Human Gene MSI2 (ENST00000284073.7_5) from GENCODE V47lift37
  Description: musashi RNA binding protein 2, transcript variant 1 (from RefSeq NM_138962.4)
Gencode Transcript: ENST00000284073.7_5
Gencode Gene: ENSG00000153944.12_19
Transcript (Including UTRs)
   Position: hg19 chr17:55,333,884-55,762,050 Size: 428,167 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr17:55,334,104-55,754,389 Size: 420,286 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:55,333,884-55,762,050)mRNA (may differ from genome)Protein (328 aa)
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-  Comments and Description Text from UniProtKB
  ID: MSI2H_HUMAN
DESCRIPTION: RecName: Full=RNA-binding protein Musashi homolog 2; Short=Musashi-2;
FUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. May play a role in the proliferation and maintenance of stem cells in the central nervous system (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with polysomes (By similarity).
TISSUE SPECIFICITY: Ubiquitous; detected at low levels.
INDUCTION: Up-regulated in astrocytes after brain injury (By similarity).
PTM: Phosphorylated (By similarity).
DISEASE: Note=Chromosomal aberrations involving MSI2 may contribute to disease progression in chronic myeloid leukemia. Translocation t(7;17)(p15;q23) with HOXA9; translocation t(7;17)(q32-34;q23).
SIMILARITY: Belongs to the Musashi family.
SIMILARITY: Contains 2 RRM (RNA recognition motif) domains.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/MSI2ID42893ch17q23.html";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MSI2
Diseases sorted by gene-association score: b-cell adult acute lymphocytic leukemia (9)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 26.21 RPKM in Pituitary
Total median expression: 452.16 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -113.40220-0.515 Picture PostScript Text
3' UTR -1357.305172-0.262 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom

Pfam Domains:
PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

SCOP Domains:
50249 - Nucleic acid-binding proteins
54928 - RNA-binding domain, RBD

ModBase Predicted Comparative 3D Structure on Q96DH6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0003727 single-stranded RNA binding
GO:0005515 protein binding
GO:0008266 poly(U) RNA binding

Biological Process:
GO:0048864 stem cell development

Cellular Component:
GO:0005737 cytoplasm
GO:0005844 polysome


-  Descriptions from all associated GenBank mRNAs
  AK295060 - Homo sapiens cDNA FLJ56904 complete cds, highly similar to RNA-binding protein Musashi homolog 2.
BC001526 - Homo sapiens musashi homolog 2 (Drosophila), mRNA (cDNA clone MGC:3245 IMAGE:3505639), complete cds.
AB528970 - Synthetic construct DNA, clone: pF1KE0751, Homo sapiens MSI2 gene for musashi homolog 2, without stop codon, in Flexi system.
DQ892317 - Synthetic construct clone IMAGE:100004947; FLH185202.01X; RZPDo839G06146D musashi homolog 2 (Drosophila) (MSI2) gene, encodes complete protein.
DQ895519 - Synthetic construct Homo sapiens clone IMAGE:100009979; FLH185198.01L; RZPDo839G06145D musashi homolog 2 (Drosophila) (MSI2) gene, encodes complete protein.
KJ900089 - Synthetic construct Homo sapiens clone ccsbBroadEn_09483 MSI2 gene, encodes complete protein.
AK093888 - Homo sapiens cDNA FLJ36569 fis, clone TRACH2010824, highly similar to Ribonucleoprotein.
AX748353 - Sequence 1878 from Patent EP1308459.
AK297295 - Homo sapiens cDNA FLJ52888 complete cds, highly similar to RNA-binding protein Musashi homolog 2.
BC017560 - Homo sapiens musashi homolog 2 (Drosophila), mRNA (cDNA clone IMAGE:3922563), complete cds.
KJ903713 - Synthetic construct Homo sapiens clone ccsbBroadEn_13107 MSI2 gene, encodes complete protein.
BX647939 - Homo sapiens mRNA; cDNA DKFZp686H1624 (from clone DKFZp686H1624).
JD522830 - Sequence 503854 from Patent EP1572962.
JD471451 - Sequence 452475 from Patent EP1572962.
BX647282 - Homo sapiens mRNA; cDNA DKFZp686A0493 (from clone DKFZp686A0493).
JD414067 - Sequence 395091 from Patent EP1572962.
JD319266 - Sequence 300290 from Patent EP1572962.
JD105041 - Sequence 86065 from Patent EP1572962.
JD078179 - Sequence 59203 from Patent EP1572962.
JD148059 - Sequence 129083 from Patent EP1572962.
JD566750 - Sequence 547774 from Patent EP1572962.
JD555032 - Sequence 536056 from Patent EP1572962.
JD519516 - Sequence 500540 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000284073.1, ENST00000284073.2, ENST00000284073.3, ENST00000284073.4, ENST00000284073.5, ENST00000284073.6, MSI2H_HUMAN, NM_138962, Q7Z6M7, Q8N9T4, Q96DH6, uc317kdz.1, uc317kdz.2
UCSC ID: ENST00000284073.7_5
RefSeq Accession: NM_138962.4
Protein: Q96DH6 (aka MSI2H_HUMAN or MSI2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.