Human Gene MSLN (ENST00000545450.7_7) from GENCODE V47lift37
  Description: mesothelin, transcript variant 1 (from RefSeq NM_005823.6)
Gencode Transcript: ENST00000545450.7_7
Gencode Gene: ENSG00000102854.16_11
Transcript (Including UTRs)
   Position: hg19 chr16:810,734-818,862 Size: 8,129 Total Exon Count: 18 Strand: +
Coding Region
   Position: hg19 chr16:812,681-818,733 Size: 6,053 Coding Exon Count: 16 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:810,734-818,862)mRNA (may differ from genome)Protein (622 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MSLN_HUMAN
DESCRIPTION: RecName: Full=Mesothelin; AltName: Full=CAK1 antigen; AltName: Full=Pre-pro-megakaryocyte-potentiating factor; Contains: RecName: Full=Megakaryocyte-potentiating factor; Short=MPF; Contains: RecName: Full=Mesothelin, cleaved form; Flags: Precursor;
FUNCTION: Membrane-anchored forms may play a role in cellular adhesion.
FUNCTION: Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
SUBUNIT: Interacts with MUC16.
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.
SUBCELLULAR LOCATION: Megakaryocyte-potentiating factor: Secreted.
SUBCELLULAR LOCATION: Isoform 3: Secreted.
TISSUE SPECIFICITY: Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
PTM: Both MPF and the cleaved form of mesothelin are N- glycosylated.
PTM: Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
DISEASE: Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
SIMILARITY: Belongs to the mesothelin family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: MSLN
Diseases sorted by gene-association score: malignant pleural mesothelioma (10), pancreatic cancer (9), ovarian cancer, somatic (6), mesothelioma, somatic (5), malignant biphasic mesothelioma (4), benign mesothelioma (4), pancreatic ductal adenocarcinoma (4), triple-receptor negative breast cancer (3), lung cancer susceptibility 3 (3), refractory hematologic cancer (2), pleural cancer (2), renal clear cell carcinoma (2), refractory hairy cell leukemia (2), connective tissue cancer (2), pancreas adenocarcinoma (2), colloid carcinoma of the pancreas (1), malignant ovarian surface epithelial-stromal neoplasm (1), ovary epithelial cancer (1), pericardium cancer (1), female reproductive organ cancer (1), pericardial mesothelioma (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 31.97 RPKM in Lung
Total median expression: 100.79 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -74.70199-0.375 Picture PostScript Text
3' UTR -51.30129-0.398 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR010335 - Mesothelin
IPR026664 - Stereocilin_related

Pfam Domains:
PF06060 - Pre-pro-megakaryocyte potentiating factor precursor (Mesothelin)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
4F3F - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q13421
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding

Biological Process:
GO:0007155 cell adhesion
GO:0007160 cell-matrix adhesion
GO:0031016 pancreas development
GO:0043687 post-translational protein modification
GO:0044267 cellular protein metabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space
GO:0005788 endoplasmic reticulum lumen
GO:0005794 Golgi apparatus
GO:0005886 plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0031225 anchored component of membrane


-  Descriptions from all associated GenBank mRNAs
  EF420155 - Homo sapiens mesothelin (MSLN) mRNA, 5' UTR.
AY743922 - Homo sapiens mesothelin precursor (MSLN) mRNA, complete cds.
BC009272 - Homo sapiens mesothelin, mRNA (cDNA clone MGC:10273 IMAGE:3957372), complete cds.
BC003512 - Homo sapiens mesothelin, mRNA (cDNA clone MGC:10686 IMAGE:3611296), complete cds.
JD276402 - Sequence 257426 from Patent EP1572962.
U40434 - Human mesothelin or CAK1 antigen precursor mRNA, complete cds.
D49441 - Homo sapiens mRNA for pre-pro-megakaryocyte potentiating factor, complete cds.
E07943 - cDNA encoding a polypeptide having megakaryocyte potentiating activity.
DQ891948 - Synthetic construct clone IMAGE:100004578; FLH181685.01X; RZPDo839G01136D mesothelin (MSLN) gene, encodes complete protein.
EU176658 - Synthetic construct Homo sapiens clone IMAGE:100011451; FLH181684.01L; RZPDo839H11254D mesothelin (MSLN) gene, encodes complete protein.
KJ904646 - Synthetic construct Homo sapiens clone ccsbBroadEn_14040 MSLN gene, encodes complete protein.
AF180951 - Homo sapiens mesothelin/megakaryocyte potentiating factor mRNA, partial cds.
JD130652 - Sequence 111676 from Patent EP1572962.
JD394242 - Sequence 375266 from Patent EP1572962.
JD474031 - Sequence 455055 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q13421 (Reactome details) participates in the following event(s):

R-HSA-8940388 GPLD1 hydrolyses GPI-anchors from proteins
R-HSA-8952289 FAM20C phosphorylates FAM20C substrates
R-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
R-HSA-597592 Post-translational protein modification
R-HSA-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-HSA-8957275 Post-translational protein phosphorylation
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: D3DU65, ENST00000545450.1, ENST00000545450.2, ENST00000545450.3, ENST00000545450.4, ENST00000545450.5, ENST00000545450.6, MPF, MSLN_HUMAN, NM_005823, Q13421, Q14859, Q4VQD5, Q96GR6, Q96KJ5, Q9BR17, Q9BTR2, Q9UCB2, Q9UK57, uc324wld.1, uc324wld.2
UCSC ID: ENST00000545450.7_7
RefSeq Accession: NM_005823.6
Protein: Q13421 (aka MSLN_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.