015 actin filament organization GO:0007049 cell cycle GO:0007094 mitotic spindle assembly checkpoint GO:0010719 negative regulation of epithelial to mesenchymal transition GO:0010944 negative regulation of transcription by competitive promoter binding GO:0033138 positive regulation of peptidyl-serine phosphorylation GO:0042177 negative regulation of protein catabolic process GO:0042276 error-prone translesion synthesis GO:0042772 DNA damage response, signal transduction resulting in transcription GO:0043433 negative regulation of sequence-specific DNA binding transcription factor activity GO:0045893 positive regulation of transcription, DNA-templated GO:0051301 cell division GO:0090090 negative regulation of canonical Wnt signaling pathway GO:1904667 negative regulation of ubiquitin protein ligase activity GO:2000048 negative regulation of cell-cell adhesion mediated by cadherin GO:2000678 negative regulation of transcription regulatory region DNA binding
BC015244 - Homo sapiens MAD2 mitotic arrest deficient-like 2 (yeast), mRNA (cDNA clone MGC:17940 IMAGE:3916742), complete cds. AF072933 - Homo sapiens Mad2-like protein mRNA, complete cds. AF139365 - Homo sapiens Mad2B protein (MAD2B) mRNA, complete cds. AF157482 - Homo sapiens Rev7p (REV7) mRNA, complete cds. AK027327 - Homo sapiens cDNA FLJ14421 fis, clone HEMBA1005621, highly similar to Mitotic spindle assembly checkpoint protein MAD2B. AK094316 - Homo sapiens cDNA FLJ36997 fis, clone BRACE2007087, highly similar to Mitotic spindle assembly checkpoint protein MAD2B. JD467241 - Sequence 448265 from Patent EP1572962. JD306837 - Sequence 287861 from Patent EP1572962. JD095303 - Sequence 76327 from Patent EP1572962. JD185146 - Sequence 166170 from Patent EP1572962. JD449417 - Sequence 430441 from Patent EP1572962. JD482295 - Sequence 463319 from Patent EP1572962. JD445585 - Sequence 426609 from Patent EP1572962. JD080960 - Sequence 61984 from Patent EP1572962. JD448253 - Sequence 429277 from Patent EP1572962. JD157533 - Sequence 138557 from Patent EP1572962. AF080398 - Homo sapiens MAD2-like protein mRNA, complete cds. CU677251 - Synthetic construct Homo sapiens gateway clone IMAGE:100018589 5' read MAD2L2 mRNA. DQ891038 - Synthetic construct clone IMAGE:100003668; FLH168737.01X; RZPDo839C0894D MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2) gene, encodes complete protein. DQ894217 - Synthetic construct Homo sapiens clone IMAGE:100008677; FLH168733.01L; RZPDo839C0893D MAD2 mitotic arrest deficient-like 2 (yeast) (MAD2L2) gene, encodes complete protein. DQ582520 - Homo sapiens piRNA piR-32632, complete sequence. JD408421 - Sequence 389445 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9UI95 (Reactome details) participates in the following event(s):
R-HSA-110322 Formation of Pol zeta complex R-HSA-5652151 REV1 recruits POLZ to (AP:Cyt)-DNA Template R-HSA-5655835 POLK forms a quaternary complex with REV1 and POLZ on damaged DNA template R-HSA-5656105 POLI simultaneously binds REV1 and monoUb:K164-PCNA at damaged DNA R-HSA-110311 POLZ extends translesion synthesis R-HSA-5655965 POLK and POLZ cooperate in elongation of mispaired primer termini R-HSA-5656158 POLZ elongates POLI-incorporated dNMP R-HSA-5655892 POLK incorporates dNMP opposite to damaged DNA base R-HSA-5656148 POLI incorporates dNMP opposite to damaged DNA base R-HSA-110312 Translesion synthesis by REV1 R-HSA-5655862 Translesion synthesis by POLK R-HSA-5656121 Translesion synthesis by POLI R-HSA-110313 Translesion synthesis by Y family DNA polymerases bypasses lesions on DNA template R-HSA-73893 DNA Damage Bypass R-HSA-73894 DNA Repair
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
