Human Gene MAPK7 (ENST00000395604.8_4) from GENCODE V47lift37
  Description: mitogen-activated protein kinase 7, transcript variant 4 (from RefSeq NM_139034.3)
Gencode Transcript: ENST00000395604.8_4
Gencode Gene: ENSG00000166484.20_9
Transcript (Including UTRs)
   Position: hg19 chr17:19,281,811-19,286,857 Size: 5,047 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr17:19,282,214-19,286,544 Size: 4,331 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:19,281,811-19,286,857)mRNA (may differ from genome)Protein (816 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MK07_HUMAN
DESCRIPTION: RecName: Full=Mitogen-activated protein kinase 7; Short=MAP kinase 7; Short=MAPK 7; EC=2.7.11.24; AltName: Full=Big MAP kinase 1; Short=BMK-1; AltName: Full=Extracellular signal-regulated kinase 5; Short=ERK-5;
FUNCTION: Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium (By similarity).
ENZYME REGULATION: Activated by tyrosine and threonine phosphorylation (By similarity). Activated in response to hyperosmolarity, hydrogen peroxide, and epidermal growth factor (EGF).
SUBUNIT: Interacts with MAP2K5. Forms oligomers (By similarity). Interacts with MEF2A, MEF2C and MEF2D; the interaction phosphorylates the MEF2s and enhances transcriptional activity of MEF2A, MEF2C but not MEF2D (By similarity). Interacts with SGK1.
INTERACTION: Q13163:MAP2K5; NbExp=2; IntAct=EBI-1213983, EBI-307294; P29590:PML; NbExp=6; IntAct=EBI-1213983, EBI-295890;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Translocates to the nucleus upon activation.
TISSUE SPECIFICITY: Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
DOMAIN: The second proline-rich region may interact with actin targeting the kinase to a specific location in the cell.
DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
PTM: Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme (By similarity). Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SEQUENCE CAUTION: Sequence=AAA81381.1; Type=Frameshift; Positions=19, 32; Sequence=BAD92848.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MAPK7
Diseases sorted by gene-association score: caroli disease (9), noma (7)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 15.83 RPKM in Cervix - Ectocervix
Total median expression: 437.38 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -53.70138-0.389 Picture PostScript Text
3' UTR -78.50313-0.251 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR003527 - MAP_kinase_CS
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
4B99 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q13164
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene Details    Gene Details
Gene Sorter    Gene Sorter
  Ensembl  SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004707 MAP kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0051019 mitogen-activated protein kinase binding

Biological Process:
GO:0000165 MAPK cascade
GO:0006468 protein phosphorylation
GO:0007049 cell cycle
GO:0007165 signal transduction
GO:0007411 axon guidance
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0019933 cAMP-mediated signaling
GO:0030154 cell differentiation
GO:0030821 negative regulation of cAMP catabolic process
GO:0034115 negative regulation of heterotypic cell-cell adhesion
GO:0036003 positive regulation of transcription from RNA polymerase II promoter in response to stress
GO:0043066 negative regulation of apoptotic process
GO:0043407 negative regulation of MAP kinase activity
GO:0045765 regulation of angiogenesis
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050728 negative regulation of inflammatory response
GO:0051247 positive regulation of protein metabolic process
GO:0051344 negative regulation of cyclic-nucleotide phosphodiesterase activity
GO:0060761 negative regulation of response to cytokine stimulus
GO:0070301 cellular response to hydrogen peroxide
GO:0070377 negative regulation of ERK5 cascade
GO:0070885 negative regulation of calcineurin-NFAT signaling cascade
GO:0071363 cellular response to growth factor stimulus
GO:0071499 cellular response to laminar fluid shear stress
GO:0071560 cellular response to transforming growth factor beta stimulus
GO:1902176 negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
GO:2000352 negative regulation of endothelial cell apoptotic process
GO:2001240 negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016605 PML body


