Human Gene MRPS27 (ENST00000261413.10_8) from GENCODE V47lift37
  Description: mitochondrial ribosomal protein S27, transcript variant 2 (from RefSeq NM_015084.3)
Gencode Transcript: ENST00000261413.10_8
Gencode Gene: ENSG00000113048.19_13
Transcript (Including UTRs)
   Position: hg19 chr5:71,515,230-71,616,067 Size: 100,838 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr5:71,516,736-71,616,048 Size: 99,313 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:71,515,230-71,616,067)mRNA (may differ from genome)Protein (414 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: RT27_HUMAN
DESCRIPTION: RecName: Full=28S ribosomal protein S27, mitochondrial; Short=MRP-S27; Short=S27mt;
SUBUNIT: Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins. Interacts with NOA1.
SUBCELLULAR LOCATION: Mitochondrion.
SIMILARITY: Contains 2 PPR (pentatricopeptide) repeats.
SEQUENCE CAUTION: Sequence=BAA13394.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.84 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 698.77 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -3.0019-0.158 Picture PostScript Text
3' UTR -498.601506-0.331 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR002885 - Pentatricopeptide_repeat
IPR019266 - Ribosomal_S27_mit

Pfam Domains:
PF01535 - PPR repeat
PF10037 - Mitochondrial 28S ribosomal protein S27

SCOP Domains:
48371 - ARM repeat
46689 - Homeodomain-like

ModBase Predicted Comparative 3D Structure on Q92552
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000049 tRNA binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0019843 rRNA binding
GO:0097177 mitochondrial ribosome binding

Biological Process:
GO:0006417 regulation of translation
GO:0008283 cell proliferation
GO:0070125 mitochondrial translational elongation
GO:0070126 mitochondrial translational termination
GO:0070131 positive regulation of mitochondrial translation

Cellular Component:
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005743 mitochondrial inner membrane
GO:0005763 mitochondrial small ribosomal subunit
GO:0005840 ribosome


-  Descriptions from all associated GenBank mRNAs
  D87453 - Homo sapiens mRNA for KIAA0264 gene.
BC030521 - Homo sapiens mitochondrial ribosomal protein S27, mRNA (cDNA clone IMAGE:5189967), complete cds.
BC064902 - Homo sapiens mitochondrial ribosomal protein S27, mRNA (cDNA clone MGC:74772 IMAGE:6009616), complete cds.
BC011818 - Homo sapiens mitochondrial ribosomal protein S27, mRNA (cDNA clone IMAGE:3543162), with apparent retained intron.
AK025417 - Homo sapiens cDNA: FLJ21764 fis, clone COLF7036.
AK300111 - Homo sapiens cDNA FLJ53652 complete cds, highly similar to Mitochondrial 28S ribosomal protein S27.
AK299412 - Homo sapiens cDNA FLJ54536 complete cds, highly similar to Mitochondrial 28S ribosomal protein S27.
AB383826 - Synthetic construct DNA, clone: pF1KSDA0264, Homo sapiens MRPS27 gene for mitochondrial 28S ribosomal protein S27, complete cds, without stop codon, in Flexi system.
HQ448112 - Synthetic construct Homo sapiens clone IMAGE:100071493; CCSB012330_01 mitochondrial ribosomal protein S27 (MRPS27) gene, encodes complete protein.
KJ893327 - Synthetic construct Homo sapiens clone ccsbBroadEn_02721 MRPS27 gene, encodes complete protein.
CU687962 - Synthetic construct Homo sapiens gateway clone IMAGE:100021748 5' read MRPS27 mRNA.
AK309065 - Homo sapiens cDNA, FLJ99106.
BC003632 - Homo sapiens mitochondrial ribosomal protein S27, mRNA (cDNA clone IMAGE:3050209), partial cds.
AK027001 - Homo sapiens cDNA: FLJ23348 fis, clone HEP13748.
AK128683 - Homo sapiens cDNA FLJ46849 fis, clone UTERU3006538, highly similar to Mitochondrial 28S ribosomal protein S27.
JD146765 - Sequence 127789 from Patent EP1572962.
JD359314 - Sequence 340338 from Patent EP1572962.
JD347697 - Sequence 328721 from Patent EP1572962.
JD550402 - Sequence 531426 from Patent EP1572962.
JD154040 - Sequence 135064 from Patent EP1572962.
JD530656 - Sequence 511680 from Patent EP1572962.
JD065714 - Sequence 46738 from Patent EP1572962.
JD224140 - Sequence 205164 from Patent EP1572962.
JD299528 - Sequence 280552 from Patent EP1572962.
JD305839 - Sequence 286863 from Patent EP1572962.
JD363689 - Sequence 344713 from Patent EP1572962.
JD290727 - Sequence 271751 from Patent EP1572962.
JD310846 - Sequence 291870 from Patent EP1572962.
JD450234 - Sequence 431258 from Patent EP1572962.
JD208967 - Sequence 189991 from Patent EP1572962.
JD387373 - Sequence 368397 from Patent EP1572962.
JD214130 - Sequence 195154 from Patent EP1572962.
JD342697 - Sequence 323721 from Patent EP1572962.
JD182155 - Sequence 163179 from Patent EP1572962.
JD081621 - Sequence 62645 from Patent EP1572962.
JD414844 - Sequence 395868 from Patent EP1572962.
JD311823 - Sequence 292847 from Patent EP1572962.
JD069744 - Sequence 50768 from Patent EP1572962.
JD379692 - Sequence 360716 from Patent EP1572962.
JD414843 - Sequence 395867 from Patent EP1572962.
AK296581 - Homo sapiens cDNA FLJ54799 complete cds, highly similar to Mitochondrial 28S ribosomal protein S27.
JD344128 - Sequence 325152 from Patent EP1572962.
JD345838 - Sequence 326862 from Patent EP1572962.
JD193112 - Sequence 174136 from Patent EP1572962.
JD193183 - Sequence 174207 from Patent EP1572962.
JD095272 - Sequence 76296 from Patent EP1572962.
JD213015 - Sequence 194039 from Patent EP1572962.
JD542898 - Sequence 523922 from Patent EP1572962.
JD560940 - Sequence 541964 from Patent EP1572962.
JD262064 - Sequence 243088 from Patent EP1572962.
JD087062 - Sequence 68086 from Patent EP1572962.
JD328228 - Sequence 309252 from Patent EP1572962.
JD265875 - Sequence 246899 from Patent EP1572962.
JD063880 - Sequence 44904 from Patent EP1572962.
JD424961 - Sequence 405985 from Patent EP1572962.
JD497630 - Sequence 478654 from Patent EP1572962.
JD203913 - Sequence 184937 from Patent EP1572962.
JD339296 - Sequence 320320 from Patent EP1572962.
KJ902284 - Synthetic construct Homo sapiens clone ccsbBroadEn_11678 MRPS27 gene, encodes complete protein.
AK310474 - Homo sapiens cDNA, FLJ17516.
JD027270 - Sequence 8294 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q92552 (Reactome details) participates in the following event(s):

