Human Gene MARK4 (ENST00000262891.9_5) from GENCODE V47lift37
  Description: microtubule affinity regulating kinase 4, transcript variant 1 (from RefSeq NM_001199867.2)
Gencode Transcript: ENST00000262891.9_5
Gencode Gene: ENSG00000007047.16_14
Transcript (Including UTRs)
   Position: hg19 chr19:45,754,529-45,808,542 Size: 54,014 Total Exon Count: 17 Strand: +
Coding Region
   Position: hg19 chr19:45,754,847-45,805,968 Size: 51,122 Coding Exon Count: 17 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:45,754,529-45,808,542)mRNA (may differ from genome)Protein (752 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MARK4_HUMAN
DESCRIPTION: RecName: Full=MAP/microtubule affinity-regulating kinase 4; EC=2.7.11.1; AltName: Full=MAP/microtubule affinity-regulating kinase-like 1;
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by phosphorylation on Thr-214.
INTERACTION: P60953:CDC42; NbExp=2; IntAct=EBI-302319, EBI-81752; Q9BYG4:PARD6G; NbExp=2; IntAct=EBI-302319, EBI-295417; Q8ND90:PNMA1; NbExp=2; IntAct=EBI-302319, EBI-302345; P41743:PRKCI; NbExp=2; IntAct=EBI-302319, EBI-286199; Q15831:STK11; NbExp=2; IntAct=EBI-302319, EBI-306838; P23258:TUBG1; NbExp=4; IntAct=EBI-302319, EBI-302589; Q04917:YWHAH; NbExp=6; IntAct=EBI-302319, EBI-306940;
TISSUE SPECIFICITY: Ubiquitous. Isoform 2 is brain-specific.
PTM: Ubiquitinated with 'Lys-29'- and 'Lys-33'-linked polyubiquitins which appear to impede LKB1-mediated phosphorylation. Deubiquitinated by USP9X.
PTM: Phosphorylated at Thr-214 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
SIMILARITY: Contains 1 KA1 (kinase-associated) domain.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 UBA domain.
SEQUENCE CAUTION: Sequence=BAB47489.1; Type=Erroneous initiation; Sequence=BAB55238.1; Type=Erroneous initiation;
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/MARK4ID419.html";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MARK4
Diseases sorted by gene-association score: insomnia, fatal familial (7)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 27.47 RPKM in Testis
Total median expression: 480.94 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -149.30318-0.469 Picture PostScript Text
3' UTR -1003.602574-0.390 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001772 - Kinase-assoc_KA1
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS
IPR000449 - UBA/transl_elong_EF1B_N
IPR015940 - UBA/transl_elong_EF1B_N_euk

Pfam Domains:
PF00069 - Protein kinase domain
PF00627 - UBA/TS-N domain
PF02149 - Kinase associated domain 1
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
46934 - UBA-like
103243 - KA1-like
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q96L34
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008017 microtubule binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0043015 gamma-tubulin binding
GO:0043130 ubiquitin binding
GO:0050321 tau-protein kinase activity

Biological Process:
GO:0000226 microtubule cytoskeleton organization
GO:0001578 microtubule bundle formation
GO:0006468 protein phosphorylation
GO:0007049 cell cycle
GO:0007399 nervous system development
GO:0016310 phosphorylation
GO:0030030 cell projection organization
GO:0043068 positive regulation of programmed cell death
GO:0051301 cell division
GO:0097711 ciliary basal body docking

Cellular Component:
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0015630 microtubule cytoskeleton
GO:0036064 ciliary basal body
GO:0042995 cell projection
GO:0043005 neuron projection