-  Descriptions from all associated GenBank mRNAs
  BC007404 - Homo sapiens mitogen-activated protein kinase 7, mRNA (cDNA clone MGC:2148 IMAGE:3009873), complete cds.
BC007992 - Homo sapiens mitogen-activated protein kinase 7, mRNA (cDNA clone MGC:3875 IMAGE:3009873), complete cds.
U29725 - Human BMK1 alpha kinase mRNA, complete cds.
AK295376 - Homo sapiens cDNA FLJ60404 partial cds, highly similar to Mitogen-activated protein kinase 7 (EC 2.7.11.24).
U29726 - Human BMK1 beta kinase mRNA, complete cds.
BC030134 - Homo sapiens mitogen-activated protein kinase 7, mRNA (cDNA clone MGC:19581 IMAGE:4111084), complete cds.
AB209611 - Homo sapiens mRNA for Mitogen activated protein kinase 7 transcript variant 5 variant protein.
BC009963 - Homo sapiens mitogen-activated protein kinase 7, mRNA (cDNA clone MGC:15371 IMAGE:4300124), complete cds.
JD195679 - Sequence 176703 from Patent EP1572962.
JD141522 - Sequence 122546 from Patent EP1572962.
U25278 - Human ERK5 mRNA, complete cds.
AY534741 - Homo sapiens mitogen activated protein kinase 7 transcript variant 5 mRNA, complete cds.
JD365109 - Sequence 346133 from Patent EP1572962.
AB527827 - Synthetic construct DNA, clone: pF1KB5763, Homo sapiens MAPK7 gene for mitogen-activated protein kinase 7, without stop codon, in Flexi system.
HQ257997 - Synthetic construct Homo sapiens clone IMAGE:100072306 mitogen-activated protein kinase 7 (MAPK7) gene, encodes complete protein.
JD216595 - Sequence 197619 from Patent EP1572962.
JD190963 - Sequence 171987 from Patent EP1572962.
JD373470 - Sequence 354494 from Patent EP1572962.
JD334018 - Sequence 315042 from Patent EP1572962.
JD045260 - Sequence 26284 from Patent EP1572962.
JD157718 - Sequence 138742 from Patent EP1572962.
DM110803 - Analysis System for Expression of Endogenous Antisense RNA.
DM110848 - Novel Cancer Marker and Use Thereof.
JD309672 - Sequence 290696 from Patent EP1572962.
JD371082 - Sequence 352106 from Patent EP1572962.
JD334437 - Sequence 315461 from Patent EP1572962.
JD189541 - Sequence 170565 from Patent EP1572962.
JD245649 - Sequence 226673 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_erk5Pathway - Role of Erk5 in Neuronal Survival
h_hdacPathway - Control of skeletal myogenesis by HDAC & calcium/calmodulin-dependent kinase (CaMK)
h_mapkPathway - MAPKinase Signaling Pathway

Reactome (by CSHL, EBI, and GO)

Protein Q13164 (Reactome details) participates in the following event(s):

R-HSA-198714 ERK5 translocates to the nucleus
R-HSA-198733 ERK5 is activated
R-HSA-199959 ERKs are inactivated by protein phosphatase 2A
R-HSA-203797 ERKs are inactivated by dual-specific phosphatases (DUSPs)
R-HSA-199929 ERK5 activates the transcription factor MEF2
R-HSA-8855915 2x p-5Y-RET:GDNF:GFRA complexes bind RET interactors
R-HSA-198746 ERK1/2/5 activate RSK1/2/3
R-HSA-198765 Signalling to ERK5
R-HSA-187037 Signaling by NTRK1 (TRKA)
R-HSA-198753 ERK/MAPK targets
R-HSA-202670 ERKs are inactivated
R-HSA-8853659 RET signaling
R-HSA-166520 Signaling by NTRKs
R-HSA-198725 Nuclear Events (kinase and transcription factor activation)
R-HSA-450282 MAPK targets/ Nuclear events mediated by MAP kinases
R-HSA-881907 Gastrin-CREB signalling pathway via PKC and MAPK
R-HSA-2559582 Senescence-Associated Secretory Phenotype (SASP)
R-HSA-422475 Axon guidance
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-450294 MAP kinase activation
R-HSA-416476 G alpha (q) signalling events
R-HSA-2559583 Cellular Senescence
R-HSA-1266738 Developmental Biology
R-HSA-162582 Signal Transduction
R-HSA-166058 MyD88:Mal cascade initiated on plasma membrane
R-HSA-168164 Toll Like Receptor 3 (TLR3) Cascade
R-HSA-448424 Interleukin-17 signaling
R-HSA-937061 TRIF(TICAM1)-mediated TLR4 signaling
R-HSA-975138 TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation
R-HSA-975871 MyD88 cascade initiated on plasma membrane
R-HSA-388396 GPCR downstream signalling
R-HSA-2262752 Cellular responses to stress
R-HSA-166016 Toll Like Receptor 4 (TLR4) Cascade
R-HSA-168179 Toll Like Receptor TLR1:TLR2 Cascade
R-HSA-168188 Toll Like Receptor TLR6:TLR2 Cascade
R-HSA-168898 Toll-Like Receptors Cascades
R-HSA-449147 Signaling by Interleukins
R-HSA-166166 MyD88-independent TLR4 cascade
R-HSA-975155 MyD88 dependent cascade initiated on endosome
R-HSA-168142 Toll Like Receptor 10 (TLR10) Cascade
R-HSA-168176 Toll Like Receptor 5 (TLR5) Cascade
R-HSA-372790 Signaling by GPCR
R-HSA-8953897 Cellular responses to external stimuli
R-HSA-181438 Toll Like Receptor 2 (TLR2) Cascade
R-HSA-168249 Innate Immune System
R-HSA-1280215 Cytokine Signaling in Immune system
R-HSA-168138 Toll Like Receptor 9 (TLR9) Cascade
R-HSA-168181 Toll Like Receptor 7/8 (TLR7/8) Cascade
R-HSA-168256 Immune System

-  Other Names for This Gene
  Alternate Gene Symbols: BMK1, ENST00000395604.1, ENST00000395604.2, ENST00000395604.3, ENST00000395604.4, ENST00000395604.5, ENST00000395604.6, ENST00000395604.7, ERK5, MK07_HUMAN, NM_139034, PRKM7, Q13164, Q16634, Q59F50, Q6QLU7, Q7L4P4, Q969G1, Q96G51, uc318xnu.1, uc318xnu.2
UCSC ID: ENST00000395604.8_4
RefSeq Accession: NM_002749.4
Protein: Q13164 (aka MK07_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.