Human Gene MMP14 (ENST00000311852.11_4) from GENCODE V47lift37
  Description: matrix metallopeptidase 14 (from RefSeq NM_004995.4)
Gencode Transcript: ENST00000311852.11_4
Gencode Gene: ENSG00000157227.14_8
Transcript (Including UTRs)
   Position: hg19 chr14:23,305,794-23,316,967 Size: 11,174 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr14:23,306,027-23,315,248 Size: 9,222 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr14:23,305,794-23,316,967)mRNA (may differ from genome)Protein (582 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MMP14_HUMAN
DESCRIPTION: RecName: Full=Matrix metalloproteinase-14; Short=MMP-14; EC=3.4.24.80; AltName: Full=MMP-X1; AltName: Full=Membrane-type matrix metalloproteinase 1; Short=MT-MMP 1; Short=MTMMP1; AltName: Full=Membrane-type-1 matrix metalloproteinase; Short=MT1-MMP; Short=MT1MMP; Flags: Precursor;
FUNCTION: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15.
CATALYTIC ACTIVITY: Endopeptidase activity. Activates progelatinase A by cleavage of the propeptide at 37-Asn-|-Leu-38. Other bonds hydrolyzed include 35-Gly-|-Ile-36 in the propeptide of collagenase 3, and 341-Asn-|-Phe-342, 441-Asp-|-Leu-442 and 354-Gln-|-Thr-355 in the aggrecan interglobular domain.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
COFACTOR: Calcium (By similarity).
SUBUNIT: Interacts (via C-terminal cytoplasmic tail) with BST2.
INTERACTION: Q9BV57:ADI1; NbExp=4; IntAct=EBI-992788, EBI-992807; P53667:LIMK1; NbExp=4; IntAct=EBI-992788, EBI-444403;
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm.
TISSUE SPECIFICITY: Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
INDUCTION: Up-regulated by NANOS1.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Belongs to the peptidase M10A family.
SIMILARITY: Contains 4 hemopexin-like domains.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mmp14/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MMP14
Diseases sorted by gene-association score: winchester syndrome* (1288), multicentric osteolysis, nodulosis, and arthropathy* (530), fibrosarcoma (11), light chain deposition disease (10), klatskin's tumor (9), actinic keratosis (7), serous cystadenocarcinoma (6), lymph node disease (6), nodular malignant melanoma (6), lymphangioleiomyomatosis (6), parotid gland cancer (5), malignant glioma (3), ovarian cancer, somatic (3), colorectal cancer (2), breast cancer (2), osteoporosis (2), squamous cell carcinoma, head and neck (2), prostate cancer (2)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 223.92 RPKM in Cells - Cultured fibroblasts
Total median expression: 2291.42 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -77.70233-0.333 Picture PostScript Text
3' UTR -772.501719-0.449 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000585 - Hemopexin/matrixin
IPR018486 - Hemopexin/matrixin_CS
IPR018487 - Hemopexin/matrixin_repeat
IPR024079 - MetalloPept_cat_dom
IPR001818 - Pept_M10_metallopeptidase
IPR016293 - Pept_M10A_matrix_strom
IPR021190 - Pept_M10A_matrixin
IPR021805 - Pept_M10A_metallopeptidase_C
IPR021158 - Pept_M10A_Zn_BS
IPR006026 - Peptidase_Metallo
IPR002477 - Peptidoglycan-bd-like

Pfam Domains:
PF00045 - Hemopexin
PF00413 - Matrixin
PF01471 - Putative peptidoglycan binding domain
PF11857 - Domain of unknown function (DUF3377)

SCOP Domains:
47090 - PGBD-like
50923 - Hemopexin-like domain
50993 - Peptidase/esterase 'gauge' domain
55486 - Metalloproteases ("zincins"), catalytic domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1BQQ - X-ray MuPIT 1BUV - X-ray 3C7X - X-ray MuPIT 3MA2 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P50281
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004222 metalloendopeptidase activity
GO:0004252 serine-type endopeptidase activity
GO:0005178 integrin binding
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0016504 peptidase activator activity
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity

