Human Gene MARCKS (ENST00000612661.2_6) from GENCODE V47lift37
  Description: myristoylated alanine rich protein kinase C substrate (from RefSeq NM_002356.7)
Gencode Transcript: ENST00000612661.2_6
Gencode Gene: ENSG00000277443.3_9
Transcript (Including UTRs)
   Position: hg19 chr6:114,178,524-114,184,652 Size: 6,129 Total Exon Count: 4 Strand: +
Coding Region
   Position: hg19 chr6:114,178,922-114,181,755 Size: 2,834 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:114,178,524-114,184,652)mRNA (may differ from genome)Protein (332 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MARCS_HUMAN
DESCRIPTION: RecName: Full=Myristoylated alanine-rich C-kinase substrate; Short=MARCKS; AltName: Full=Protein kinase C substrate, 80 kDa protein, light chain; Short=80K-L protein; Short=PKCSL;
FUNCTION: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (Probable). Membrane; Lipid-anchor (By similarity).
PTM: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.
SIMILARITY: Belongs to the MARCKS family.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/marcks/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MARCKS
Diseases sorted by gene-association score: anencephaly (3), spinocerebellar ataxia 14 (1), mental retardation and microcephaly with pontine and cerebellar hypoplasia (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D013749 Tetrachlorodibenzodioxin
  • D003993 Dibutyl Phthalate
  • D004958 Estradiol
  • D011374 Progesterone
  • C049325 1,2-dithiol-3-thione
  • D015056 1-Methyl-3-isobutylxanthine
  • C029790 2,2',3',4,4',5-hexachlorobiphenyl
  • C548651 2-(1'H-indolo-3'-carbonyl)thiazole-4-carboxylic acid methyl ester
  • C465664 2-(1-(3-dimethylaminopropyl)-5-methoxyindol-3-yl)-3-(1H-indol-3-yl)maleimide
  • C023035 3,4,5,3',4'-pentachlorobiphenyl
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -81.50398-0.205 Picture PostScript Text
3' UTR -641.402895-0.222 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002101 - MARCKS

Pfam Domains:
PF02063 - MARCKS family

ModBase Predicted Comparative 3D Structure on P29966
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003779 actin binding
GO:0005080 protein kinase C binding
GO:0005516 calmodulin binding
GO:0042802 identical protein binding
GO:0051015 actin filament binding

Biological Process:
GO:0051017 actin filament bundle assembly
GO:0051260 protein homooligomerization
GO:0051764 actin crosslink formation

