Human Gene ME1 (ENST00000369705.4_7) from GENCODE V47lift37
  Description: malic enzyme 1 (from RefSeq NM_002395.6)
Gencode Transcript: ENST00000369705.4_7
Gencode Gene: ENSG00000065833.9_9
Transcript (Including UTRs)
   Position: hg19 chr6:83,920,121-84,140,770 Size: 220,650 Total Exon Count: 14 Strand: -
Coding Region
   Position: hg19 chr6:83,921,643-84,140,673 Size: 219,031 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:83,920,121-84,140,770)mRNA (may differ from genome)Protein (572 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MAOX_HUMAN
DESCRIPTION: RecName: Full=NADP-dependent malic enzyme; Short=NADP-ME; EC=1.1.1.40; AltName: Full=Malic enzyme 1;
CATALYTIC ACTIVITY: (S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
CATALYTIC ACTIVITY: Oxaloacetate = pyruvate + CO(2).
COFACTOR: Divalent metal cations. Prefers magnesium or manganese (By similarity).
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in all tissues tested including liver, placenta and white adipose tissue.
SIMILARITY: Belongs to the malic enzymes family.
WEB RESOURCE: Name=SHMPD; Note=The Singapore human mutation and polymorphism database; URL="http://shmpd.bii.a-star.edu.sg/gene.php?genestart=A&genename=ME1";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 39.77 RPKM in Adrenal Gland
Total median expression: 432.92 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -31.9097-0.329 Picture PostScript Text
3' UTR -337.401522-0.222 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015884 - Malic_enzyme_CS
IPR012301 - Malic_N
IPR012302 - Malic_NAD-bd
IPR001891 - Malic_OxRdtase
IPR016040 - NAD(P)-bd_dom

Pfam Domains:
PF00390 - Malic enzyme, N-terminal domain
PF03949 - Malic enzyme, NAD binding domain

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
53223 - Aminoacid dehydrogenase-like, N-terminal domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2AW5 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P48163
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
Gene DetailsGene Details Gene Details Gene Details
Gene SorterGene Sorter Gene Sorter Gene Sorter
 RGD   SGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004470 malic enzyme activity
GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948 oxaloacetate decarboxylase activity
GO:0009055 electron carrier activity
GO:0016491 oxidoreductase activity
GO:0030145 manganese ion binding
GO:0043531 ADP binding
GO:0046872 metal ion binding
GO:0050661 NADP binding
GO:0051287 NAD binding

Biological Process:
GO:0005975 carbohydrate metabolic process
GO:0006090 pyruvate metabolic process
GO:0006108 malate metabolic process
GO:0006741 NADP biosynthetic process
GO:0009725 response to hormone
GO:0009743 response to carbohydrate
GO:0019216 regulation of lipid metabolic process
GO:0022900 electron transport chain
GO:0051262 protein tetramerization
GO:0055114 oxidation-reduction process
GO:1902031 regulation of NADP metabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  LP747427 - Sequence 14 from Patent WO2018009939.
AK289783 - Homo sapiens cDNA FLJ76221 complete cds, highly similar to Homo sapiens malic enzyme 1, NADP(+)-dependent, cytosolic (ME1), mRNA.
U43944 - Human breast cancer cytosolic NADP(+)-dependent malic enzyme mRNA, partial cds.
AK301875 - Homo sapiens cDNA FLJ57648 complete cds, highly similar to NADP-dependent malic enzyme (EC 1.1.1.40).
X77244 - H.sapiens NADP+ dependent cytoplasmic malic enzyme mRNA.
AK223417 - Homo sapiens mRNA for cytosolic malic enzyme 1 variant, clone: FCC109G08.
AK302777 - Homo sapiens cDNA FLJ57677 complete cds, highly similar to NADP-dependent malic enzyme (EC 1.1.1.40).
BC025246 - Homo sapiens malic enzyme 1, NADP(+)-dependent, cytosolic, mRNA (cDNA clone MGC:39115 IMAGE:4870714), complete cds.
L34035 - Homo sapiens NADP-dependent malic enzyme mRNA, complete cds.
DQ892155 - Synthetic construct clone IMAGE:100004785; FLH183523.01X; RZPDo839D07142D malic enzyme 1, NADP(+)-dependent, cytosolic (ME1) gene, encodes complete protein.
EU176687 - Synthetic construct Homo sapiens clone IMAGE:100011462; FLH183522.01L; RZPDo839H03255D malic enzyme 1, NADP(+)-dependent, cytosolic (ME1) gene, encodes complete protein.
AB528430 - Synthetic construct DNA, clone: pF1KB3236, Homo sapiens ME1 gene for malic enzyme 1, NADP(+)-dependent, cytosolic, without stop codon, in Flexi system.
MB486567 - JP 2019531699-A/14: METHODS FOR DIAGNOSING AND TREATING CANCER.
AJ420574 - Homo sapiens mRNA full length insert cDNA clone EUROIMAGE 1644933.
BC017403 - Homo sapiens, clone IMAGE:4290619, mRNA, partial cds.
JD234864 - Sequence 215888 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
MALATE-ASPARTATE-SHUTTLE-PWY - malate-aspartate shuttle
PWY66-398 - TCA cycle
PWY66-399 - gluconeogenesis
PWY66-407 - superpathway of conversion of glucose to acetyl CoA and entry into the TCA cycle

Reactome (by CSHL, EBI, and GO)

Protein P48163 (Reactome details) participates in the following event(s):

R-HSA-9012036 ME1:Mg2+ tetramer oxidatively decarboxylates MAL to PYR
R-HSA-1989781 PPARA activates gene expression
R-HSA-400206 Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)
R-HSA-556833 Metabolism of lipids
R-HSA-1430728 Metabolism
R-HSA-70268 Pyruvate metabolism
R-HSA-71406 Pyruvate metabolism and Citric Acid (TCA) cycle
R-HSA-1428517 The citric acid (TCA) cycle and respiratory electron transport

-  Other Names for This Gene
  Alternate Gene Symbols: B4DZ70, ENST00000369705.1, ENST00000369705.2, ENST00000369705.3, MAOX_HUMAN, ME1 , NM_002395, P48163, Q16797, Q16855, Q53F72, Q5VWA2, Q9BWX8, Q9H1W3, Q9UIY4, uc318hua.1, uc318hua.2
UCSC ID: ENST00000369705.4_7
RefSeq Accession: NM_002395.6
Protein: P48163 (aka MAOX_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.