Biological Process:
GO:0001503 ossification
GO:0001525 angiogenesis
GO:0001541 ovarian follicle development
GO:0001666 response to hypoxia
GO:0001935 endothelial cell proliferation
GO:0001958 endochondral ossification
GO:0006508 proteolysis
GO:0006979 response to oxidative stress
GO:0008584 male gonad development
GO:0009612 response to mechanical stimulus
GO:0009725 response to hormone
GO:0010831 positive regulation of myotube differentiation
GO:0010952 positive regulation of peptidase activity
GO:0014070 response to organic cyclic compound
GO:0016477 cell migration
GO:0016485 protein processing
GO:0022617 extracellular matrix disassembly
GO:0030307 positive regulation of cell growth
GO:0030324 lung development
GO:0030335 positive regulation of cell migration
GO:0030574 collagen catabolic process
GO:0031638 zymogen activation
GO:0035987 endodermal cell differentiation
GO:0035988 chondrocyte proliferation
GO:0043615 astrocyte cell migration
GO:0043627 response to estrogen
GO:0045579 positive regulation of B cell differentiation
GO:0045746 negative regulation of Notch signaling pathway
GO:0048701 embryonic cranial skeleton morphogenesis
GO:0048754 branching morphogenesis of an epithelial tube
GO:0048771 tissue remodeling
GO:0048870 cell motility
GO:0051895 negative regulation of focal adhesion assembly
GO:0060322 head development
GO:0060348 bone development
GO:0097094 craniofacial suture morphogenesis
GO:1905523 positive regulation of macrophage migration
GO:1990834 response to odorant

Cellular Component:
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005796 Golgi lumen
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0005925 focal adhesion
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031012 extracellular matrix
GO:0031410 cytoplasmic vesicle
GO:0042470 melanosome
GO:0044354 macropinosome
GO:0045111 intermediate filament cytoskeleton


