Human Gene MAT2A (ENST00000306434.8_4) from GENCODE V47lift37
  Description: methionine adenosyltransferase 2A (from RefSeq NM_005911.6)
Gencode Transcript: ENST00000306434.8_4
Gencode Gene: ENSG00000168906.13_7
Transcript (Including UTRs)
   Position: hg19 chr2:85,766,291-85,772,404 Size: 6,114 Total Exon Count: 9 Strand: +
Coding Region
   Position: hg19 chr2:85,766,411-85,770,895 Size: 4,485 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesGeneReviewsModel Information
Methods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:85,766,291-85,772,404)mRNA (may differ from genome)Protein (395 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: METK2_HUMAN
DESCRIPTION: RecName: Full=S-adenosylmethionine synthase isoform type-2; Short=AdoMet synthase 2; EC=2.5.1.6; AltName: Full=Methionine adenosyltransferase 2; Short=MAT 2; AltName: Full=Methionine adenosyltransferase II; Short=MAT-II;
FUNCTION: Catalyzes the formation of S-adenosylmethionine from methionine and ATP.
CATALYTIC ACTIVITY: ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine.
COFACTOR: Binds 2 divalent ions per subunit. Magnesium or cobalt (By similarity).
COFACTOR: Binds 1 potassium ion per subunit (By similarity).
PATHWAY: Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
SUBUNIT: Heterotetramer composed of 2 catalytic alpha subunits (alpha and alpha') (MAT2A) and 1 copy of beta subunit (MAT2B).
PTM: The alpha' subunit is a post-translationally modified version of MAT2A.
SIMILARITY: Belongs to the AdoMet synthase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MAT2A
Diseases sorted by gene-association score: acute maxillary sinusitis (16), glycine n-methyltransferase deficiency (9), hypermethioninemia (6)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 153.80 RPKM in Thyroid
Total median expression: 2920.09 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -31.00120-0.258 Picture PostScript Text
3' UTR -422.401509-0.280 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR022631 - ADOMET_SYNTHASE_CS
IPR022630 - S-AdoMet_synt_C
IPR022629 - S-AdoMet_synt_central
IPR022628 - S-AdoMet_synt_N
IPR002133 - S-AdoMet_synthetase
IPR022636 - S-AdoMet_synthetase_sfam

Pfam Domains:
PF00438 - S-adenosylmethionine synthetase, N-terminal domain
PF02772 - S-adenosylmethionine synthetase, central domain
PF02773 - S-adenosylmethionine synthetase, C-terminal domain

SCOP Domains:
49785 - Galactose-binding domain-like
55973 - S-adenosylmethionine synthetase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2P02 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P31153
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004478 methionine adenosyltransferase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016740 transferase activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006556 S-adenosylmethionine biosynthetic process
GO:0006730 one-carbon metabolic process
GO:0034214 protein hexamerization
GO:0051291 protein heterooligomerization
GO:1990830 cellular response to leukemia inhibitory factor

Cellular Component:
GO:0005829 cytosol
GO:0048269 methionine adenosyltransferase complex


