Human Gene MIER1 (ENST00000401041.6_6) from GENCODE V47lift37
  Description: MIER1 transcriptional regulator, transcript variant 12 (from RefSeq NM_001350530.2)
Gencode Transcript: ENST00000401041.6_6
Gencode Gene: ENSG00000198160.15_14
Transcript (Including UTRs)
   Position: hg19 chr1:67,390,673-67,454,302 Size: 63,630 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr1:67,390,712-67,450,583 Size: 59,872 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:67,390,673-67,454,302)mRNA (may differ from genome)Protein (565 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MIER1_HUMAN
DESCRIPTION: RecName: Full=Mesoderm induction early response protein 1; Short=Early response 1; Short=Er1; Short=Mi-er1; Short=hMi-er1;
FUNCTION: Transcriptional repressor regulating the expression of a number of genes including SP1 target genes. Probably functions through recruitment of HDAC1 a histone deacetylase involved in chromatin silencing.
SUBUNIT: Interacts with HDAC1.
INTERACTION: Q13547:HDAC1; NbExp=5; IntAct=EBI-3504940, EBI-301834;
SUBCELLULAR LOCATION: Isoform 1: Nucleus.
SUBCELLULAR LOCATION: Isoform 2: Nucleus.
SUBCELLULAR LOCATION: Isoform 3: Nucleus.
SUBCELLULAR LOCATION: Isoform 4: Cytoplasm.
SUBCELLULAR LOCATION: Isoform 5: Cytoplasm.
SUBCELLULAR LOCATION: Isoform 6: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitously expressed, but at very low levels. However, consistent level of expression are observed in heart, testis, thyroid, ovary and adrenal gland. Transcripts are up-regulated in breast carcinoma cell lines and tumor.
SIMILARITY: Contains 1 ELM2 domain.
SIMILARITY: Contains 1 SANT domain.
SEQUENCE CAUTION: Sequence=AAH17423.1; Type=Miscellaneous discrepancy; Note=Wrong choice of CDS. Probable cloning artifact due to reverse transcription from RNA internal poly-A tracts; Sequence=AAM76041.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAM97500.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAM97503.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAM97506.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAC11339.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA. C-terminal is identical to the product of the WLS gene; Sequence=CAD89921.1; Type=Miscellaneous discrepancy; Note=Wrong choice of CDS;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MIER1
Diseases sorted by gene-association score: regular astigmatism (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.52 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 248.01 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -10.2039-0.262 Picture PostScript Text
3' UTR -811.473719-0.218 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000949 - ELM2_dom
IPR009057 - Homeodomain-like
IPR001005 - SANT/Myb
IPR017884 - SANT_dom

Pfam Domains:
PF01448 - ELM2 domain
PF19426 - Mesoderm induction early response protein 1 beta C-terminal