-  Descriptions from all associated GenBank mRNAs
  LF210068 - JP 2014500723-A/17571: Polycomb-Associated Non-Coding RNAs.
HE978355 - Homo sapiens mRNA for matrix metalloproteinase 14 (MMP14), Coriell cell line GM0229.
AK312657 - Homo sapiens cDNA, FLJ93047, highly similar to Homo sapiens matrix metallopeptidase 14 (membrane-inserted) (MMP14), mRNA.
JD439563 - Sequence 420587 from Patent EP1572962.
AK291325 - Homo sapiens cDNA FLJ78066 complete cds, highly similar to Homo sapiens matrix metallopeptidase 14 (membrane-inserted), mRNA.
BC064803 - Homo sapiens matrix metallopeptidase 14 (membrane-inserted), mRNA (cDNA clone MGC:64960 IMAGE:6046773), complete cds.
JD394541 - Sequence 375565 from Patent EP1572962.
JD202580 - Sequence 183604 from Patent EP1572962.
BC039581 - Homo sapiens matrix metallopeptidase 14 (membrane-inserted), mRNA (cDNA clone IMAGE:6048471), containing frame-shift errors.
JD065545 - Sequence 46569 from Patent EP1572962.
X83535 - Homo sapiens mRNA for membrane-type matrix metalloproteinase.
Z48481 - H.sapiens mRNA for membrane-type matrix metalloproteinase 1.
X90925 - H.sapiens mRNA for MT-MMP protein.
D26512 - Homo sapiens MT-MMP mRNA for membrane-type matrix metalloproteinase, comlete cds.
E09720 - cDNA encoding novel human matrix metalloprotease.
E10297 - cDNA encoding novel human matrix metalloprotease.
JD526189 - Sequence 507213 from Patent EP1572962.
AB385035 - Synthetic construct DNA, clone: pF1KB5096, Homo sapiens MMP14 gene for matrix metalloproteinase-14 precursor, complete cds, without stop codon, in Flexi system.
CU687944 - Synthetic construct Homo sapiens gateway clone IMAGE:100021796 5' read MMP14 mRNA.
KJ897198 - Synthetic construct Homo sapiens clone ccsbBroadEn_06592 MMP14 gene, encodes complete protein.
U41078 - Human membrane-type matrix metalloproteinase-1 mRNA, complete cds.
LF334425 - JP 2014500723-A/141928: Polycomb-Associated Non-Coding RNAs.
LF334427 - JP 2014500723-A/141930: Polycomb-Associated Non-Coding RNAs.
LF334428 - JP 2014500723-A/141931: Polycomb-Associated Non-Coding RNAs.
LF334430 - JP 2014500723-A/141933: Polycomb-Associated Non-Coding RNAs.
LF334432 - JP 2014500723-A/141935: Polycomb-Associated Non-Coding RNAs.
LF334435 - JP 2014500723-A/141938: Polycomb-Associated Non-Coding RNAs.
LF334436 - JP 2014500723-A/141939: Polycomb-Associated Non-Coding RNAs.
LF334437 - JP 2014500723-A/141940: Polycomb-Associated Non-Coding RNAs.
LF334440 - JP 2014500723-A/141943: Polycomb-Associated Non-Coding RNAs.
LF334441 - JP 2014500723-A/141944: Polycomb-Associated Non-Coding RNAs.
LF334442 - JP 2014500723-A/141945: Polycomb-Associated Non-Coding RNAs.
LF334444 - JP 2014500723-A/141947: Polycomb-Associated Non-Coding RNAs.
JD337048 - Sequence 318072 from Patent EP1572962.
LF334446 - JP 2014500723-A/141949: Polycomb-Associated Non-Coding RNAs.
JD114645 - Sequence 95669 from Patent EP1572962.
JD155044 - Sequence 136068 from Patent EP1572962.
JD226585 - Sequence 207609 from Patent EP1572962.
JD389788 - Sequence 370812 from Patent EP1572962.
JD498327 - Sequence 479351 from Patent EP1572962.
JD127239 - Sequence 108263 from Patent EP1572962.
LF334447 - JP 2014500723-A/141950: Polycomb-Associated Non-Coding RNAs.
JD386250 - Sequence 367274 from Patent EP1572962.
JD329549 - Sequence 310573 from Patent EP1572962.
JD240218 - Sequence 221242 from Patent EP1572962.
JD323356 - Sequence 304380 from Patent EP1572962.
JD284437 - Sequence 265461 from Patent EP1572962.
JD322225 - Sequence 303249 from Patent EP1572962.
LF334449 - JP 2014500723-A/141952: Polycomb-Associated Non-Coding RNAs.
JD218942 - Sequence 199966 from Patent EP1572962.
LF334450 - JP 2014500723-A/141953: Polycomb-Associated Non-Coding RNAs.
JD433845 - Sequence 414869 from Patent EP1572962.
JD362832 - Sequence 343856 from Patent EP1572962.
JD056013 - Sequence 37037 from Patent EP1572962.
JD444347 - Sequence 425371 from Patent EP1572962.
LF334452 - JP 2014500723-A/141955: Polycomb-Associated Non-Coding RNAs.
JD492727 - Sequence 473751 from Patent EP1572962.
JD544425 - Sequence 525449 from Patent EP1572962.
JD431592 - Sequence 412616 from Patent EP1572962.
LF334453 - JP 2014500723-A/141956: Polycomb-Associated Non-Coding RNAs.
JD535293 - Sequence 516317 from Patent EP1572962.
JD469113 - Sequence 450137 from Patent EP1572962.
JD271775 - Sequence 252799 from Patent EP1572962.
LF334454 - JP 2014500723-A/141957: Polycomb-Associated Non-Coding RNAs.
JD457261 - Sequence 438285 from Patent EP1572962.
JD320298 - Sequence 301322 from Patent EP1572962.
LF334456 - JP 2014500723-A/141959: Polycomb-Associated Non-Coding RNAs.
JD165464 - Sequence 146488 from Patent EP1572962.
JD165394 - Sequence 146418 from Patent EP1572962.
JD561242 - Sequence 542266 from Patent EP1572962.
JD385256 - Sequence 366280 from Patent EP1572962.
LF334457 - JP 2014500723-A/141960: Polycomb-Associated Non-Coding RNAs.
LF334458 - JP 2014500723-A/141961: Polycomb-Associated Non-Coding RNAs.
MA445645 - JP 2018138019-A/17571: Polycomb-Associated Non-Coding RNAs.
MA570002 - JP 2018138019-A/141928: Polycomb-Associated Non-Coding RNAs.
MA570004 - JP 2018138019-A/141930: Polycomb-Associated Non-Coding RNAs.
MA570005 - JP 2018138019-A/141931: Polycomb-Associated Non-Coding RNAs.
MA570007 - JP 2018138019-A/141933: Polycomb-Associated Non-Coding RNAs.
MA570009 - JP 2018138019-A/141935: Polycomb-Associated Non-Coding RNAs.
MA570012 - JP 2018138019-A/141938: Polycomb-Associated Non-Coding RNAs.
MA570013 - JP 2018138019-A/141939: Polycomb-Associated Non-Coding RNAs.
MA570014 - JP 2018138019-A/141940: Polycomb-Associated Non-Coding RNAs.
MA570017 - JP 2018138019-A/141943: Polycomb-Associated Non-Coding RNAs.
MA570018 - JP 2018138019-A/141944: Polycomb-Associated Non-Coding RNAs.
MA570019 - JP 2018138019-A/141945: Polycomb-Associated Non-Coding RNAs.
MA570021 - JP 2018138019-A/141947: Polycomb-Associated Non-Coding RNAs.
MA570023 - JP 2018138019-A/141949: Polycomb-Associated Non-Coding RNAs.
MA570024 - JP 2018138019-A/141950: Polycomb-Associated Non-Coding RNAs.
MA570026 - JP 2018138019-A/141952: Polycomb-Associated Non-Coding RNAs.
MA570027 - JP 2018138019-A/141953: Polycomb-Associated Non-Coding RNAs.
MA570029 - JP 2018138019-A/141955: Polycomb-Associated Non-Coding RNAs.
MA570030 - JP 2018138019-A/141956: Polycomb-Associated Non-Coding RNAs.
MA570031 - JP 2018138019-A/141957: Polycomb-Associated Non-Coding RNAs.
MA570033 - JP 2018138019-A/141959: Polycomb-Associated Non-Coding RNAs.
MA570034 - JP 2018138019-A/141960: Polycomb-Associated Non-Coding RNAs.
MA570035 - JP 2018138019-A/141961: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_reckPathway - Inhibition of Matrix Metalloproteinases

