ID:MED15_HUMAN DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 15; AltName: Full=Activator-recruited cofactor 105 kDa component; Short=ARC105; AltName: Full=CTG repeat protein 7a; AltName: Full=Mediator complex subunit 15; AltName: Full=Positive cofactor 2 glutamine/Q-rich-associated protein; Short=PC2 glutamine/Q-rich-associated protein; AltName: Full=TPA-inducible gene 1 protein; Short=TIG-1; AltName: Full=Trinucleotide repeat-containing gene 7 protein; FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway. SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with SMAD2, SMAD3, SREBF1 and SREBF2. Interacts with WWTR1. Interacts with TRIM11. INTERACTION: P36956-1:SREBF1; NbExp=6; IntAct=EBI-394506, EBI-948328; SUBCELLULAR LOCATION: Cytoplasm. Nucleus. TISSUE SPECIFICITY: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney. INDUCTION: By 12-O-tetradecanoylphorbol-13-acetate (TPA). PTM: Ubiquitinated by TRIM11, leading to proteasomal degradation. POLYMORPHISM: The poly-Gln region from amino acids 235-262 of PCQAP is polymorphic. There are from 15 to 18 repeats in the Italian population. SIMILARITY: Belongs to the Mediator complex subunit 15 family. SEQUENCE CAUTION: Sequence=AAC12944.1; Type=Frameshift; Positions=13, 600, 749; Sequence=BAB85034.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors; Sequence=BAC03446.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96RN5
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0001104 RNA polymerase II transcription cofactor activity GO:0005515 protein binding
Biological Process: GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006357 regulation of transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