-  Descriptions from all associated GenBank mRNAs
  AB049127 - Homo sapiens MARKL1 mRNA for MAP/microtubule affinity-regulating kinase like 1, complete cds.
AB088047 - Homo sapiens MARKL1L mRNA for microtubule affinity-regulating kinase-like1, complete cds.
JD476273 - Sequence 457297 from Patent EP1572962.
JD129819 - Sequence 110843 from Patent EP1572962.
BC071948 - Homo sapiens MAP/microtubule affinity-regulating kinase 4, mRNA (cDNA clone IMAGE:6579540), complete cds.
JD116760 - Sequence 97784 from Patent EP1572962.
JD399333 - Sequence 380357 from Patent EP1572962.
AK075272 - Homo sapiens cDNA FLJ90791 fis, clone THYRO1001593, moderately similar to PUTATIVE SERINE/THREONINE-PROTEIN KINASE P78 (EC 2.7.1.-).
JD476111 - Sequence 457135 from Patent EP1572962.
AY057448 - Homo sapiens MARK4 serine/threonine protein kinase mRNA, complete cds.
AB058763 - Homo sapiens KIAA1860 mRNA for KIAA1860 protein.
KJ903010 - Synthetic construct Homo sapiens clone ccsbBroadEn_12404 MARK4 gene, encodes complete protein.
AB384310 - Synthetic construct DNA, clone: pF1KSDA1860, Homo sapiens MARK4 gene for MAP/microtubule affinity-regulating kinase 4, complete cds, without stop codon, in Flexi system.
BC166620 - Synthetic construct Homo sapiens clone IMAGE:100066346, MGC:195483 MAP/microtubule affinity-regulating kinase 4 (MARK4) mRNA, encodes complete protein.
AY120867 - Homo sapiens MAP/microtubule affinity-regulating kinase-like 1 (MARK4) mRNA, MARK4-L allele, complete cds; alternatively spliced.
AK074578 - Homo sapiens cDNA FLJ90097 fis, clone HEMBA1005929, weakly similar to H.sapiens mRNA for serine/threonine protein kinase EMK.
AK027619 - Homo sapiens cDNA FLJ14713 fis, clone NT2RP3000845, moderately similar to PUTATIVE SERINE/THREONINE-PROTEIN KINASE P78 (EC 2.7.1.-).
GQ901019 - Homo sapiens clone HEL-T-131 epididymis secretory sperm binding protein mRNA, complete cds.
AB073663 - Homo sapiens primary neuroblastoma cDNA, clone:Nbla00650, full insert sequence.
BC009049 - Homo sapiens MAP/microtubule affinity-regulating kinase 4, mRNA (cDNA clone IMAGE:3874322), complete cds.
JD199104 - Sequence 180128 from Patent EP1572962.
KJ903009 - Synthetic construct Homo sapiens clone ccsbBroadEn_12403 MARK4 gene, encodes complete protein.
JD078815 - Sequence 59839 from Patent EP1572962.
JD522564 - Sequence 503588 from Patent EP1572962.
JD184801 - Sequence 165825 from Patent EP1572962.
JD477758 - Sequence 458782 from Patent EP1572962.
JD403381 - Sequence 384405 from Patent EP1572962.
JD060650 - Sequence 41674 from Patent EP1572962.
JD413491 - Sequence 394515 from Patent EP1572962.
JD036877 - Sequence 17901 from Patent EP1572962.
JD224103 - Sequence 205127 from Patent EP1572962.
JD502964 - Sequence 483988 from Patent EP1572962.
JD256114 - Sequence 237138 from Patent EP1572962.
JD126521 - Sequence 107545 from Patent EP1572962.
JD298015 - Sequence 279039 from Patent EP1572962.
JD449612 - Sequence 430636 from Patent EP1572962.
JD189368 - Sequence 170392 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96L34 (Reactome details) participates in the following event(s):

R-HSA-5626699 MARK4 binds ODF2 in the centriole
R-HSA-5626681 Recruitment of transition zone proteins
R-HSA-5626227 CP110 and CEP97 dissociate from the centriole
R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body
R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-5617833 Cilium Assembly
R-HSA-1852241 Organelle biogenesis and maintenance

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000262891.1, ENST00000262891.2, ENST00000262891.3, ENST00000262891.4, ENST00000262891.5, ENST00000262891.6, ENST00000262891.7, ENST00000262891.8, KIAA1860 , MARK4 , MARK4_HUMAN, MARKL1 , NM_001199867, Q8NG37, Q96JG7, Q96L34, Q96SQ2, Q9BYD8, uc317hek.1, uc317hek.2
UCSC ID: ENST00000262891.9_5
RefSeq Accession: NM_001199867.2
Protein: Q96L34 (aka MARK4_HUMAN or MRK4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.