Reactome (by CSHL, EBI, and GO)

Protein P50281 (Reactome details) participates in the following event(s):

R-HSA-1592349 MMP14 (MT1-MMP) binds TIMP2
R-HSA-1604350 MMP14:TIMP2 binds proMMP2
R-HSA-1602466 Activation of MT-MMPs by FURIN
R-HSA-1604368 Autocatalytic activation of bound proMMP2
R-HSA-1604360 Initial activation of proMMP2 by MMP14
R-NUL-2533964 Nid1 degradation by MMP14, MMP15
R-HSA-2533965 NID1 degradation by MMP14, MMP15
R-HSA-2534240 HSPG2 (perlecan) degradation by MMP14, MMP15
R-HSA-1604741 Initial activation of proMMP13 by MMP14 (MT1-MMP)
R-HSA-1458433 Collagen type I degradation by MMP14
R-HSA-1474196 Collagen type II degradation by MMP14
R-HSA-1474210 Collagen type III degradation by MMP14
R-NUL-2473583 Collagen type I degradation by MMP14
R-NUL-2473590 Collagen type II degradation by MMP14
R-HSA-2514831 Fibrillin-1 degradation by MMP14
R-HSA-2533874 Laminin-511 degradation by MMP14
R-NUL-2533890 Laminin-511 degradation by MMP14
R-HSA-2533950 Fibronectin degradation by MMP14, TMPRSS6
R-HSA-3791155 Laminin-322 degradation by MMP14
R-HSA-3828025 DCN (decorin) degradation by MMP14
R-HSA-1592389 Activation of Matrix Metalloproteinases
R-HSA-1474228 Degradation of the extracellular matrix
R-HSA-1442490 Collagen degradation
R-HSA-1474244 Extracellular matrix organization

-  Other Names for This Gene
  Alternate Gene Symbols: A8K5L0, ENST00000311852.1, ENST00000311852.10, ENST00000311852.2, ENST00000311852.3, ENST00000311852.4, ENST00000311852.5, ENST00000311852.6, ENST00000311852.7, ENST00000311852.8, ENST00000311852.9, MMP14_HUMAN, NM_004995, P50281, Q6GSF3, Q92678, uc317pbz.1, uc317pbz.2
UCSC ID: ENST00000311852.11_4
RefSeq Accession: NM_004995.4
Protein: P50281 (aka MMP14_HUMAN or MM14_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.