Human Gene MYL6B (ENST00000695999.1_3) from GENCODE V47lift37

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Description: myosin light chain 6B, transcript variant 2 (from RefSeq NM_002475.5)
Gencode Transcript: ENST00000695999.1_3
Gencode Gene: ENSG00000196465.11_12
Transcript (Including UTRs)
Position: hg19 chr12:56,546,371-56,551,766 Size: 5,396 Total Exon Count: 8 Strand: +
Coding Region
Position: hg19 chr12:56,547,703-56,551,510 Size: 3,808 Coding Exon Count: 6

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations	mRNA Descriptions
Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr12:56,546,371-56,551,766)			mRNA (may differ from genome)		Protein (208 aa)
Gene Sorter	Genome Browser	Other Species FASTA	Gene interactions	Table Schema	AlphaFold
BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards	HGNC
MGI	OMIM	PubMed	Reactome	UniProtKB	Wikipedia
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: MYL6B_HUMAN DESCRIPTION: RecName: Full=Myosin light chain 6B; AltName: Full=Myosin light chain 1 slow-twitch muscle A isoform; Short=MLC1sa; AltName: Full=Smooth muscle and nonmuscle myosin light chain alkali 6B; FUNCTION: Regulatory light chain of myosin. Does not bind calcium. SUBUNIT: Myosin is a hexamer of 2 heavy chains and 4 light chains. INTERACTION: Q02440:MYO5A (xeno); NbExp=2; IntAct=EBI-358570, EBI-1040586; SIMILARITY: Contains 3 EF-hand domains.

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene C049325 1,2-dithiol-3-thione D001564 Benzo(a)pyrene D019327 Copper Sulfate D003993 Dibutyl Phthalate D004237 Diuron D006830 Hydralazine D015741 Metribolone D010634 Phenobarbital D013749 Tetrachlorodibenzodioxin D013759 Tetrahydrocannabinol more ... click here to view the complete list

Common Gene Haplotype Alleles


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Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-19.90	91	-0.219	Picture	PostScript	Text
3' UTR	-44.50	110	-0.405	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR011992 - EF-hand-like_dom
IPR018249 - EF_HAND_2
IPR002048 - EF_hand_Ca-bd

SCOP Domains:
47473 - EF-hand

Protein Data Bank (PDB) 3-D Structure
MuPIT help

1OE9 - X-ray MuPIT

1W7I - X-ray MuPIT

1W7J - X-ray MuPIT

ModBase Predicted Comparative 3D Structure on P14649


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0003774 motor activity GO:0005509 calcium ion binding GO:0005515 protein binding GO:0008307 structural constituent of muscle Biological Process: GO:0006936 muscle contraction GO:0007519 skeletal muscle tissue development GO:0030049 muscle filament sliding Cellular Component: GO:0005829 cytosol GO:0005859 muscle myosin complex GO:0016459 myosin complex GO:0016461 unconventional myosin complex GO:0070062 extracellular exosome

Descriptions from all associated GenBank mRNAs


	BC014400 - Homo sapiens myosin, light chain 6B, alkali, smooth muscle and non-muscle, mRNA (cDNA clone MGC:15776 IMAGE:3503030), complete cds. AK304594 - Homo sapiens cDNA FLJ60058 complete cds, highly similar to Myosin light chain 1, slow-twitch muscle A isoform. AK310996 - Homo sapiens cDNA, FLJ18038. BC012425 - Homo sapiens myosin, light chain 6B, alkali, smooth muscle and non-muscle, mRNA (cDNA clone MGC:9212 IMAGE:3862144), complete cds. M31211 - Human myosin light chain 1 slow a (MLC1sa) mRNA, complete cds. X16434 - MYOSIN ALKALI LIGHT CHAIN. KJ900193 - Synthetic construct Homo sapiens clone ccsbBroadEn_09587 MYL6B gene, encodes complete protein. DQ891306 - Synthetic construct clone IMAGE:100003936; FLH170941.01X; RZPDo839D04100D myosin, light polypeptide 6B, alkali, smooth muscle and non-muscle (MYL6B) gene, encodes complete protein. DQ894491 - Synthetic construct Homo sapiens clone IMAGE:100008951; FLH170937.01L; RZPDo839D0499D myosin, light polypeptide 6B, alkali, smooth muscle and non-muscle (MYL6B) gene, encodes complete protein. JD281654 - Sequence 262678 from Patent EP1572962. LF317691 - JP 2014500723-A/125194: Polycomb-Associated Non-Coding RNAs. JD557138 - Sequence 538162 from Patent EP1572962. JD128497 - Sequence 109521 from Patent EP1572962. MA553268 - JP 2018138019-A/125194: Polycomb-Associated Non-Coding RNAs.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein P14649 (Reactome details) participates in the following event(s): R-HSA-445813 Phosphorylation of Smooth Muscle Myosin Light Chains R-HSA-445700 Myosin Binds ATP R-HSA-445704 Calcium Binds Caldesmon R-HSA-445705 Release Of ADP From Myosin R-HSA-445699 ATP Hydrolysis By Myosin R-HSA-445355 Smooth Muscle Contraction R-HSA-397014 Muscle contraction

Other Names for This Gene


	Alternate Gene Symbols: MLC1SA, MYL6B_HUMAN, NM_002475, P14649, uc331aii.1, uc331aii.2 UCSC ID: ENST00000695999.1_3 RefSeq Accession: NM_002475.5 Protein: P14649 (aka MYL6B_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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