Human Gene MYLK4 (ENST00000274643.9_10) from GENCODE V47lift37
  Description: myosin light chain kinase family member 4, transcript variant 1 (from RefSeq NM_001012418.5)
Gencode Transcript: ENST00000274643.9_10
Gencode Gene: ENSG00000145949.12_15
Transcript (Including UTRs)
   Position: hg19 chr6:2,663,871-2,751,156 Size: 87,286 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chr6:2,671,535-2,749,528 Size: 77,994 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr6:2,663,871-2,751,156)mRNA (may differ from genome)Protein (388 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MYLK4_HUMAN
DESCRIPTION: RecName: Full=Myosin light chain kinase family member 4; EC=2.7.11.1; AltName: Full=Sugen kinase 85; Short=SgK085;
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SIMILARITY: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.70 RPKM in Muscle - Skeletal
Total median expression: 28.82 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -79.30299-0.265 Picture PostScript Text
3' UTR -1299.304288-0.303 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR020636 - Ca/CaM-dep_Ca-dep_prot_Kinase
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF03109 - ABC1 atypical kinase-like domain
PF06293 - Lipopolysaccharide kinase (Kdo/WaaP) family
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
56112 - Protein kinase-like (PK-like)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2X4F - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q86YV6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0004687 myosin light chain kinase activity
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0018107 peptidyl-threonine phosphorylation
GO:0035556 intracellular signal transduction

Cellular Component:
GO:0005622 intracellular


-  Descriptions from all associated GenBank mRNAs
  AK127360 - Homo sapiens cDNA FLJ45434 fis, clone BRHIP3041587, weakly similar to Myosin light chain kinase, skeletal muscle (EC 2.7.1.117).
BX647582 - Homo sapiens mRNA; cDNA DKFZp451H166 (from clone DKFZp451H166).
AK090753 - Homo sapiens cDNA FLJ33434 fis, clone BRACE2021264.
AK122581 - Homo sapiens mRNA for FLJ00265 protein.
JD287605 - Sequence 268629 from Patent EP1572962.
JD058927 - Sequence 39951 from Patent EP1572962.
JD355966 - Sequence 336990 from Patent EP1572962.
JD158411 - Sequence 139435 from Patent EP1572962.
JD507710 - Sequence 488734 from Patent EP1572962.
JD410315 - Sequence 391339 from Patent EP1572962.
JD494208 - Sequence 475232 from Patent EP1572962.
JD046839 - Sequence 27863 from Patent EP1572962.
JD182972 - Sequence 163996 from Patent EP1572962.
JD268126 - Sequence 249150 from Patent EP1572962.
JD182971 - Sequence 163995 from Patent EP1572962.
JD268125 - Sequence 249149 from Patent EP1572962.
JD276559 - Sequence 257583 from Patent EP1572962.
JD290136 - Sequence 271160 from Patent EP1572962.
JD373986 - Sequence 355010 from Patent EP1572962.
JD433964 - Sequence 414988 from Patent EP1572962.
JD292245 - Sequence 273269 from Patent EP1572962.
JD464205 - Sequence 445229 from Patent EP1572962.
JD399566 - Sequence 380590 from Patent EP1572962.
JD124765 - Sequence 105789 from Patent EP1572962.
JD267863 - Sequence 248887 from Patent EP1572962.
JD249468 - Sequence 230492 from Patent EP1572962.
JD266868 - Sequence 247892 from Patent EP1572962.
JD352708 - Sequence 333732 from Patent EP1572962.
JD352707 - Sequence 333731 from Patent EP1572962.
JD562391 - Sequence 543415 from Patent EP1572962.
JD439838 - Sequence 420862 from Patent EP1572962.
JD184417 - Sequence 165441 from Patent EP1572962.
JD304451 - Sequence 285475 from Patent EP1572962.
JD402369 - Sequence 383393 from Patent EP1572962.
JD358875 - Sequence 339899 from Patent EP1572962.
JD040126 - Sequence 21150 from Patent EP1572962.
JD499220 - Sequence 480244 from Patent EP1572962.
JD347923 - Sequence 328947 from Patent EP1572962.
JD331566 - Sequence 312590 from Patent EP1572962.
JD505561 - Sequence 486585 from Patent EP1572962.
JD345641 - Sequence 326665 from Patent EP1572962.
JD345642 - Sequence 326666 from Patent EP1572962.
JD374535 - Sequence 355559 from Patent EP1572962.
JD527577 - Sequence 508601 from Patent EP1572962.
JD153124 - Sequence 134148 from Patent EP1572962.
JD198393 - Sequence 179417 from Patent EP1572962.
JD140614 - Sequence 121638 from Patent EP1572962.
JD317788 - Sequence 298812 from Patent EP1572962.
JD456340 - Sequence 437364 from Patent EP1572962.
JD539200 - Sequence 520224 from Patent EP1572962.
JD185221 - Sequence 166245 from Patent EP1572962.
JD053701 - Sequence 34725 from Patent EP1572962.
JD507993 - Sequence 489017 from Patent EP1572962.
BC132831 - Homo sapiens myosin light chain kinase family, member 4, mRNA (cDNA clone MGC:164462 IMAGE:40146853), complete cds.
BC132833 - Homo sapiens myosin light chain kinase family, member 4, mRNA (cDNA clone MGC:164464 IMAGE:40146855), complete cds.
JD318640 - Sequence 299664 from Patent EP1572962.
JD528884 - Sequence 509908 from Patent EP1572962.
JD296844 - Sequence 277868 from Patent EP1572962.
JD551378 - Sequence 532402 from Patent EP1572962.
JD179242 - Sequence 160266 from Patent EP1572962.
JD250877 - Sequence 231901 from Patent EP1572962.
JD315716 - Sequence 296740 from Patent EP1572962.
JD540243 - Sequence 521267 from Patent EP1572962.
JD445436 - Sequence 426460 from Patent EP1572962.
JD456348 - Sequence 437372 from Patent EP1572962.
JD126058 - Sequence 107082 from Patent EP1572962.
JD137742 - Sequence 118766 from Patent EP1572962.
JD370765 - Sequence 351789 from Patent EP1572962.
JD264121 - Sequence 245145 from Patent EP1572962.
KJ900629 - Synthetic construct Homo sapiens clone ccsbBroadEn_10023 MYLK4 gene, encodes complete protein.
AB528897 - Synthetic construct DNA, clone: pF1KE0656, Homo sapiens MYLK4 gene for myosin light chain kinase family, member 4, without stop codon, in Flexi system.
AK096893 - Homo sapiens cDNA FLJ39574 fis, clone SKMUS2002693.

-  Other Names for This Gene
  Alternate Gene Symbols: A2RUC0, ENST00000274643.1, ENST00000274643.2, ENST00000274643.3, ENST00000274643.4, ENST00000274643.5, ENST00000274643.6, ENST00000274643.7, ENST00000274643.8, MYLK4_HUMAN, NM_001012418, Q5TAW2, Q86YV6, SGK085, uc317jfy.1, uc317jfy.2
UCSC ID: ENST00000274643.9_10
RefSeq Accession: NM_001012418.5
Protein: Q86YV6 (aka MYLK4_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.