Human Gene MARK3 (ENST00000429436.7_6) from GENCODE V47lift37
  Description: microtubule affinity regulating kinase 3, transcript variant 1 (from RefSeq NM_001128918.3)
Gencode Transcript: ENST00000429436.7_6
Gencode Gene: ENSG00000075413.19_16
Transcript (Including UTRs)
   Position: hg19 chr14:103,851,752-103,970,168 Size: 118,417 Total Exon Count: 18 Strand: +
Coding Region
   Position: hg19 chr14:103,852,367-103,969,564 Size: 117,198 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:103,851,752-103,970,168)mRNA (may differ from genome)Protein (753 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MARK3_HUMAN
DESCRIPTION: RecName: Full=MAP/microtubule affinity-regulating kinase 3; EC=2.7.11.1; AltName: Full=C-TAK1; Short=cTAK1; AltName: Full=Cdc25C-associated protein kinase 1; AltName: Full=ELKL motif kinase 2; Short=EMK-2; AltName: Full=Protein kinase STK10; AltName: Full=Ser/Thr protein kinase PAR-1; Short=Par-1a; AltName: Full=Serine/threonine-protein kinase p78;
FUNCTION: Involved in the specific phosphorylation of microtubule- associated proteins for tau, MAP2 and MAP4. Phosphorylates CDC25C on 'Ser-216'. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by phosphorylation on Thr-211. Inhibited by phosphorylation on Thr-564.
INTERACTION: Q8WXK3:ASB13; NbExp=2; IntAct=EBI-707595, EBI-707573; Q15560:TCEA2; NbExp=2; IntAct=EBI-707595, EBI-710310; P31946:YWHAB; NbExp=2; IntAct=EBI-707595, EBI-359815; P61981:YWHAG; NbExp=2; IntAct=EBI-707595, EBI-359832; Q04917:YWHAH; NbExp=4; IntAct=EBI-707595, EBI-306940; P63104:YWHAZ; NbExp=6; IntAct=EBI-707595, EBI-347088;
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Phosphorylated at Thr-211 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Phosphorylation at Thr-564 by PRKCZ/aPKC inhibits the kinase activity.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
SIMILARITY: Contains 1 KA1 (kinase-associated) domain.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 UBA domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MARK3
Diseases sorted by gene-association score: brain stem astrocytic neoplasm (16), pilocytic astrocytoma of cerebellum (11), cerebellar astrocytoma (9), central hypoventilation syndrome, congenital (5), muenke syndrome (4)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 40.05 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 942.41 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -331.00615-0.538 Picture PostScript Text
3' UTR -143.30604-0.237 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001772 - Kinase-assoc_KA1
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS
IPR015940 - UBA/transl_elong_EF1B_N_euk

Pfam Domains:
PF00069 - Protein kinase domain
PF00627 - UBA/TS-N domain
PF02149 - Kinase associated domain 1
PF07714 - Protein tyrosine and serine/threonine kinase
PF14531 - Kinase-like

SCOP Domains:
46934 - UBA-like
103243 - KA1-like
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2QNJ - X-ray MuPIT 3FE3 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P27448
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0000165 MAPK cascade
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0032092 positive regulation of protein binding
GO:0035331 negative regulation of hippo signaling
GO:0036289 peptidyl-serine autophosphorylation

