Human Gene MATK (ENST00000310132.11_12) from GENCODE V47lift37
  Description: megakaryocyte-associated tyrosine kinase, transcript variant 1 (from RefSeq NM_139355.3)
Gencode Transcript: ENST00000310132.11_12
Gencode Gene: ENSG00000007264.15_15
Transcript (Including UTRs)
   Position: hg19 chr19:3,777,971-3,786,379 Size: 8,409 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr19:3,778,181-3,785,133 Size: 6,953 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:3,777,971-3,786,379)mRNA (may differ from genome)Protein (507 aa)
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-  Comments and Description Text from UniProtKB
  ID: MATK_HUMAN
DESCRIPTION: RecName: Full=Megakaryocyte-associated tyrosine-protein kinase; EC=2.7.10.2; AltName: Full=CSK homologous kinase; Short=CHK; AltName: Full=Hematopoietic consensus tyrosine-lacking kinase; AltName: Full=Protein kinase HYL; AltName: Full=Tyrosine-protein kinase CTK;
FUNCTION: Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT: Interacts with KIT.
SUBCELLULAR LOCATION: Cytoplasm. Membrane. Note=In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.
TISSUE SPECIFICITY: Expressed in various myeloid cell lines, detected in brain and lung.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.
SIMILARITY: Contains 1 protein kinase domain.
SIMILARITY: Contains 1 SH2 domain.
SIMILARITY: Contains 1 SH3 domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MATK
Diseases sorted by gene-association score: regular astigmatism (1), endocardium disease (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 17.36 RPKM in Brain - Cortex
Total median expression: 143.12 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -169.40364-0.465 Picture PostScript Text
3' UTR -88.30210-0.420 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR001245 - Ser-Thr/Tyr_kinase_cat_dom
IPR000980 - SH2
IPR001452 - SH3_domain
IPR008266 - Tyr_kinase_AS
IPR020635 - Tyr_kinase_cat_dom

Pfam Domains:
PF00017 - SH2 domain
PF00018 - SH3 domain
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
50044 - SH3-domain
56112 - Protein kinase-like (PK-like)
55550 - SH2 domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1JWO - X-ray MuPIT 1X6G - NMR MuPIT 3US4 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P42679
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004713 protein tyrosine kinase activity
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005102 receptor binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
GO:0007498 mesoderm development
GO:0008283 cell proliferation
GO:0008284 positive regulation of cell proliferation
GO:0016310 phosphorylation
GO:0030154 cell differentiation
GO:0038083 peptidyl-tyrosine autophosphorylation
GO:0038128 ERBB2 signaling pathway
GO:0045087 innate immune response

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane
GO:0031234 extrinsic component of cytoplasmic side of plasma membrane


-  Descriptions from all associated GenBank mRNAs
  AK055395 - Homo sapiens cDNA FLJ30833 fis, clone FEBRA2002027, highly similar to Megakaryocyte-associated tyrosine-protein kinase (EC 2.7.10.2).
X77278 - H.sapiens HYL tyrosine kinase mRNA.
E10345 - cDNA encoding human novel tyrosine kinase.
AK225834 - Homo sapiens mRNA for megakaryocyte-associated tyrosine kinase isoform a variant, clone: FCC127E02.
AL137754 - Homo sapiens mRNA; cDNA DKFZp434N1212 (from clone DKFZp434N1212).
AB593100 - Homo sapiens MATK mRNA for megakaryocyte-associated tyrosine-protein kinase, complete cds, clone: HP06805-RBd01B07.
BC000114 - Homo sapiens megakaryocyte-associated tyrosine kinase, mRNA (cDNA clone MGC:1708 IMAGE:3350365), complete cds.
BC003109 - Homo sapiens megakaryocyte-associated tyrosine kinase, mRNA (cDNA clone MGC:2101 IMAGE:3357648), complete cds.
S71669 - lsk=leukocyte carboxyl-terminal src kinase related gene [human, peripheral T cells, mRNA, 1744 nt].
L18974 - Human tyrosine kinase (MATK) mRNA, complete cds.
JD270302 - Sequence 251326 from Patent EP1572962.
JD367416 - Sequence 348440 from Patent EP1572962.
JD115960 - Sequence 96984 from Patent EP1572962.
JD544076 - Sequence 525100 from Patent EP1572962.
AK315646 - Homo sapiens cDNA, FLJ96734, highly similar to Homo sapiens megakaryocyte-associated tyrosine kinase (MATK), transcript variant 1, mRNA.
AB590864 - Synthetic construct DNA, clone: pFN21AB0459, Homo sapiens MATK gene for megakaryocyte-associated tyrosine kinase, without stop codon, in Flexi system.
JD532029 - Sequence 513053 from Patent EP1572962.
JD318961 - Sequence 299985 from Patent EP1572962.
JD129332 - Sequence 110356 from Patent EP1572962.
JD320928 - Sequence 301952 from Patent EP1572962.
JD487427 - Sequence 468451 from Patent EP1572962.
JD485643 - Sequence 466667 from Patent EP1572962.
JD215153 - Sequence 196177 from Patent EP1572962.
JD095637 - Sequence 76661 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P42679 (Reactome details) participates in the following event(s):

R-HSA-1963563 MATK (CSK homologous kinase) binds phosphorylated ERBB2
R-HSA-8863795 Downregulation of ERBB2 signaling
R-HSA-1227986 Signaling by ERBB2
R-HSA-9006934 Signaling by Receptor Tyrosine Kinases
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B3KNZ9, CTK, ENST00000310132.1, ENST00000310132.10, ENST00000310132.2, ENST00000310132.3, ENST00000310132.4, ENST00000310132.5, ENST00000310132.6, ENST00000310132.7, ENST00000310132.8, ENST00000310132.9, HYL, MATK_HUMAN, NM_139355, P42679, Q9NST8, uc317ort.1, uc317ort.2
UCSC ID: ENST00000310132.11_12
RefSeq Accession: NM_139355.3
Protein: P42679 (aka MATK_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.