Human Gene MAP3K11 (ENST00000309100.8_7) from GENCODE V47lift37
  Description: mitogen-activated protein kinase kinase kinase 11 (from RefSeq NM_002419.4)
Gencode Transcript: ENST00000309100.8_7
Gencode Gene: ENSG00000173327.8_13
Transcript (Including UTRs)
   Position: hg19 chr11:65,365,228-65,381,692 Size: 16,465 Total Exon Count: 10 Strand: -
Coding Region
   Position: hg19 chr11:65,365,762-65,381,227 Size: 15,466 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:65,365,228-65,381,692)mRNA (may differ from genome)Protein (847 aa)
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-  Comments and Description Text from UniProtKB
  ID: M3K11_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase kinase kinase 11; EC=2.7.11.25; AltName: Full=Mixed lineage kinase 3; AltName: Full=Src-homology 3 domain-containing proline-rich kinase;
FUNCTION: Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation.
SUBUNIT: Homodimer; undergoes dimerization during activation. Interacts with MAP2K4/MKK4. Interacts with MAP2K7/MKK7 (By similarity).
INTERACTION: P60953:CDC42; NbExp=2; IntAct=EBI-49961, EBI-81752; P35240:NF2; NbExp=4; IntAct=EBI-49961, EBI-1014472;
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, centrosome. Note=Location is cell cycle dependent.
TISSUE SPECIFICITY: Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
PTM: Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
SIMILARITY: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 SH3 domain.
SEQUENCE CAUTION: Sequence=BAD92892.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 49.71 RPKM in Nerve - Tibial
Total median expression: 953.93 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -239.50465-0.515 Picture PostScript Text
3' UTR -220.60534-0.413 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR016231 - MAPKKK9/10/11
IPR015785 - MAPKKK9/10/11-like
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR008271 - Ser/Thr_kinase_AS
IPR011511 - SH3_2
IPR001452 - SH3_domain
IPR020635 - Tyr_kinase_cat_dom

Pfam Domains:
PF00018 - SH3 domain
PF00069 - Protein kinase domain
PF07653 - Variant SH3 domain
PF07714 - Protein tyrosine and serine/threonine kinase
PF14604 - Variant SH3 domain

SCOP Domains:
50044 - SH3-domain
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q16584
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004706 JUN kinase kinase kinase activity
GO:0004709 MAP kinase kinase kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0031434 mitogen-activated protein kinase kinase binding
GO:0031435 mitogen-activated protein kinase kinase kinase binding
GO:0042802 identical protein binding
GO:0042803 protein homodimerization activity
GO:0048365 Rac GTPase binding

Biological Process:
GO:0000187 activation of MAPK activity
GO:0006468 protein phosphorylation
GO:0007017 microtubule-based process
GO:0007254 JNK cascade
GO:0007256 activation of JNKK activity
GO:0007257 activation of JUN kinase activity
GO:0008219 cell death
GO:0008283 cell proliferation
GO:0016310 phosphorylation
GO:0043065 positive regulation of apoptotic process
GO:0043507 positive regulation of JUN kinase activity
GO:0043525 positive regulation of neuron apoptotic process
GO:0046330 positive regulation of JNK cascade
GO:0046777 protein autophosphorylation

Cellular Component:
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  U07747 - Human SH3 domain-containing proline-rich kinase (sprk) mRNA, complete cds.
AK057256 - Homo sapiens cDNA FLJ32694 fis, clone TESTI2000349, highly similar to Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25).
AK316032 - Homo sapiens cDNA, FLJ78931 complete cds, highly similar to Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25).
BC064543 - Homo sapiens mitogen-activated protein kinase kinase kinase 11, mRNA (cDNA clone MGC:74591 IMAGE:5733866), complete cds.
BC011263 - Homo sapiens mitogen-activated protein kinase kinase kinase 11, mRNA (cDNA clone MGC:17114 IMAGE:4215281), complete cds.
AK222781 - Homo sapiens mRNA for mitogen-activated protein kinase kinase kinase 11 variant, clone: HEP01062.
AK090614 - Homo sapiens cDNA FLJ33295 fis, clone BNGH42000815, highly similar to Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25).
AX746534 - Sequence 59 from Patent EP1308459.
L32976 - Human protein kinase (MLK-3) mRNA, complete cds.
JD272508 - Sequence 253532 from Patent EP1572962.
JD400150 - Sequence 381174 from Patent EP1572962.
JD076890 - Sequence 57914 from Patent EP1572962.
JD526670 - Sequence 507694 from Patent EP1572962.
JD355092 - Sequence 336116 from Patent EP1572962.
AK299609 - Homo sapiens cDNA FLJ53177 complete cds, highly similar to Mitogen-activated protein kinase kinase kinase 11 (EC 2.7.11.25).
JD459903 - Sequence 440927 from Patent EP1572962.
JD178501 - Sequence 159525 from Patent EP1572962.
JD400202 - Sequence 381226 from Patent EP1572962.
JD280333 - Sequence 261357 from Patent EP1572962.
JD120680 - Sequence 101704 from Patent EP1572962.
JD097893 - Sequence 78917 from Patent EP1572962.
JD133013 - Sequence 114037 from Patent EP1572962.
JD492545 - Sequence 473569 from Patent EP1572962.
JD156285 - Sequence 137309 from Patent EP1572962.
JD339956 - Sequence 320980 from Patent EP1572962.
JD464123 - Sequence 445147 from Patent EP1572962.
AK315557 - Homo sapiens cDNA, FLJ96628, highly similar to Homo sapiens mitogen-activated protein kinase kinase kinase 11(MAP3K11), mRNA.
AK092015 - Homo sapiens cDNA FLJ34696 fis, clone MESAN2002113, highly similar to Human SH3 domain-containing proline-rich kinase (sprk) mRNA.
AX747311 - Sequence 836 from Patent EP1308459.
AB209655 - Homo sapiens mRNA for mitogen-activated protein kinase kinase kinase 11 variant protein.
AK310986 - Homo sapiens cDNA, FLJ18028.
JD341421 - Sequence 322445 from Patent EP1572962.
JD057420 - Sequence 38444 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_mapkPathway - MAPKinase Signaling Pathway

-  Other Names for This Gene
  Alternate Gene Symbols: B4DS76, ENST00000309100.1, ENST00000309100.2, ENST00000309100.3, ENST00000309100.4, ENST00000309100.5, ENST00000309100.6, ENST00000309100.7, M3K11_HUMAN, MAP3K11 , MLK3 , NM_002419, PTK1, Q16584, Q53H00, Q59F06, Q6P2G4, SPRK , uc317oln.1, uc317oln.2
UCSC ID: ENST00000309100.8_7
RefSeq Accession: NM_002419.4
Protein: Q16584 (aka M3K11_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.