Human Gene MRPL22 (ENST00000523037.6_10) from GENCODE V47lift37
  Description: mitochondrial ribosomal protein L22, transcript variant 1 (from RefSeq NM_014180.4)
Gencode Transcript: ENST00000523037.6_10
Gencode Gene: ENSG00000082515.18_13
Transcript (Including UTRs)
   Position: hg19 chr5:154,320,633-154,348,971 Size: 28,339 Total Exon Count: 7 Strand: +
Coding Region
   Position: hg19 chr5:154,320,671-154,346,457 Size: 25,787 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:154,320,633-154,348,971)mRNA (may differ from genome)Protein (206 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RM22_HUMAN
DESCRIPTION: RecName: Full=39S ribosomal protein L22, mitochondrial; Short=L22mt; Short=MRP-L22; AltName: Full=39S ribosomal protein L25, mitochondrial; Short=L25mt; Short=MRP-L25; Flags: Precursor;
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: Belongs to the ribosomal protein L22P family.
SEQUENCE CAUTION: Sequence=AAF29122.1; Type=Frameshift; Positions=197;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.21 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 236.87 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -11.4038-0.300 Picture PostScript Text
3' UTR -676.302514-0.269 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001063 - Ribosomal_L22
IPR005727 - Ribosomal_L22_bac/chlpt-type

Pfam Domains:
PF00237 - Ribosomal protein L22p/L17e

SCOP Domains:
54843 - Ribosomal protein L22

ModBase Predicted Comparative 3D Structure on Q9NWU5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003735 structural constituent of ribosome

Biological Process:
GO:0006412 translation
GO:0070125 mitochondrial translational elongation
GO:0070126 mitochondrial translational termination

Cellular Component:
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005762 mitochondrial large ribosomal subunit
GO:0005840 ribosome
GO:0015934 large ribosomal subunit


-  Descriptions from all associated GenBank mRNAs
  AK000601 - Homo sapiens cDNA FLJ20594 fis, clone KAT08731.
AK302784 - Homo sapiens cDNA FLJ55384 complete cds, highly similar to Homo sapiens mitochondrial ribosomal protein L22 (MRPL22), transcript variant 1, mRNA.
CR933679 - Homo sapiens mRNA; cDNA DKFZp781F1071 (from clone DKFZp781F1071).
BC012565 - Homo sapiens mitochondrial ribosomal protein L22, mRNA (cDNA clone MGC:13429 IMAGE:4333117), complete cds.
AF161507 - Homo sapiens HSPC158 mRNA, complete cds.
CU680362 - Synthetic construct Homo sapiens gateway clone IMAGE:100018567 5' read MRPL22 mRNA.
HQ447930 - Synthetic construct Homo sapiens clone IMAGE:100071291; CCSB006413_03 mitochondrial ribosomal protein L22 (MRPL22) gene, encodes complete protein.
AK097880 - Homo sapiens cDNA FLJ40561 fis, clone THYMU2003855.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NWU5 (Reactome details) participates in the following event(s):

R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA
R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits
R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA
R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation)
R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome
R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP
R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA
R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA)
R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA
R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA
R-HSA-5368286 Mitochondrial translation initiation
R-HSA-5419276 Mitochondrial translation termination
R-HSA-5368287 Mitochondrial translation
R-HSA-5389840 Mitochondrial translation elongation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A6NGJ8, ENST00000523037.1, ENST00000523037.2, ENST00000523037.3, ENST00000523037.4, ENST00000523037.5, HSPC158, MRPL25, NM_014180, Q5H9Q1, Q96Q51, Q9NWU5, Q9P006, RM22_HUMAN, RPML25, uc323xgw.1, uc323xgw.2
UCSC ID: ENST00000523037.6_10
RefSeq Accession: NM_014180.4
Protein: Q9NWU5 (aka RM22_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.