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BX161395 - human full-length cDNA clone CS0DC011YL17 of Neuroblastoma of Homo sapiens (human).
AF159295 - Homo sapiens serine/threonine protein kinase Kp78 splice variant CTAK75a mRNA, complete cds.
AF465413 - Homo sapiens MAP/microtubule affinity-regulating kinase 3 long isoform (MARK3) mRNA, complete cds; alternatively spliced.
BC024773 - Homo sapiens MAP/microtubule affinity-regulating kinase 3, mRNA (cDNA clone MGC:29880 IMAGE:5138958), complete cds.
U64205 - Homo sapiens Cdc25C associated protein kinase C-TAK1 mRNA, complete cds.
M80359 - Human protein p78 mRNA, complete cds.
AK307448 - Homo sapiens cDNA, FLJ97396.
AF387637 - Homo sapiens Ser/Thr protein kinase PAR-1A mRNA, complete cds.
AB528429 - Synthetic construct DNA, clone: pF1KB5786, Homo sapiens MARK3 gene for MAP/microtubule affinity-regulating kinase 3, without stop codon, in Flexi system.
DQ891715 - Synthetic construct clone IMAGE:100004345; FLH179356.01X; RZPDo839F07130D MAP/microtubule affinity-regulating kinase 3 (MARK3) gene, encodes complete protein.
DQ894892 - Synthetic construct Homo sapiens clone IMAGE:100009352; FLH179352.01L; RZPDo839F07129D MAP/microtubule affinity-regulating kinase 3 (MARK3) gene, encodes complete protein.
KJ897166 - Synthetic construct Homo sapiens clone ccsbBroadEn_06560 MARK3 gene, encodes complete protein.
KJ905226 - Synthetic construct Homo sapiens clone ccsbBroadEn_14693 MARK3 gene, encodes complete protein.
AK124796 - Homo sapiens cDNA FLJ42806 fis, clone BRCAN2004355.
AK296643 - Homo sapiens cDNA FLJ58507 complete cds, highly similar to MAP/microtubule affinity-regulating kinase 3 (EC 2.7.11.1).
AF170723 - Homo sapiens protein kinase STK10 mRNA, partial cds.
BX248250 - human full-length cDNA clone CS0DE006YM09 of Placenta of Homo sapiens (human).
JD336762 - Sequence 317786 from Patent EP1572962.
JD149054 - Sequence 130078 from Patent EP1572962.
JD408210 - Sequence 389234 from Patent EP1572962.
JD445569 - Sequence 426593 from Patent EP1572962.
JD170228 - Sequence 151252 from Patent EP1572962.
JD217417 - Sequence 198441 from Patent EP1572962.
JD178881 - Sequence 159905 from Patent EP1572962.
JD404502 - Sequence 385526 from Patent EP1572962.
JD458347 - Sequence 439371 from Patent EP1572962.
JD129192 - Sequence 110216 from Patent EP1572962.
JD535992 - Sequence 517016 from Patent EP1572962.
CU692932 - Synthetic construct Homo sapiens gateway clone IMAGE:100017468 5' read MARK3 mRNA.
AK023963 - Homo sapiens cDNA FLJ13901 fis, clone THYRO1001772.
M83780 - Human serine/threonine kinase (SLK) mRNA.
DQ368336 - Homo sapiens MAP/microtubule affinity-regulating kinase 3 (MARK3) mRNA, partial cds.
AB209542 - Homo sapiens mRNA for MAP/microtubule affinity-regulating kinase 3 variant protein.
AK124459 - Homo sapiens cDNA FLJ42468 fis, clone BRACE2030341, highly similar to Homo sapiens protein kinase STK10 mRNA.
AK056714 - Homo sapiens cDNA FLJ32152 fis, clone PLACE5000178, highly similar to PUTATIVE SERINE/THREONINE-PROTEIN KINASE P78 (EC 2.7.1.-).
AK055814 - Homo sapiens cDNA FLJ31252 fis, clone KIDNE2005424, highly similar to PUTATIVE SERINE/THREONINE-PROTEIN KINASE P78 (EC 2.7.1.-).
JD399597 - Sequence 380621 from Patent EP1572962.
JD539326 - Sequence 520350 from Patent EP1572962.
JD239089 - Sequence 220113 from Patent EP1572962.
JD109495 - Sequence 90519 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P27448 (Reactome details) participates in the following event(s):