SCOP Domains:
46689 - Homeodomain-like

ModBase Predicted Comparative 3D Structure on Q8N108
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003677 DNA binding
GO:0004407 histone deacetylase activity
GO:0005515 protein binding
GO:0042826 histone deacetylase binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0016575 histone deacetylation
GO:0031937 positive regulation of chromatin silencing
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0017053 transcriptional repressor complex
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  AK302061 - Homo sapiens cDNA FLJ60095 complete cds, highly similar to Mesoderm induction early response protein 1.
AL831987 - Homo sapiens mRNA; cDNA DKFZp451G243 (from clone DKFZp451G243).
LP895147 - Sequence 11 from Patent EP3253886.
AY124189 - Homo sapiens mesoderm induction early response 1 N1-beta-i (MIER1) mRNA, complete cds; alternatively spliced.
AY124190 - Homo sapiens mesoderm induction early response 1 N2-beta-i (MIER1) mRNA, complete cds; alternatively spliced.
AY124186 - Homo sapiens mesoderm induction early response 1 N1-alpha (MIER1) mRNA, complete cds; alternatively spliced.
AY124187 - Homo sapiens mesoderm induction early response 1 N2-alpha (MIER1) mRNA, complete cds; alternatively spliced.
AY124192 - Homo sapiens mesoderm induction early response 1 N1-beta-ii (MIER1) mRNA, complete cds; alternatively spliced.
AY124193 - Homo sapiens mesoderm induction early response 1 N2-beta-ii (MIER1) mRNA, complete cds; alternatively spliced.
AF515447 - Homo sapiens mesoderm induction early response 1 N2-beta mRNA, complete cds, alternatively spliced.
AF515448 - Homo sapiens mesoderm induction early response 1 N1-beta mRNA, complete cds, alternatively spliced.
BC066898 - Homo sapiens mesoderm induction early response 1 homolog (Xenopus laevis), mRNA (cDNA clone IMAGE:4822246), complete cds.
AK307717 - Homo sapiens cDNA, FLJ97665.
JD419182 - Sequence 400206 from Patent EP1572962.
BC108726 - Homo sapiens mesoderm induction early response 1 homolog (Xenopus laevis), mRNA (cDNA clone MGC:131940 IMAGE:5194296), complete cds.
CR627441 - Homo sapiens mRNA; cDNA DKFZp781G0451 (from clone DKFZp781G0451).
AB590466 - Synthetic construct DNA, clone: pFN21AE1157, Homo sapiens MIER1 gene for mesoderm induction early response 1 homolog, without stop codon, in Flexi system.
BC125217 - Homo sapiens mesoderm induction early response 1 homolog (Xenopus laevis), mRNA (cDNA clone MGC:150640 IMAGE:40123797), complete cds.
BC125218 - Homo sapiens mesoderm induction early response 1 homolog (Xenopus laevis), mRNA (cDNA clone MGC:150641 IMAGE:40123803), complete cds.
JD464900 - Sequence 445924 from Patent EP1572962.
AY124191 - Homo sapiens mesoderm induction early response 1 N3-beta-i (MIER1) mRNA, complete cds; alternatively spliced.
AY124188 - Homo sapiens mesoderm induction early response 1 N3-alpha (MIER1) mRNA, complete cds; alternatively spliced.
AY124194 - Homo sapiens mesoderm induction early response 1 N3-beta-ii (MIER1) mRNA, complete cds; alternatively spliced.
AF515446 - Homo sapiens mesoderm induction early response 1 N3-beta mRNA, complete cds, alternatively spliced.
BC017423 - Homo sapiens mesoderm induction early response 1 homolog (Xenopus laevis), mRNA (cDNA clone IMAGE:4692746), complete cds.
AB046830 - Homo sapiens mRNA for KIAA1610 protein, partial cds.
KJ906282 - Synthetic construct Homo sapiens clone ccsbBroadEn_15952 MIER1 gene, encodes complete protein.
KJ894451 - Synthetic construct Homo sapiens clone ccsbBroadEn_03845 MIER1 gene, encodes complete protein.
JD168301 - Sequence 149325 from Patent EP1572962.
JD303181 - Sequence 284205 from Patent EP1572962.
JD313428 - Sequence 294452 from Patent EP1572962.
JD524416 - Sequence 505440 from Patent EP1572962.
JD363333 - Sequence 344357 from Patent EP1572962.
JD491506 - Sequence 472530 from Patent EP1572962.
JD109915 - Sequence 90939 from Patent EP1572962.
JD429404 - Sequence 410428 from Patent EP1572962.
JD275185 - Sequence 256209 from Patent EP1572962.
JD495129 - Sequence 476153 from Patent EP1572962.
JD328924 - Sequence 309948 from Patent EP1572962.
JD163413 - Sequence 144437 from Patent EP1572962.
JD490300 - Sequence 471324 from Patent EP1572962.
JD185021 - Sequence 166045 from Patent EP1572962.
JD039819 - Sequence 20843 from Patent EP1572962.
JD312921 - Sequence 293945 from Patent EP1572962.
JD516771 - Sequence 497795 from Patent EP1572962.
JD091189 - Sequence 72213 from Patent EP1572962.
JD048500 - Sequence 29524 from Patent EP1572962.
JD109384 - Sequence 90408 from Patent EP1572962.
JD091050 - Sequence 72074 from Patent EP1572962.
JD196198 - Sequence 177222 from Patent EP1572962.
JD525477 - Sequence 506501 from Patent EP1572962.
JD101320 - Sequence 82344 from Patent EP1572962.
JD358016 - Sequence 339040 from Patent EP1572962.
JD221706 - Sequence 202730 from Patent EP1572962.
JD565010 - Sequence 546034 from Patent EP1572962.
JD126465 - Sequence 107489 from Patent EP1572962.
JD251701 - Sequence 232725 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: C9JFD4, ENST00000401041.1, ENST00000401041.2, ENST00000401041.3, ENST00000401041.4, ENST00000401041.5, KIAA1610, MIER1_HUMAN, NM_001350530, Q08AE0, Q32NC4, Q5T104, Q5TAD1, Q5TAD2, Q5TAD4, Q5TAD5, Q6AHY8, Q86TB4, Q8N108, Q8N156, Q8N161, Q8NC37, Q8NES4, Q8NES5, Q8NES6, Q8WWG2, Q9HCG2, uc319bdi.1, uc319bdi.2
UCSC ID: ENST00000401041.6_6
RefSeq Accession: NM_001077700.3
Protein: Q8N108 (aka MIER1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.