ID:MED8_HUMAN DESCRIPTION: RecName: Full=Mediator of RNA polymerase II transcription subunit 8; AltName: Full=Activator-recruited cofactor 32 kDa component; Short=ARC32; AltName: Full=Mediator complex subunit 8; FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. PATHWAY: Protein modification; protein ubiquitination. SUBUNIT: Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. May be part of a multisubunit E3 ubiquitin-protein ligase complex with the elongin BC complex (TCEB1 and TCEB2), CUL2 and RBX1. INTERACTION: Q15369:TCEB1; NbExp=3; IntAct=EBI-394405, EBI-301231; Q15370:TCEB2; NbExp=5; IntAct=EBI-394405, EBI-301238; SUBCELLULAR LOCATION: Nucleus (Probable). DOMAIN: The elongin BC complex binding domain is also known as BC- box with the consensus [APST]-L-x(3)-C-x(3)-[AILV]. SIMILARITY: Belongs to the Mediator complex subunit 8 family. SEQUENCE CAUTION: Sequence=BG722466; Type=Frameshift; Positions=258;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF10232 - Mediator of RNA polymerase II transcription complex subunit 8
ModBase Predicted Comparative 3D Structure on Q96G25
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Gene Ontology (GO) Annotations with Structured Vocabulary
Molecular Function: GO:0001104 RNA polymerase II transcription cofactor activity GO:0005515 protein binding
Biological Process: GO:0006351 transcription, DNA-templated GO:0006355 regulation of transcription, DNA-templated GO:0006357 regulation of transcription from RNA polymerase II promoter GO:0006367 transcription initiation from RNA polymerase II promoter GO:0016567 protein ubiquitination
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