R-HSA-5368279 MTIF3 binds 28S ribosomal subunit
R-HSA-5419273 Hydrolysis of GTP and dissociation of 28S and 39S subunits
R-HSA-5389849 28S subunit:MTIF3 binds MTIF2:GTP, mRNA, and formylMet-tRNA
R-HSA-5389839 39S subunit binds 28S subunit:mRNA:fMet-tRNA
R-HSA-5389848 TUFM:GTP:aminoacyl-tRNA binds 55S ribosome:mRNA:fMet-tRNA
R-HSA-5389857 Peptide transfer from P-site to A-site (peptide bond formation)
R-HSA-5389842 TUFM hydrolyzes GTP and TUFM:GDP dissociates from 55S ribosome
R-HSA-5419261 55S ribosome with peptidyl-tRNA in A site binds GFM1:GTP
R-HSA-5419281 MRRF binds 55S ribosome:mRNA:tRNA
R-HSA-5419279 Translocation of peptidyl-tRNA from A-site to P-site (and translocation of 55S ribosome by 3 bases along mRNA)
R-HSA-5419264 MTRF1L (MTRF1a) or ICT1 binds stop codon in 55S ribosome:mRNA:peptidyl-tRNA
R-HSA-5419277 GFM2:GTP binds 55S ribosome:mRNA:tRNA:MRRF releasing mRNA and tRNA
R-HSA-5368286 Mitochondrial translation initiation
R-HSA-5419276 Mitochondrial translation termination
R-HSA-5368287 Mitochondrial translation
R-HSA-5389840 Mitochondrial translation elongation
R-HSA-72766 Translation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: B4DRT2, ENST00000261413.1, ENST00000261413.2, ENST00000261413.3, ENST00000261413.4, ENST00000261413.5, ENST00000261413.6, ENST00000261413.7, ENST00000261413.8, ENST00000261413.9, KIAA0264, MRPS27 , NM_015084, Q6P1S1, Q92552, RT27_HUMAN, uc317gpq.1, uc317gpq.2
UCSC ID: ENST00000261413.10_8
RefSeq Accession: NM_015084.3
Protein: Q92552 (aka RT27_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.