ID:MCM7_HUMAN DESCRIPTION: RecName: Full=DNA replication licensing factor MCM7; EC=3.6.4.12; AltName: Full=CDC47 homolog; AltName: Full=P1.1-MCM3; FUNCTION: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. SUBUNIT: Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6- MCM4-MCM7-MCM3-MCM5 (By simililarity). Interacts with the ATR- ATRIP complex and with RAD17. Interacts with TIPIN. Interacts with MCMBP. INTERACTION: Q9UL03:INTS6; NbExp=10; IntAct=EBI-355924, EBI-1381827; P49736:MCM2; NbExp=5; IntAct=EBI-355924, EBI-374819; P33991:MCM4; NbExp=6; IntAct=EBI-355924, EBI-374938; Q14566:MCM6; NbExp=4; IntAct=EBI-355924, EBI-374900; P01106:MYC; NbExp=6; IntAct=EBI-355924, EBI-447544; P53350:PLK1; NbExp=4; IntAct=EBI-355924, EBI-476768; Q14683:SMC1A; NbExp=8; IntAct=EBI-355924, EBI-80690; P0CG47:UBB; NbExp=2; IntAct=EBI-355924, EBI-413034; SUBCELLULAR LOCATION: Nucleus (By similarity). PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. MISCELLANEOUS: Early fractionation of eukaryotic MCM proteins yielded a variety of dimeric, trimeric and tetrameric complexes with unclear biological significance. Specifically a MCM467 subcomplex is shown to have in vitro helicase activity which is inhibited by the MCM2 subunit. The MCM2-7 hexamer is the proposed physiological active complex. SIMILARITY: Belongs to the MCM family. SIMILARITY: Contains 1 MCM domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P33993
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0000082 G1/S transition of mitotic cell cycle GO:0006260 DNA replication GO:0006268 DNA unwinding involved in DNA replication GO:0006270 DNA replication initiation GO:0006974 cellular response to DNA damage stimulus GO:0007049 cell cycle GO:0008283 cell proliferation GO:0042325 regulation of phosphorylation GO:0042493 response to drug GO:0071310 cellular response to organic substance GO:0071364 cellular response to epidermal growth factor stimulus GO:0071466 cellular response to xenobiotic stimulus
AK226175 - Homo sapiens mRNA for minichromosome maintenance protein 7 isoform 1 variant, clone: bm04567. AY007130 - Homo sapiens clone CDABP0042 mRNA sequence. BC009398 - Homo sapiens minichromosome maintenance complex component 7, mRNA (cDNA clone MGC:15341 IMAGE:4134871), complete cds. BC013375 - Homo sapiens minichromosome maintenance complex component 7, mRNA (cDNA clone MGC:16436 IMAGE:3946342), complete cds. D28480 - Homo sapiens hMCM2 mRNA, complete cds. X74796 - H.sapiens p85Mcm mRNA. AK055379 - Homo sapiens cDNA FLJ30817 fis, clone FEBRA2001581, highly similar to DNA REPLICATION LICENSING FACTOR MCM7. AK293172 - Homo sapiens cDNA FLJ55929 complete cds, highly similar to DNA replication licensing factor MCM7. D86748 - Homo sapiens mRNA for HsMcm7, complete cds. AK096959 - Homo sapiens cDNA FLJ39640 fis, clone SMINT2003386, highly similar to DNA REPLICATION LICENSING FACTOR MCM7. D55716 - Homo sapiens mRNA for P1cdc47, complete cds. JD392368 - Sequence 373392 from Patent EP1572962. JD335924 - Sequence 316948 from Patent EP1572962. JD077257 - Sequence 58281 from Patent EP1572962. JD254497 - Sequence 235521 from Patent EP1572962. JD085572 - Sequence 66596 from Patent EP1572962. JD433454 - Sequence 414478 from Patent EP1572962. JD206756 - Sequence 187780 from Patent EP1572962. AK314575 - Homo sapiens cDNA, FLJ95407, highly similar to Homo sapiens MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) (MCM7), mRNA. KJ897178 - Synthetic construct Homo sapiens clone ccsbBroadEn_06572 MCM7 gene, encodes complete protein. KJ901567 - Synthetic construct Homo sapiens clone ccsbBroadEn_10961 MCM7 gene, encodes complete protein. DQ895947 - Synthetic construct Homo sapiens clone IMAGE:100010407; FLH194975.01L; RZPDo839H0170D MCM7 minichromosome maintenance deficient 7 (S. cerevisiae) (MCM7) gene, encodes complete protein. EU176445 - Synthetic construct Homo sapiens clone IMAGE:100006521; FLH194982.01X; RZPDo839E08252D minichromosome maintenance complex component 7 (MCM7) gene, encodes complete protein. AB527186 - Synthetic construct DNA, clone: pF1KB5387, Homo sapiens MCM7 gene for minichromosome maintenance complex component 7, without stop codon, in Flexi system. KU178172 - Homo sapiens minichromosome maintenance complex component 7 isoform 1 (MCM7) mRNA, partial cds. KU178173 - Homo sapiens minichromosome maintenance complex component 7 isoform 2 (MCM7) mRNA, complete cds, alternatively spliced. AF279900 - Homo sapiens PNAS-146 mRNA, complete cds. DQ579036 - Homo sapiens piRNA piR-47148, complete sequence. CU680582 - Synthetic construct Homo sapiens gateway clone IMAGE:100020038 5' read MCM7 mRNA. JD191866 - Sequence 172890 from Patent EP1572962. JD072898 - Sequence 53922 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_mcmPathway - CDK Regulation of DNA Replication
Reactome (by CSHL, EBI, and GO)
Protein P33993 (Reactome details) participates in the following event(s):
R-HSA-68849 Mcm2-7 associates with the Cdt1:CDC6:ORC:origin complex, forming the pre-replicative complex (preRC) R-HSA-68954 Mcm2-7 is phosphorylated by DDK R-HSA-69019 Mcm4,6,7 trimer forms and associates with the replication fork R-HSA-176942 Multiple proteins are localized at replication fork R-HSA-68919 Mcm10 associates with the pre-replicative complex, stabilizing Mcm2-7 R-HSA-68944 Orc1 is phosphorylated by cyclin A/CDK2 R-HSA-169468 MCM2-7 mediated fork unwinding R-HSA-68940 Cdt1 is displaced from the pre-replicative complex. R-HSA-68918 CDK and DDK associate with the Mcm10:pre-replicative complex R-HSA-68917 Cdc45 associates with the pre-replicative complex at the origin R-HSA-68916 DNA Replication Factor A (RPA) associates with the pre-replicative complex at the origin R-HSA-176318 Loading of claspin onto DNA during replication origin firing R-HSA-176298 Activation of claspin R-HSA-68867 Assembly of the pre-replicative complex R-HSA-68962 Activation of the pre-replicative complex R-HSA-69052 Switching of origins to a post-replicative state R-HSA-176974 Unwinding of DNA R-HSA-68949 Orc1 removal from chromatin R-HSA-69002 DNA Replication Pre-Initiation R-HSA-69206 G1/S Transition R-HSA-69239 Synthesis of DNA R-HSA-69190 DNA strand elongation R-HSA-68874 M/G1 Transition R-HSA-453279 Mitotic G1-G1/S phases R-HSA-69242 S Phase R-HSA-69306 DNA Replication R-HSA-69278 Cell Cycle (Mitotic) R-HSA-176187 Activation of ATR in response to replication stress R-HSA-1640170 Cell Cycle R-HSA-69481 G2/M Checkpoints R-HSA-69620 Cell Cycle Checkpoints
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