R-HSA-5672948 MARK3 phosphorylates KSR1
R-HSA-5674019 BRAP binds KSR1:MARK3
R-HSA-5672958 YWHAB dimer dissociates from KSR1:MARK3
R-HSA-5672954 p-S311,S406 KSR1:MARK3 binds YWHAB dimer
R-HSA-5672957 PP2A dephosphorylates KSR1
R-HSA-5672972 MAP2Ks and MAPKs bind to the activated RAF complex
R-HSA-6802912 High kinase activity BRAF mutants bind MAP2Ks and MAPKs
R-HSA-6802914 RAS:GTP:moderate kinase activity p-RAF complexes bind MAP2Ks and MAPKs
R-HSA-6802925 Mutant RAS:p-RAF complexes bind MAP2Ks and MAPKs
R-HSA-6802934 p-BRAF and RAF fusion dimers bind MAP2Ks and MAPKs
R-HSA-6802942 RAS:GTP:p-RAF complexes paradoxically bind MAP2Ks and MAPKs
R-HSA-5672980 Dissociation of RAS:RAF complex
R-HSA-6802932 Dissociation of BRAF/RAF fusion complex
R-HSA-6803227 Dissociation of high activity BRAF complexes
R-HSA-6803230 Dissociation of moderate activity BRAF complexes
R-HSA-6803233 Dissociation of oncogenic RAS:RAF complex
R-HSA-6803234 Dissociation of paradoxically activated RAS:BRAF complexes
R-HSA-5672966 RAS:GTP:'activator' RAF homo/heterodimerizes with other RAF monomers
R-HSA-5672978 RAF phosphorylates MAP2K dimer
R-HSA-5672973 MAP2Ks phosphorylate MAPKs
R-HSA-6802918 Activated MAP2Ks phosphorylate MAPKs downstream of inactive BRAF mutants
R-HSA-6802943 RAS:GTP:inactive p-RAF complexes phosphorylate MAP2Ks
R-HSA-6802919 RAS:GTP:moderate kinase activity p-RAF complexes phosphorylate MAP2Ks
R-HSA-6802921 Activated MAP2Ks phosphorylate MAPKs downstream of moderate kinase activity BRAF mutants
R-HSA-6802911 High kinase activity BRAF complexes phosphorylate MAP2Ks
R-HSA-6802910 Activated MAP2Ks phosphorylate MAPKs downstream of high kinase activity BRAF mutants
R-HSA-6802926 Mutant RAS:p-RAF complexes phosphorylate MAP2Ks
R-HSA-6802922 Activated MAP2Ks phosphorylate MAPKs downstream of oncogenic RAS
R-HSA-6802933 p-BRAF and RAF fusion dimers phosphorylate MAP2Ks
R-HSA-6802935 MAPKs are phosphorylated downstream of BRAF and RAF fusion dimers
R-HSA-5672969 Phosphorylation of RAF
R-HSA-5673000 RAF activation
R-HSA-5675221 Negative regulation of MAPK pathway
R-HSA-5673001 RAF/MAP kinase cascade
R-HSA-5674135 MAP2K and MAPK activation
R-HSA-6802948 Signaling by high-kinase activity BRAF mutants
R-HSA-6802946 Signaling by moderate kinase activity BRAF mutants
R-HSA-6802949 Signaling by RAS mutants
R-HSA-6802952 Signaling by BRAF and RAF fusions
R-HSA-6802955 Paradoxical activation of RAF signaling by kinase inactive BRAF
R-HSA-5684996 MAPK1/MAPK3 signaling
R-HSA-6802957 Oncogenic MAPK signaling
R-HSA-5683057 MAPK family signaling cascades
R-HSA-5663202 Diseases of signal transduction
R-HSA-162582 Signal Transduction
R-HSA-1643685 Disease

-  Other Names for This Gene
  Alternate Gene Symbols: A0A0A0MQR8, A0A0A0MST9, A0A0A0MT23, CTAK1, EMK2, ENST00000429436.1, ENST00000429436.2, ENST00000429436.3, ENST00000429436.4, ENST00000429436.5, ENST00000429436.6, MARK3_HUMAN, NM_001128918, O60219, P27448, Q86TT8, Q8TB41, Q8WX83, Q96RG1, Q9UMY9, Q9UN34, uc319yiv.1, uc319yiv.2
UCSC ID: ENST00000429436.7_6
RefSeq Accession: NM_001128918.3
Protein: P27448 (aka MARK3_HUMAN or MRK3_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.