ID:MCM3_HUMAN DESCRIPTION: RecName: Full=DNA replication licensing factor MCM3; EC=3.6.4.12; AltName: Full=DNA polymerase alpha holoenzyme-associated protein P1; AltName: Full=P1-MCM3; AltName: Full=RLF subunit beta; AltName: Full=p102; FUNCTION: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. SUBUNIT: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. INTERACTION: P62805:HIST2H4B; NbExp=2; IntAct=EBI-355153, EBI-302023; P49736:MCM2; NbExp=4; IntAct=EBI-355153, EBI-374819; P33992:MCM5; NbExp=3; IntAct=EBI-355153, EBI-359410; Q14566:MCM6; NbExp=2; IntAct=EBI-355153, EBI-374900; Q13416:ORC2; NbExp=2; IntAct=EBI-355153, EBI-374957; SUBCELLULAR LOCATION: Nucleus. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. MISCELLANEOUS: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. The MCM2-7 hexamer is the proposed physiological active complex. SIMILARITY: Belongs to the MCM family. SIMILARITY: Contains 1 MCM domain. WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mcm3/";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P25205
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AY032603 - Homo sapiens cervical cancer proto-oncogene 5 (HCC5) mRNA, complete cds. BC001626 - Homo sapiens minichromosome maintenance complex component 3, mRNA (cDNA clone MGC:1157 IMAGE:3542098), complete cds. AK222586 - Homo sapiens mRNA for minichromosome maintenance protein 3 variant, clone: CAS04981. X62153 - H.sapiens mRNA for P1 protein (P1.h). D38073 - Homo sapiens mRNA for hRlf beta subunit (p102 protein), complete cds. BC003509 - Homo sapiens minichromosome maintenance complex component 3, mRNA (cDNA clone MGC:10548 IMAGE:3610786), complete cds. JD330628 - Sequence 311652 from Patent EP1572962. JD294781 - Sequence 275805 from Patent EP1572962. JD202296 - Sequence 183320 from Patent EP1572962. JD565845 - Sequence 546869 from Patent EP1572962. AK300754 - Homo sapiens cDNA FLJ50873 complete cds, highly similar to DNA replication licensing factor MCM3. JD544298 - Sequence 525322 from Patent EP1572962. JD111615 - Sequence 92639 from Patent EP1572962. JD532797 - Sequence 513821 from Patent EP1572962. AK301704 - Homo sapiens cDNA FLJ55599 complete cds, highly similar to DNA replication licensing factor MCM3. JD393838 - Sequence 374862 from Patent EP1572962. JD519966 - Sequence 500990 from Patent EP1572962. JD297552 - Sequence 278576 from Patent EP1572962. JD528050 - Sequence 509074 from Patent EP1572962. AK299566 - Homo sapiens cDNA FLJ55751 complete cds, highly similar to DNA replication licensing factor MCM3. JD421783 - Sequence 402807 from Patent EP1572962. AK312928 - Homo sapiens cDNA, FLJ93378, highly similar to Homo sapiens MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) (MCM3), mRNA. KJ891593 - Synthetic construct Homo sapiens clone ccsbBroadEn_00987 MCM3 gene, encodes complete protein. DQ893654 - Synthetic construct clone IMAGE:100006284; FLH188159.01X; RZPDo839H01150D MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) (MCM3) gene, encodes complete protein. DQ895840 - Synthetic construct Homo sapiens clone IMAGE:100010300; FLH188155.01L; RZPDo839H01149D MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) (MCM3) gene, encodes complete protein. KU178170 - Homo sapiens minichromosome maintenance complex component 3 isoform 1 (MCM3) mRNA, partial cds. KU178171 - Homo sapiens minichromosome maintenance complex component 3 isoform 2 (MCM3) mRNA, complete cds, alternatively spliced. AB528319 - Synthetic construct DNA, clone: pF1KB5749, Homo sapiens MCM3 gene for minichromosome maintenance complex component 3, without stop codon, in Flexi system. JD030120 - Sequence 11144 from Patent EP1572962. JD021634 - Sequence 2658 from Patent EP1572962. JD439967 - Sequence 420991 from Patent EP1572962. JD076207 - Sequence 57231 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_mcmPathway - CDK Regulation of DNA Replication
Reactome (by CSHL, EBI, and GO)
Protein P25205 (Reactome details) participates in the following event(s):
R-HSA-69019 Mcm4,6,7 trimer forms and associates with the replication fork R-HSA-68849 Mcm2-7 associates with the Cdt1:CDC6:ORC:origin complex, forming the pre-replicative complex (preRC) R-HSA-68954 Mcm2-7 is phosphorylated by DDK R-HSA-176942 Multiple proteins are localized at replication fork R-HSA-68919 Mcm10 associates with the pre-replicative complex, stabilizing Mcm2-7 R-HSA-68944 Orc1 is phosphorylated by cyclin A/CDK2 R-HSA-169468 MCM2-7 mediated fork unwinding R-HSA-68940 Cdt1 is displaced from the pre-replicative complex. R-HSA-68918 CDK and DDK associate with the Mcm10:pre-replicative complex R-HSA-68917 Cdc45 associates with the pre-replicative complex at the origin R-HSA-68916 DNA Replication Factor A (RPA) associates with the pre-replicative complex at the origin R-HSA-176318 Loading of claspin onto DNA during replication origin firing R-HSA-176298 Activation of claspin R-HSA-69052 Switching of origins to a post-replicative state R-HSA-68867 Assembly of the pre-replicative complex R-HSA-68962 Activation of the pre-replicative complex R-HSA-176974 Unwinding of DNA R-HSA-69239 Synthesis of DNA R-HSA-68949 Orc1 removal from chromatin R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-69190 DNA strand elongation R-HSA-69242 S Phase R-HSA-69306 DNA Replication R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-69278 Cell Cycle (Mitotic) R-HSA-1640170 Cell Cycle R-HSA-176187 Activation of ATR in response to replication stress R-HSA-69481 G2/M Checkpoints R-HSA-69620 Cell Cycle Checkpoints
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