Cellular Component:
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005856 cytoskeleton
GO:0005886 plasma membrane
GO:0005925 focal adhesion
GO:0005938 cell cortex
GO:0015629 actin cytoskeleton
GO:0016020 membrane
GO:0032432 actin filament bundle
GO:0042585 germinal vesicle
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LF208733 - JP 2014500723-A/16236: Polycomb-Associated Non-Coding RNAs.
LF322165 - JP 2014500723-A/129668: Polycomb-Associated Non-Coding RNAs.
AK074526 - Homo sapiens cDNA FLJ90045 fis, clone HEMBA1002227, highly similar to Homo sapiens mRNA for 80K-L protein.
BC028284 - Homo sapiens myristoylated alanine-rich protein kinase C substrate, mRNA (cDNA clone IMAGE:5219286), partial cds.
BC013004 - Homo sapiens myristoylated alanine-rich protein kinase C substrate, mRNA (cDNA clone IMAGE:3532926), partial cds.
BC068059 - Homo sapiens myristoylated alanine-rich protein kinase C substrate, mRNA (cDNA clone IMAGE:6265686), partial cds.
JD095052 - Sequence 76076 from Patent EP1572962.
D10522 - Homo sapiens mRNA for 80K-L protein, complete cds.
LF322163 - JP 2014500723-A/129666: Polycomb-Associated Non-Coding RNAs.
M68956 - Human myristoylated alanine-rich C-kinase substrate mRNA, complete cds.
JD301153 - Sequence 282177 from Patent EP1572962.
JD151641 - Sequence 132665 from Patent EP1572962.
BC089040 - Homo sapiens myristoylated alanine-rich protein kinase C substrate, mRNA (cDNA clone MGC:99613 IMAGE:6378349), complete cds.
JD116078 - Sequence 97102 from Patent EP1572962.
JD519406 - Sequence 500430 from Patent EP1572962.
JD200371 - Sequence 181395 from Patent EP1572962.
JD424061 - Sequence 405085 from Patent EP1572962.
JD528115 - Sequence 509139 from Patent EP1572962.
JD310006 - Sequence 291030 from Patent EP1572962.
EU832331 - Synthetic construct Homo sapiens clone HAIB:100067360; DKFZo008F0527 myristoylated alanine-rich protein kinase C substrate protein (MARCKS) gene, encodes complete protein.
EU832416 - Synthetic construct Homo sapiens clone HAIB:100067445; DKFZo004F0528 myristoylated alanine-rich protein kinase C substrate protein (MARCKS) gene, encodes complete protein.
AB463910 - Synthetic construct DNA, clone: pF1KB9451, Homo sapiens MARCKS gene for myristoylated alanine-rich protein kinase C substrate, without stop codon, in Flexi system.
JD300854 - Sequence 281878 from Patent EP1572962.
LF322162 - JP 2014500723-A/129665: Polycomb-Associated Non-Coding RNAs.
JD127005 - Sequence 108029 from Patent EP1572962.
JD530610 - Sequence 511634 from Patent EP1572962.
JD530611 - Sequence 511635 from Patent EP1572962.
LF322161 - JP 2014500723-A/129664: Polycomb-Associated Non-Coding RNAs.
JD091726 - Sequence 72750 from Patent EP1572962.
JD051458 - Sequence 32482 from Patent EP1572962.
LF322159 - JP 2014500723-A/129662: Polycomb-Associated Non-Coding RNAs.
JD315719 - Sequence 296743 from Patent EP1572962.
JD539531 - Sequence 520555 from Patent EP1572962.
LF322158 - JP 2014500723-A/129661: Polycomb-Associated Non-Coding RNAs.
JD174924 - Sequence 155948 from Patent EP1572962.
JD429388 - Sequence 410412 from Patent EP1572962.
JD237463 - Sequence 218487 from Patent EP1572962.
AK027274 - Homo sapiens cDNA FLJ14368 fis, clone HEMBA1001122.
AB020968 - Homo sapiens mRNA, 3'UTR, up-regulated by BCG-CWS.
LF322157 - JP 2014500723-A/129660: Polycomb-Associated Non-Coding RNAs.
BC039492 - Homo sapiens, clone IMAGE:5547644, mRNA.
LF322156 - JP 2014500723-A/129659: Polycomb-Associated Non-Coding RNAs.
LF322155 - JP 2014500723-A/129658: Polycomb-Associated Non-Coding RNAs.
MA557742 - JP 2018138019-A/129668: Polycomb-Associated Non-Coding RNAs.
MA557740 - JP 2018138019-A/129666: Polycomb-Associated Non-Coding RNAs.
MA557739 - JP 2018138019-A/129665: Polycomb-Associated Non-Coding RNAs.
MA557738 - JP 2018138019-A/129664: Polycomb-Associated Non-Coding RNAs.
MA557736 - JP 2018138019-A/129662: Polycomb-Associated Non-Coding RNAs.
MA557735 - JP 2018138019-A/129661: Polycomb-Associated Non-Coding RNAs.
MA557734 - JP 2018138019-A/129660: Polycomb-Associated Non-Coding RNAs.
MA557733 - JP 2018138019-A/129659: Polycomb-Associated Non-Coding RNAs.
MA557732 - JP 2018138019-A/129658: Polycomb-Associated Non-Coding RNAs.
MA444310 - JP 2018138019-A/16236: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_calcineurinPathway - Effects of calcineurin in Keratinocyte Differentiation

Reactome (by CSHL, EBI, and GO)

Protein P29966 (Reactome details) participates in the following event(s):

R-HSA-399978 Protein kinase C, alpha type phosphorylates MARCKS
R-HSA-399997 Acetylcholine regulates insulin secretion
R-HSA-422356 Regulation of insulin secretion
R-HSA-163685 Energy Metabolism
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: E1P560, ENST00000612661.1, MACS, MARCS_HUMAN, NM_002356, P29966, PRKCSL, Q2LA83, Q5TDB7, uc327odh.1, uc327odh.2
UCSC ID: ENST00000612661.2_6
RefSeq Accession: NM_002356.7
Protein: P29966 (aka MARCS_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.