Human Gene MAPRE2 (ENST00000300249.10_8) from GENCODE V47lift37
  Description: microtubule associated protein RP/EB family member 2, transcript variant 5 (from RefSeq NR_046177.2)
Gencode Transcript: ENST00000300249.10_8
Gencode Gene: ENSG00000166974.13_14
Transcript (Including UTRs)
   Position: hg19 chr18:32,621,377-32,723,434 Size: 102,058 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr18:32,621,504-32,720,333 Size: 98,830 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr18:32,621,377-32,723,434)mRNA (may differ from genome)Protein (327 aa)
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-  Comments and Description Text from UniProtKB
  ID: MARE2_HUMAN
DESCRIPTION: RecName: Full=Microtubule-associated protein RP/EB family member 2; AltName: Full=APC-binding protein EB2; AltName: Full=End-binding protein 2; Short=EB2;
FUNCTION: May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration (By similarity).
SUBUNIT: Interacts with DCTN1. Binds to the C-terminal domain of APC. Binds monomeric and polymerized tubulin.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Note=Associated with the microtubule network. Accumulates at the plus end of microtubules.
TISSUE SPECIFICITY: Expressed in different tumor cell lines. Up- regulated in activated B- and T-lymphocytes.
DOMAIN: Composed of two functionally independent domains. The N- terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
SIMILARITY: Belongs to the MAPRE family.
SIMILARITY: Contains 1 CH (calponin-homology) domain.
SIMILARITY: Contains 1 EB1 C-terminal domain.
SEQUENCE CAUTION: Sequence=BAA83375.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MAPRE2
Diseases sorted by gene-association score: symmetric circumferential skin creases, congenital, 2* (1200), multiple benign circumferential skin creases on limbs* (247), adenomatous polyposis coli (17), usher syndrome, type 2a (8)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 49.52 RPKM in Brain - Spinal cord (cervical c-1)
Total median expression: 870.45 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -49.60127-0.391 Picture PostScript Text
3' UTR -793.803101-0.256 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001715 - CH-domain
IPR004953 - EB1_C

Pfam Domains:
PF00307 - Calponin homology (CH) domain
PF03271 - EB1-like C-terminal motif
PF12998 - Inhibitor of growth proteins N-terminal histone-binding

SCOP Domains:
140612 - EB1 dimerisation domain-like
47576 - Calponin-homology domain, CH-domain

ModBase Predicted Comparative 3D Structure on Q15555
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008017 microtubule binding
GO:0042802 identical protein binding

Biological Process:
GO:0007049 cell cycle
GO:0007165 signal transduction
GO:0008283 cell proliferation
GO:0051301 cell division

Cellular Component:
GO:0005737 cytoplasm
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0015630 microtubule cytoskeleton
GO:0035371 microtubule plus-end


-  Descriptions from all associated GenBank mRNAs
  AK294833 - Homo sapiens cDNA FLJ58487 complete cds, highly similar to Microtubule-associated protein RP/EB family member 2.
AK090945 - Homo sapiens cDNA FLJ33626 fis, clone BRAMY2022160, highly similar to Microtubule-associated protein RP/EB family member 2.
AX746696 - Sequence 221 from Patent EP1308459.
AK315766 - Homo sapiens cDNA, FLJ96879, Homo sapiens microtubule-associated protein, RP/EB family, member 2(MAPRE2), mRNA.
BC007318 - Homo sapiens microtubule-associated protein, RP/EB family, member 2, mRNA (cDNA clone MGC:1279 IMAGE:2989643), complete cds.
X94232 - H.sapiens mRNA for novel T-cell activation protein.
AB016823 - Homo sapiens mRNA for APC-binding protein EB1, complete cds.
CU675609 - Synthetic construct Homo sapiens gateway clone IMAGE:100018957 5' read MAPRE2 mRNA.
AB528177 - Synthetic construct DNA, clone: pF1KE0124, Homo sapiens MAPRE2 gene for microtubule-associated protein, RP/EB family, member 2, without stop codon, in Flexi system.
AM393705 - Synthetic construct Homo sapiens clone IMAGE:100002028 for hypothetical protein (MAPRE2 gene).
BT020086 - Homo sapiens microtubule-associated protein, RP/EB family, member 2 mRNA, complete cds.
DQ892923 - Synthetic construct clone IMAGE:100005553; FLH190880.01X; RZPDo839E1276D microtubule-associated protein, RP/EB family, member 2 (MAPRE2) gene, encodes complete protein.
DQ896172 - Synthetic construct Homo sapiens clone IMAGE:100010632; FLH190876.01L; RZPDo839E1266D microtubule-associated protein, RP/EB family, member 2 (MAPRE2) gene, encodes complete protein.
KJ893193 - Synthetic construct Homo sapiens clone ccsbBroadEn_02587 MAPRE2 gene, encodes complete protein.
CR536545 - Homo sapiens full open reading frame cDNA clone RZPDo834G0720D for gene MAPRE2, microtubule-associated protein, RP/EB family, member 2; complete cds, incl. stopcodon.
AK296251 - Homo sapiens cDNA FLJ54006 complete cds, highly similar to Microtubule-associated protein RP/EB family member 2.
JD453109 - Sequence 434133 from Patent EP1572962.
JD187006 - Sequence 168030 from Patent EP1572962.
JD456589 - Sequence 437613 from Patent EP1572962.
JD348756 - Sequence 329780 from Patent EP1572962.
JD513302 - Sequence 494326 from Patent EP1572962.
JD479996 - Sequence 461020 from Patent EP1572962.
JD554010 - Sequence 535034 from Patent EP1572962.
JD262718 - Sequence 243742 from Patent EP1572962.
JD094657 - Sequence 75681 from Patent EP1572962.
JD359715 - Sequence 340739 from Patent EP1572962.
JD238743 - Sequence 219767 from Patent EP1572962.
JD082809 - Sequence 63833 from Patent EP1572962.
JD515365 - Sequence 496389 from Patent EP1572962.
JD293593 - Sequence 274617 from Patent EP1572962.
JD120230 - Sequence 101254 from Patent EP1572962.
JD488967 - Sequence 469991 from Patent EP1572962.
JD410317 - Sequence 391341 from Patent EP1572962.
JD260636 - Sequence 241660 from Patent EP1572962.
JD320366 - Sequence 301390 from Patent EP1572962.
JD504055 - Sequence 485079 from Patent EP1572962.
JD232195 - Sequence 213219 from Patent EP1572962.
JD456285 - Sequence 437309 from Patent EP1572962.
JD352631 - Sequence 333655 from Patent EP1572962.
JD463637 - Sequence 444661 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B2RE21, B3KR39, B4DJV4, B7Z2L3, E9PHR3, ENST00000300249.1, ENST00000300249.2, ENST00000300249.3, ENST00000300249.4, ENST00000300249.5, ENST00000300249.6, ENST00000300249.7, ENST00000300249.8, ENST00000300249.9, F5H1V8, G5E9I6, MARE2_HUMAN, NR_046177, Q15555, Q9UQ33, RP1, uc317mos.1, uc317mos.2
UCSC ID: ENST00000300249.10_8
RefSeq Accession: NM_014268.4
Protein: Q15555 (aka MARE2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.