Human Gene MRTFB (ENST00000318282.9_4) from GENCODE V47lift37
  Description: myocardin related transcription factor B, transcript variant 2 (from RefSeq NM_014048.4)
Gencode Transcript: ENST00000318282.9_4
Gencode Gene: ENSG00000186260.17_14
Transcript (Including UTRs)
   Position: hg19 chr16:14,173,145-14,360,629 Size: 187,485 Total Exon Count: 16 Strand: +
Coding Region
   Position: hg19 chr16:14,234,464-14,355,301 Size: 120,838 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:14,173,145-14,360,629)mRNA (may differ from genome)Protein (1049 aa)
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-  Comments and Description Text from UniProtKB
  ID: MKL2_HUMAN
DESCRIPTION: RecName: Full=MKL/myocardin-like protein 2; AltName: Full=Megakaryoblastic leukemia 2; AltName: Full=Myocardin-related transcription factor B; Short=MRTF-B;
FUNCTION: Acts as a transcriptional coactivator of serum response factor (SRF). Required for skeletal myogenic differentiation.
SUBUNIT: Interacts with MKL1 and SRF.
INTERACTION: P11831:SRF; NbExp=3; IntAct=EBI-493007, EBI-493034;
SUBCELLULAR LOCATION: Nucleus (By similarity).
DOMAIN: The N-terminal region is required for nuclear localization and the C-terminal region mediates transcriptional activity (By similarity).
PTM: O-glycosylated (By similarity).
SIMILARITY: Contains 3 RPEL repeats.
SIMILARITY: Contains 1 SAP domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 14.68 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 352.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -24.70130-0.190 Picture PostScript Text
3' UTR -1442.505328-0.271 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004018 - RPEL_repeat
IPR003034 - SAP_DNA-bd

Pfam Domains:
PF02037 - SAP domain
PF02755 - RPEL repeat

SCOP Domains:
68906 - SAP domain

ModBase Predicted Comparative 3D Structure on Q9ULH7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0001105 RNA polymerase II transcription coactivator activity
GO:0003713 transcription coactivator activity
GO:0005515 protein binding
GO:0045296 cadherin binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006366 transcription from RNA polymerase II promoter
GO:0007275 multicellular organism development
GO:0007517 muscle organ development
GO:0030154 cell differentiation
GO:0045844 positive regulation of striated muscle tissue development
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0051145 smooth muscle cell differentiation

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AK294765 - Homo sapiens cDNA FLJ61243 complete cds, highly similar to MKL/myocardin-like protein 2.
AY374297 - Homo sapiens megakaryoblastic leukemia 2 (MKL2) mRNA, complete cds.
BC034934 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:4907072), partial cds.
BC171750 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone MGC:198464 IMAGE:9053251), complete cds.
BC136260 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone MGC:167870 IMAGE:9020247), complete cds.
BC127941 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:40132674), partial cds.
AK093577 - Homo sapiens cDNA FLJ36258 fis, clone THYMU2002450.
AX748211 - Sequence 1736 from Patent EP1308459.
AK126948 - Homo sapiens cDNA FLJ45001 fis, clone BRAWH3011402.
BC047761 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:5741919), complete cds.
CU690492 - Synthetic construct Homo sapiens gateway clone IMAGE:100022749 5' read MKL2 mRNA.
KJ902959 - Synthetic construct Homo sapiens clone ccsbBroadEn_12353 MKL2 gene, encodes complete protein.
JD547399 - Sequence 528423 from Patent EP1572962.
AB033069 - Homo sapiens mRNA for KIAA1243 protein, partial cds.
CR749797 - Homo sapiens mRNA; cDNA DKFZp686J1745 (from clone DKFZp686J1745).
AK127530 - Homo sapiens cDNA FLJ45623 fis, clone BRTHA3027957, highly similar to MKL/myocardin-like protein 2.
BC073950 - Homo sapiens cDNA clone IMAGE:5521559, containing frame-shift errors.
BX641041 - Homo sapiens mRNA; cDNA DKFZp686P16110 (from clone DKFZp686P16110).
JD046615 - Sequence 27639 from Patent EP1572962.
JD119445 - Sequence 100469 from Patent EP1572962.
JD442671 - Sequence 423695 from Patent EP1572962.
JD334803 - Sequence 315827 from Patent EP1572962.
JD098190 - Sequence 79214 from Patent EP1572962.
JD217012 - Sequence 198036 from Patent EP1572962.
JD217212 - Sequence 198236 from Patent EP1572962.
AK056385 - Homo sapiens cDNA FLJ31823 fis, clone NT2RP6000059.
JD022267 - Sequence 3291 from Patent EP1572962.
JD030585 - Sequence 11609 from Patent EP1572962.
JD028113 - Sequence 9137 from Patent EP1572962.
BC067270 - Homo sapiens cDNA clone IMAGE:6174056, partial cds.
AK057216 - Homo sapiens cDNA FLJ32654 fis, clone TESTI1000023.
BC107868 - Homo sapiens cDNA clone IMAGE:4732957.
BC016293 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:3911407).
AF078853 - Homo sapiens NPD001 mRNA, complete cds.
JD090629 - Sequence 71653 from Patent EP1572962.
JD305834 - Sequence 286858 from Patent EP1572962.
BC030510 - Homo sapiens cDNA clone IMAGE:4526353.
BC038834 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:4391226), with apparent retained intron.
JD305807 - Sequence 286831 from Patent EP1572962.
BC006977 - Homo sapiens MKL/myocardin-like 2, mRNA (cDNA clone IMAGE:3828127).
JD353622 - Sequence 334646 from Patent EP1572962.
BC020644 - Homo sapiens cDNA clone IMAGE:4809499.
BC050541 - Homo sapiens cDNA clone IMAGE:4157409.
JD156611 - Sequence 137635 from Patent EP1572962.
JD359054 - Sequence 340078 from Patent EP1572962.
JD176003 - Sequence 157027 from Patent EP1572962.
JD359913 - Sequence 340937 from Patent EP1572962.
JD089869 - Sequence 70893 from Patent EP1572962.
JD528561 - Sequence 509585 from Patent EP1572962.
JD314727 - Sequence 295751 from Patent EP1572962.
JD300453 - Sequence 281477 from Patent EP1572962.
JD038879 - Sequence 19903 from Patent EP1572962.
JD451837 - Sequence 432861 from Patent EP1572962.
JD084969 - Sequence 65993 from Patent EP1572962.
JD203121 - Sequence 184145 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A6ND53, B4DGT8, ENST00000318282.1, ENST00000318282.2, ENST00000318282.3, ENST00000318282.4, ENST00000318282.5, ENST00000318282.6, ENST00000318282.7, ENST00000318282.8, KIAA1243, MKL2 , MRTFB , MRTFB_HUMAN, NM_014048, Q68CT1, Q6UB16, Q86WW2, Q8N226, Q9ULH7, uc317qlg.1
UCSC ID: ENST00000318282.9_4
RefSeq Accession: NM_014048.4
Protein: Q9ULH7 (aka MKL2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.