Human Gene NAALADL1 (ENST00000358658.8_10) from GENCODE V47lift37
  Description: N-acetylated alpha-linked acidic dipeptidase like 1 (from RefSeq NM_005468.3)
Gencode Transcript: ENST00000358658.8_10
Gencode Gene: ENSG00000168060.16_17
Transcript (Including UTRs)
   Position: hg19 chr11:64,812,290-64,826,025 Size: 13,736 Total Exon Count: 18 Strand: -
Coding Region
   Position: hg19 chr11:64,812,743-64,825,993 Size: 13,251 Coding Exon Count: 18 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:64,812,290-64,826,025)mRNA (may differ from genome)Protein (740 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NALDL_HUMAN
DESCRIPTION: RecName: Full=N-acetylated-alpha-linked acidic dipeptidase-like protein; Short=NAALADase L; EC=3.4.17.21; AltName: Full=100 kDa ileum brush border membrane protein; Short=I100; AltName: Full=Ileal dipeptidylpeptidase;
FUNCTION: NAALADase-like activity unknown. Has no NAAG hydrolyzing activity. Exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC (By similarity).
CATALYTIC ACTIVITY: Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma- glutamates.
COFACTOR: Binds 2 zinc ions per subunit (By similarity).
SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type II membrane protein. Note=Ileal brush border membrane.
TISSUE SPECIFICITY: Mainly expressed in the distal small intestine. Also expressed in the spleen and testis. Weak expression in the brain, locating mainly to the brain stem, amygdala, thalamus and ventral striatum. Isoform 2 and 3 isoform 3 are found in the small intestine and colon.
SIMILARITY: Belongs to the peptidase M28 family. M28B subfamily.
SEQUENCE CAUTION: Sequence=AAB87645.1; Type=Frameshift; Positions=686, 693;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 45.31 RPKM in Small Intestine - Terminal Ileum
Total median expression: 109.29 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -4.9032-0.153 Picture PostScript Text
3' UTR -170.70453-0.377 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR007484 - Peptidase_M28
IPR003137 - Protease-assoc_domain
IPR007365 - TFR-like_dimer_dom

Pfam Domains:
PF02225 - PA domain
PF04253 - Transferrin receptor-like dimerisation domain
PF04389 - Peptidase family M28

SCOP Domains:
47672 - Transferrin receptor-like dimerisation domain
53187 - Zn-dependent exopeptidases
52025 - PA domain

ModBase Predicted Comparative 3D Structure on Q9UQQ1
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0004180 carboxypeptidase activity
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0016787 hydrolase activity
GO:0016805 dipeptidase activity
GO:0046872 metal ion binding

Biological Process:
GO:0006508 proteolysis
GO:0008152 metabolic process

Cellular Component:
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0016324 apical plasma membrane


-  Descriptions from all associated GenBank mRNAs
  LF211188 - JP 2014500723-A/18691: Polycomb-Associated Non-Coding RNAs.
LF211189 - JP 2014500723-A/18692: Polycomb-Associated Non-Coding RNAs.
AF010141 - Homo sapiens peptidase homolog mRNA, partial cds.
AJ012371 - Homo sapiens mRNA for NAALADase L protein.
JD104728 - Sequence 85752 from Patent EP1572962.
BC143643 - Homo sapiens cDNA clone IMAGE:9052157, with apparent retained intron.
BC143644 - Homo sapiens cDNA clone IMAGE:9052158, containing frame-shift errors.
JD497916 - Sequence 478940 from Patent EP1572962.
JD477600 - Sequence 458624 from Patent EP1572962.
JD392536 - Sequence 373560 from Patent EP1572962.
BC140321 - Synthetic construct Homo sapiens clone IMAGE:100014307, MGC:173183 N-acetylated alpha-linked acidic dipeptidase-like 1 (NAALADL1) mRNA, encodes complete protein.
AB527189 - Synthetic construct DNA, clone: pF1KE0037, Homo sapiens NAALADL1 gene for N-acetylated alpha-linked acidic dipeptidase-like 1, without stop codon, in Flexi system.
AF001547 - Homo sapiens clone gamma mRNA sequence.
AK311090 - Homo sapiens cDNA, FLJ18132.
AK309536 - Homo sapiens cDNA, FLJ99577.
LF344492 - JP 2014500723-A/151995: Polycomb-Associated Non-Coding RNAs.
LF344491 - JP 2014500723-A/151994: Polycomb-Associated Non-Coding RNAs.
LF344490 - JP 2014500723-A/151993: Polycomb-Associated Non-Coding RNAs.
MA580069 - JP 2018138019-A/151995: Polycomb-Associated Non-Coding RNAs.
MA580068 - JP 2018138019-A/151994: Polycomb-Associated Non-Coding RNAs.
MA580067 - JP 2018138019-A/151993: Polycomb-Associated Non-Coding RNAs.
MA446765 - JP 2018138019-A/18691: Polycomb-Associated Non-Coding RNAs.
MA446766 - JP 2018138019-A/18692: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: C9J8A1, C9J964, C9JL35, C9JSN0, ENST00000358658.1, ENST00000358658.2, ENST00000358658.3, ENST00000358658.4, ENST00000358658.5, ENST00000358658.6, ENST00000358658.7, NAALADASEL, NAALADL , NALDL_HUMAN, NM_005468, O43176, Q9UQQ1, uc318ara.1, uc318ara.2
UCSC ID: ENST00000358658.8_10
RefSeq Accession: NM_005468.3
Protein: Q9UQQ1 (aka NALDL_HUMAN)

-  Gene Model Information
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-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.