Human Gene MYOCD (ENST00000425538.6_8) from GENCODE V47lift37
  Description: myocardin, transcript variant 3 (from RefSeq NM_001378306.1)
Gencode Transcript: ENST00000425538.6_8
Gencode Gene: ENSG00000141052.18_10
Transcript (Including UTRs)
   Position: hg19 chr17:12,569,207-12,672,266 Size: 103,060 Total Exon Count: 14 Strand: +
Coding Region
   Position: hg19 chr17:12,569,506-12,666,961 Size: 97,456 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:12,569,207-12,672,266)mRNA (may differ from genome)Protein (986 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MYCD_HUMAN
DESCRIPTION: RecName: Full=Myocardin;
FUNCTION: Smooth muscle cells (SM) and cardiac muscle cells- specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).
SUBUNIT: Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C- terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).
INTERACTION: P11831:SRF; NbExp=2; IntAct=EBI-493384, EBI-493034;
SUBCELLULAR LOCATION: Nucleus (By similarity).
TISSUE SPECIFICITY: Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.
INDUCTION: Up-regulated during heart failure.
DOMAIN: The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).
PTM: Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity (By similarity).
SIMILARITY: Contains 3 RPEL repeats.
SIMILARITY: Contains 1 SAP domain.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 21.62 RPKM in Colon - Sigmoid
Total median expression: 188.21 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -129.60299-0.433 Picture PostScript Text
3' UTR -1441.605305-0.272 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004018 - RPEL_repeat
IPR003034 - SAP_DNA-bd

Pfam Domains:
PF02037 - SAP domain
PF02755 - RPEL repeat

SCOP Domains:
68906 - SAP domain

ModBase Predicted Comparative 3D Structure on Q8IZQ8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000987 core promoter proximal region sequence-specific DNA binding
GO:0001046 core promoter sequence-specific DNA binding
GO:0001076 transcription factor activity, RNA polymerase II transcription factor binding
GO:0001077 transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
GO:0001105 RNA polymerase II transcription coactivator activity
GO:0001228 transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
GO:0003713 transcription coactivator activity
GO:0005515 protein binding
GO:0008134 transcription factor binding
GO:0035035 histone acetyltransferase binding
GO:0042826 histone deacetylase binding
GO:0061629 RNA polymerase II sequence-specific DNA binding transcription factor binding
GO:0070412 R-SMAD binding

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0001560 regulation of cell growth by extracellular stimulus
GO:0001570 vasculogenesis
GO:0001666 response to hypoxia
GO:0003231 cardiac ventricle development
GO:0003257 positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter
GO:0006366 transcription from RNA polymerase II promoter
GO:0007507 heart development
GO:0008284 positive regulation of cell proliferation
GO:0008285 negative regulation of cell proliferation
GO:0010628 positive regulation of gene expression
GO:0010667 negative regulation of cardiac muscle cell apoptotic process
GO:0010832 negative regulation of myotube differentiation
GO:0030511 positive regulation of transforming growth factor beta receptor signaling pathway
GO:0035051 cardiocyte differentiation
GO:0035065 regulation of histone acetylation
GO:0035886 vascular smooth muscle cell differentiation
GO:0042692 muscle cell differentiation
GO:0043388 positive regulation of DNA binding
GO:0043954 cellular component maintenance
GO:0045661 regulation of myoblast differentiation
GO:0045736 negative regulation of cyclin-dependent protein serine/threonine kinase activity
GO:0045893 positive regulation of transcription, DNA-templated
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0045987 positive regulation of smooth muscle contraction
GO:0048286 lung alveolus development
GO:0048565 digestive tract development
GO:0051091 positive regulation of sequence-specific DNA binding transcription factor activity
GO:0051145 smooth muscle cell differentiation
GO:0051150 regulation of smooth muscle cell differentiation
GO:0051152 positive regulation of smooth muscle cell differentiation
GO:0055007 cardiac muscle cell differentiation
GO:0055012 ventricular cardiac muscle cell differentiation
GO:0060065 uterus development
GO:0060157 urinary bladder development
GO:0097070 ductus arteriosus closure
GO:1900222 negative regulation of beta-amyloid clearance
GO:1900239 regulation of phenotypic switching
GO:1901228 positive regulation of transcription from RNA polymerase II promoter involved in heart development
GO:1904706 negative regulation of vascular smooth muscle cell proliferation
GO:1904753 negative regulation of vascular associated smooth muscle cell migration
GO:2000587 negative regulation of platelet-derived growth factor receptor-beta signaling pathway
GO:2000721 positive regulation of transcription from RNA polymerase II promoter involved in smooth muscle cell differentiation
GO:2000724 positive regulation of cardiac vascular smooth muscle cell differentiation
GO:2000727 positive regulation of cardiac muscle cell differentiation
GO:2001015 negative regulation of skeletal muscle cell differentiation

