Human Gene MMP15 (ENST00000219271.4_4) from GENCODE V47lift37
  Description: matrix metallopeptidase 15 (from RefSeq NM_002428.4)
Gencode Transcript: ENST00000219271.4_4
Gencode Gene: ENSG00000102996.5_8
Transcript (Including UTRs)
   Position: hg19 chr16:58,059,658-58,080,805 Size: 21,148 Total Exon Count: 10 Strand: +
Coding Region
   Position: hg19 chr16:58,060,255-58,079,350 Size: 19,096 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:58,059,658-58,080,805)mRNA (may differ from genome)Protein (669 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MMP15_HUMAN
DESCRIPTION: RecName: Full=Matrix metalloproteinase-15; Short=MMP-15; EC=3.4.24.-; AltName: Full=Membrane-type matrix metalloproteinase 2; Short=MT-MMP 2; Short=MTMMP2; AltName: Full=Membrane-type-2 matrix metalloproteinase; Short=MT2-MMP; Short=MT2MMP; AltName: Full=SMCP-2; Flags: Precursor;
FUNCTION: Endopeptidase that degrades various components of the extracellular matrix. May activate progelatinase A.
COFACTOR: Binds 1 zinc ion per subunit (By similarity).
COFACTOR: Calcium (By similarity).
INTERACTION: P04070:PROC; NbExp=2; IntAct=EBI-1383043, EBI-1383018;
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein; Extracellular side (Potential).
TISSUE SPECIFICITY: Appeared to be synthesized preferentially in liver, placenta, testis, colon and intestine. Substantial amounts are also detected in pancreas, kidney, lung, heart and skeletal muscle.
DOMAIN: The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
PTM: The precursor is cleaved by a furin endopeptidase (By similarity).
SIMILARITY: Belongs to the peptidase M10A family.
SIMILARITY: Contains 4 hemopexin-like domains.
SEQUENCE CAUTION: Sequence=BAA13071.1; Type=Erroneous initiation;
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/mmp15/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 35.99 RPKM in Thyroid
Total median expression: 358.75 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -355.70597-0.596 Picture PostScript Text
3' UTR -640.801455-0.440 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000585 - Hemopexin/matrixin
IPR018486 - Hemopexin/matrixin_CS
IPR018487 - Hemopexin/matrixin_repeat
IPR024079 - MetalloPept_cat_dom
IPR001818 - Pept_M10_metallopeptidase
IPR016293 - Pept_M10A_matrix_strom
IPR021190 - Pept_M10A_matrixin
IPR021805 - Pept_M10A_metallopeptidase_C
IPR021158 - Pept_M10A_Zn_BS
IPR006026 - Peptidase_Metallo
IPR002477 - Peptidoglycan-bd-like

Pfam Domains:
PF00045 - Hemopexin
PF00413 - Matrixin
PF01471 - Putative peptidoglycan binding domain
PF11857 - Domain of unknown function (DUF3377)

SCOP Domains:
47090 - PGBD-like
50923 - Hemopexin-like domain
55486 - Metalloproteases ("zincins"), catalytic domain

ModBase Predicted Comparative 3D Structure on P51511
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004222 metalloendopeptidase activity
GO:0005515 protein binding
GO:0008047 enzyme activator activity
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity

Biological Process:
GO:0006464 cellular protein modification process
GO:0006508 proteolysis
GO:0022617 extracellular matrix disassembly
GO:0030574 collagen catabolic process
GO:0032355 response to estradiol
GO:0035987 endodermal cell differentiation
GO:0043085 positive regulation of catalytic activity

Cellular Component:
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0031012 extracellular matrix