-  Descriptions from all associated GenBank mRNAs
  AK294208 - Homo sapiens cDNA FLJ52225 complete cds, moderately similar to S-adenosylmethionine synthetase isoform type-2 (EC 2.5.1.6).
HM005434 - Homo sapiens clone HTL-T-121 testicular tissue protein Li 121 mRNA, complete cds.
AK311612 - Homo sapiens cDNA, FLJ18654.
AK308591 - Homo sapiens cDNA, FLJ98632.
AK297758 - Homo sapiens cDNA FLJ50997 complete cds, highly similar to S-adenosylmethionine synthetase isoform type-2 (EC 2.5.1.6).
AK293841 - Homo sapiens cDNA FLJ56505 complete cds, highly similar to S-adenosylmethionine synthetase isoform type-2 (EC 2.5.1.6).
AK297797 - Homo sapiens cDNA FLJ51001 complete cds, highly similar to S-adenosylmethionine synthetase isoform type-2 (EC 2.5.1.6).
AK311142 - Homo sapiens cDNA, FLJ18184.
AK303877 - Homo sapiens cDNA FLJ51180 complete cds, moderately similar to S-adenosylmethionine synthetase isoform type-2 (EC 2.5.1.6).
AK291126 - Homo sapiens cDNA FLJ78446 complete cds, highly similar to Homo sapiens methionine adenosyltransferase II, alpha (MAT2A), mRNA.
BC001686 - Homo sapiens methionine adenosyltransferase II, alpha, mRNA (cDNA clone MGC:2907 IMAGE:3010820), complete cds.
BC001854 - Homo sapiens methionine adenosyltransferase II, alpha, mRNA (cDNA clone MGC:4537 IMAGE:3010820), complete cds.
DL491507 - Novel nucleic acids.
DL490116 - Novel nucleic acids.
X68836 - H.sapiens mRNA for S-adenosylmethionine synthetase.
DQ083239 - Homo sapiens methionine adenosyltransferase II (MAT2A) mRNA, complete cds.
JD097604 - Sequence 78628 from Patent EP1572962.
CU674832 - Synthetic construct Homo sapiens gateway clone IMAGE:100019218 5' read MAT2A mRNA.
AB529143 - Synthetic construct DNA, clone: pF1KB5179, Homo sapiens MAT2A gene for methionine adenosyltransferase II, alpha, without stop codon, in Flexi system.
JF432119 - Synthetic construct Homo sapiens clone IMAGE:100073258 methionine adenosyltransferase II, alpha (MAT2A) gene, encodes complete protein.
KJ891581 - Synthetic construct Homo sapiens clone ccsbBroadEn_00975 MAT2A gene, encodes complete protein.
AK308758 - Homo sapiens cDNA, FLJ98799.
AK316411 - Homo sapiens cDNA, FLJ79310 complete cds, highly similar to S-adenosylmethionine synthetase isoform type-2 (EC 2.5.1.6).
AK309338 - Homo sapiens cDNA, FLJ99379.
DQ579887 - Homo sapiens piRNA piR-47999, complete sequence.
JD020761 - Sequence 1785 from Patent EP1572962.
JD547449 - Sequence 528473 from Patent EP1572962.
JD354888 - Sequence 335912 from Patent EP1572962.
JD091792 - Sequence 72816 from Patent EP1572962.
JD402314 - Sequence 383338 from Patent EP1572962.
JD546747 - Sequence 527771 from Patent EP1572962.
JD056958 - Sequence 37982 from Patent EP1572962.
JD352758 - Sequence 333782 from Patent EP1572962.
JD292953 - Sequence 273977 from Patent EP1572962.
JD503432 - Sequence 484456 from Patent EP1572962.
JD452486 - Sequence 433510 from Patent EP1572962.
JD334090 - Sequence 315114 from Patent EP1572962.
JD040169 - Sequence 21193 from Patent EP1572962.
JD533596 - Sequence 514620 from Patent EP1572962.
JD447491 - Sequence 428515 from Patent EP1572962.
JD345314 - Sequence 326338 from Patent EP1572962.
JD114879 - Sequence 95903 from Patent EP1572962.
JD363312 - Sequence 344336 from Patent EP1572962.
JD316853 - Sequence 297877 from Patent EP1572962.
JD377756 - Sequence 358780 from Patent EP1572962.
JD403614 - Sequence 384638 from Patent EP1572962.
JD241826 - Sequence 222850 from Patent EP1572962.
JD220860 - Sequence 201884 from Patent EP1572962.
JD411299 - Sequence 392323 from Patent EP1572962.
JD407872 - Sequence 388896 from Patent EP1572962.
JD159180 - Sequence 140204 from Patent EP1572962.
JD209709 - Sequence 190733 from Patent EP1572962.
JD491257 - Sequence 472281 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
METHIONINE-DEG1-PWY - L-methionine degradation
PWY-5328 - superpathway of methionine degradation
PWY-6292 - L-cysteine biosynthesis
PWY-7527 - L-methionine salvage cycle
SAM-PWY - S-adenosyl-L-methionine biosynthesis

Reactome (by CSHL, EBI, and GO)

Protein P31153 (Reactome details) participates in the following event(s):

R-HSA-5603114 MAT2B:MAT2A:K+:2Mg2+ transfers Ado from ATP to L-Met
R-HSA-156581 Methylation
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: A8K511, AMS2, B4DN45, D6W5L1, ENST00000306434.1, ENST00000306434.2, ENST00000306434.3, ENST00000306434.4, ENST00000306434.5, ENST00000306434.6, ENST00000306434.7, MATA2, METK2_HUMAN, NM_005911, P31153, Q53SP5, uc317nvu.1, uc317nvu.2
UCSC ID: ENST00000306434.8_4
RefSeq Accession: NM_005911.6
Protein: P31153 (aka METK2_HUMAN)

-  GeneReviews for This Gene
  GeneReviews article(s) related to gene MAT2A:
taa (Heritable Thoracic Aortic Disease Overview)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.