Cellular Component:
GO:0000790 nuclear chromatin
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AY764180 - Homo sapiens myocardin mRNA, complete cds, alternatively spliced.
AK128608 - Homo sapiens cDNA FLJ46767 fis, clone TRACH3024823.
AK292885 - Homo sapiens cDNA FLJ78477 complete cds, highly similar to Homo sapiens myocardin mRNA, complete cds.
BC126307 - Homo sapiens myocardin, mRNA (cDNA clone MGC:161585 IMAGE:8992023), complete cds.
BC143391 - Homo sapiens cDNA clone MGC:176914 IMAGE:9051897, complete cds.
KJ899966 - Synthetic construct Homo sapiens clone ccsbBroadEn_09360 MYOCD gene, encodes complete protein.
AF532596 - Homo sapiens myocardin transcription factor mRNA, complete cds.
AK092562 - Homo sapiens cDNA FLJ35243 fis, clone PROST2002685.
AK097821 - Homo sapiens cDNA FLJ40502 fis, clone TESTI2045199.
BX640673 - Homo sapiens mRNA; cDNA DKFZp686O15128 (from clone DKFZp686O15128).
AK095846 - Homo sapiens cDNA FLJ38527 fis, clone HCHON2000935.
JD056081 - Sequence 37105 from Patent EP1572962.
JD091203 - Sequence 72227 from Patent EP1572962.
JD048845 - Sequence 29869 from Patent EP1572962.
JD061043 - Sequence 42067 from Patent EP1572962.
JD291233 - Sequence 272257 from Patent EP1572962.
JD048813 - Sequence 29837 from Patent EP1572962.
JD188173 - Sequence 169197 from Patent EP1572962.
JD412192 - Sequence 393216 from Patent EP1572962.
JD467880 - Sequence 448904 from Patent EP1572962.
JD245611 - Sequence 226635 from Patent EP1572962.
JD301918 - Sequence 282942 from Patent EP1572962.
JD133693 - Sequence 114717 from Patent EP1572962.
JD251245 - Sequence 232269 from Patent EP1572962.
AK096753 - Homo sapiens cDNA FLJ39434 fis, clone PROST2004161.
JD547362 - Sequence 528386 from Patent EP1572962.
JD248087 - Sequence 229111 from Patent EP1572962.
JD553448 - Sequence 534472 from Patent EP1572962.
JD553068 - Sequence 534092 from Patent EP1572962.
JD566712 - Sequence 547736 from Patent EP1572962.
JD061586 - Sequence 42610 from Patent EP1572962.
JD196705 - Sequence 177729 from Patent EP1572962.
JD181696 - Sequence 162720 from Patent EP1572962.
JD331421 - Sequence 312445 from Patent EP1572962.
JD183110 - Sequence 164134 from Patent EP1572962.
JD090507 - Sequence 71531 from Patent EP1572962.
JD050607 - Sequence 31631 from Patent EP1572962.
JD098942 - Sequence 79966 from Patent EP1572962.
JD509772 - Sequence 490796 from Patent EP1572962.
JD467196 - Sequence 448220 from Patent EP1572962.
JD306617 - Sequence 287641 from Patent EP1572962.
JD204332 - Sequence 185356 from Patent EP1572962.
JD343043 - Sequence 324067 from Patent EP1572962.
JD204383 - Sequence 185407 from Patent EP1572962.
JD434759 - Sequence 415783 from Patent EP1572962.
JD038145 - Sequence 19169 from Patent EP1572962.
JD343281 - Sequence 324305 from Patent EP1572962.
JD343275 - Sequence 324299 from Patent EP1572962.
JD343205 - Sequence 324229 from Patent EP1572962.
JD343273 - Sequence 324297 from Patent EP1572962.
JD219161 - Sequence 200185 from Patent EP1572962.
JD221299 - Sequence 202323 from Patent EP1572962.
JD311133 - Sequence 292157 from Patent EP1572962.
JD313645 - Sequence 294669 from Patent EP1572962.
JD488273 - Sequence 469297 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000425538.1, ENST00000425538.2, ENST00000425538.3, ENST00000425538.4, ENST00000425538.5, MYCD, MYCD_HUMAN, NM_001378306, Q5UBU5, Q8IZQ8, Q8N7Q1, uc319utg.1, uc319utg.2
UCSC ID: ENST00000425538.6_8
RefSeq Accession: NM_001146312.3
Protein: Q8IZQ8 (aka MYCD_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.