-  Descriptions from all associated GenBank mRNAs
  BC036495 - Homo sapiens matrix metallopeptidase 15 (membrane-inserted), mRNA (cDNA clone MGC:43196 IMAGE:5263145), complete cds.
BC055428 - Homo sapiens matrix metallopeptidase 15 (membrane-inserted), mRNA (cDNA clone MGC:51077 IMAGE:4811572), complete cds.
Z48482 - H.sapiens mRNA for membrane-type matrix metalloproteinase 2.
AB527713 - Synthetic construct DNA, clone: pF1KB6215, Homo sapiens MMP15 gene for matrix metallopeptidase 15, without stop codon, in Flexi system.
BC006412 - Homo sapiens matrix metallopeptidase 15 (membrane-inserted), mRNA (cDNA clone IMAGE:3607064).
D86331 - Homo sapiens MT2-MMP gene for matrix metalloprotein, complete cds.
BT006715 - Homo sapiens matrix metalloproteinase 15 (membrane-inserted) mRNA, complete cds.
KJ901579 - Synthetic construct Homo sapiens clone ccsbBroadEn_10973 MMP15 gene, encodes complete protein.
D85510 - Homo sapiens mRNA for SMCP-2, partial cds.
DQ600662 - Homo sapiens piRNA piR-38728, complete sequence.
JD357024 - Sequence 338048 from Patent EP1572962.
JD552126 - Sequence 533150 from Patent EP1572962.
JD385839 - Sequence 366863 from Patent EP1572962.
JD560513 - Sequence 541537 from Patent EP1572962.
JD068135 - Sequence 49159 from Patent EP1572962.
JD053246 - Sequence 34270 from Patent EP1572962.
JD193553 - Sequence 174577 from Patent EP1572962.
JD122023 - Sequence 103047 from Patent EP1572962.
JD384267 - Sequence 365291 from Patent EP1572962.
JD521484 - Sequence 502508 from Patent EP1572962.
JD552031 - Sequence 533055 from Patent EP1572962.
JD187130 - Sequence 168154 from Patent EP1572962.
JD388576 - Sequence 369600 from Patent EP1572962.
JD107006 - Sequence 88030 from Patent EP1572962.
JD045588 - Sequence 26612 from Patent EP1572962.
JD540064 - Sequence 521088 from Patent EP1572962.
JD125249 - Sequence 106273 from Patent EP1572962.
JD187140 - Sequence 168164 from Patent EP1572962.
JD387776 - Sequence 368800 from Patent EP1572962.
JD540099 - Sequence 521123 from Patent EP1572962.
JD343822 - Sequence 324846 from Patent EP1572962.
JD468682 - Sequence 449706 from Patent EP1572962.
JD169855 - Sequence 150879 from Patent EP1572962.
JD555134 - Sequence 536158 from Patent EP1572962.
JD131857 - Sequence 112881 from Patent EP1572962.
JD149546 - Sequence 130570 from Patent EP1572962.
JD389051 - Sequence 370075 from Patent EP1572962.
JD223266 - Sequence 204290 from Patent EP1572962.
JD060675 - Sequence 41699 from Patent EP1572962.
JD117616 - Sequence 98640 from Patent EP1572962.
JD227267 - Sequence 208291 from Patent EP1572962.
JD204887 - Sequence 185911 from Patent EP1572962.
JD335596 - Sequence 316620 from Patent EP1572962.
JD470010 - Sequence 451034 from Patent EP1572962.
JD536805 - Sequence 517829 from Patent EP1572962.
JD545471 - Sequence 526495 from Patent EP1572962.
JD280257 - Sequence 261281 from Patent EP1572962.
JD317766 - Sequence 298790 from Patent EP1572962.
JD119637 - Sequence 100661 from Patent EP1572962.
JD133090 - Sequence 114114 from Patent EP1572962.
JD423086 - Sequence 404110 from Patent EP1572962.
JD077691 - Sequence 58715 from Patent EP1572962.
JD313272 - Sequence 294296 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein P51511 (Reactome details) participates in the following event(s):

R-HSA-1602466 Activation of MT-MMPs by FURIN
R-HSA-2473596 Collagen type I degradation by MMP15
R-NUL-2533964 Nid1 degradation by MMP14, MMP15
R-HSA-2533965 NID1 degradation by MMP14, MMP15
R-HSA-2534240 HSPG2 (perlecan) degradation by MMP14, MMP15
R-HSA-1592389 Activation of Matrix Metalloproteinases
R-HSA-1442490 Collagen degradation
R-HSA-1474228 Degradation of the extracellular matrix
R-HSA-1474244 Extracellular matrix organization

-  Other Names for This Gene
  Alternate Gene Symbols: A0A2U6, ENST00000219271.1, ENST00000219271.2, ENST00000219271.3, MMP15_HUMAN, NM_002428, P51511, Q14111, uc317cww.1, uc317cww.2
UCSC ID: ENST00000219271.4_4
RefSeq Accession: NM_002428.4
Protein: P51511 (aka MMP15_HUMAN or MM15_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.