Human Gene NANOGP8 (ENST00000528386.4_10) from GENCODE V47lift37
  Description: Nanog homeobox retrogene P8 (from RefSeq NM_001355281.2)
Gencode Transcript: ENST00000528386.4_10
Gencode Gene: ENSG00000255192.7_15
Transcript (Including UTRs)
   Position: hg19 chr15:35,375,611-35,377,496 Size: 1,886 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr15:35,376,394-35,377,311 Size: 918 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
RNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA DescriptionsOther Names
Model InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr15:35,375,611-35,377,496)mRNA (may differ from genome)Protein (305 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NANP8_HUMAN
DESCRIPTION: RecName: Full=Putative homeobox protein NANOGP8;
FUNCTION: May act as a transcription regulator (By similarity). When overexpressed, promotes entry of cells into S phase and cell proliferation.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed in osteosarcoma cancer cell line (at protein level). breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines.
MISCELLANEOUS: Almost identical to NANOG. There is only one change in the inferred amino acid sequence from 'Gln-253' in NANOG to His-253 in NANOGP8.
SIMILARITY: Belongs to the Nanog homeobox family.
SIMILARITY: Contains 1 homeobox DNA-binding domain.
CAUTION: Could be the product of a pseudogene.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 0.59 RPKM in Testis
Total median expression: 3.78 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -37.20185-0.201 Picture PostScript Text
3' UTR -240.00783-0.307 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR017970 - Homeobox_CS
IPR001356 - Homeodomain
IPR009057 - Homeodomain-like

Pfam Domains:
PF00046 - Homeodomain

SCOP Domains:
46689 - Homeodomain-like

ModBase Predicted Comparative 3D Structure on Q6NSW7
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0003677 DNA binding
GO:0043565 sequence-specific DNA binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0008284 positive regulation of cell proliferation
GO:1902808 positive regulation of cell cycle G1/S phase transition

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  JD565599 - Sequence 546623 from Patent EP1572962.
BC069807 - Homo sapiens Nanog homeobox pseudogene 8, mRNA (cDNA clone IMAGE:7262621).
JD063198 - Sequence 44222 from Patent EP1572962.
JD087239 - Sequence 68263 from Patent EP1572962.
JD505831 - Sequence 486855 from Patent EP1572962.
JD371986 - Sequence 353010 from Patent EP1572962.
JD476956 - Sequence 457980 from Patent EP1572962.
JD476957 - Sequence 457981 from Patent EP1572962.
JD152118 - Sequence 133142 from Patent EP1572962.
JD322109 - Sequence 303133 from Patent EP1572962.
JD318202 - Sequence 299226 from Patent EP1572962.
JD203793 - Sequence 184817 from Patent EP1572962.
JD070104 - Sequence 51128 from Patent EP1572962.
JD070105 - Sequence 51129 from Patent EP1572962.
JD139547 - Sequence 120571 from Patent EP1572962.
JD120057 - Sequence 101081 from Patent EP1572962.
JD213264 - Sequence 194288 from Patent EP1572962.
JD542976 - Sequence 524000 from Patent EP1572962.
JD165281 - Sequence 146305 from Patent EP1572962.
JD262128 - Sequence 243152 from Patent EP1572962.
JD494593 - Sequence 475617 from Patent EP1572962.
BC098275 - Homo sapiens Nanog homeobox pseudogene 8, mRNA (cDNA clone IMAGE:40004923).
BC099704 - Homo sapiens Nanog homeobox pseudogene 8, mRNA (cDNA clone IMAGE:40004920).
JD085652 - Sequence 66676 from Patent EP1572962.
JD538990 - Sequence 520014 from Patent EP1572962.
JD402470 - Sequence 383494 from Patent EP1572962.
JD530845 - Sequence 511869 from Patent EP1572962.
JD254273 - Sequence 235297 from Patent EP1572962.
JD530844 - Sequence 511868 from Patent EP1572962.
JD526509 - Sequence 507533 from Patent EP1572962.
JD308569 - Sequence 289593 from Patent EP1572962.
HW243464 - JP 2013512690-A/15: RNA PREPARATIONS COMPRISING PURIFIED MODIFIED RNA FOR REPROGRAMMING CELLS.
LF715486 - JP 2016171804-A/15: RNA PREPARATIONS COMPRISING PURIFIED MODIFIED RNA FOR REPROGRAMMING CELLS.
EU446847 - Synthetic construct Homo sapiens clone IMAGE:100069947; IMAGE:100012056; FLH257151.01L Nanog homeobox (NANOG) gene, encodes complete protein.
KJ899593 - Synthetic construct Homo sapiens clone ccsbBroadEn_08987 NANOG gene, encodes complete protein.
JD526634 - Sequence 507658 from Patent EP1572962.
JD101724 - Sequence 82748 from Patent EP1572962.
JD500863 - Sequence 481887 from Patent EP1572962.
LY605669 - KR 1020180081836-A/15: RNA PREPARATIONS COMPRISING PURIFIED MODIFIED RNA FOR REPROGRAMMING CELLS.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000528386.1, ENST00000528386.2, ENST00000528386.3, J7H3Y9, J7H3Z5, J7H408, J7H412, J7H416, J7H738, J7H745, J7H749, J7H9S6, J7H9T4, J7HAW9, J7HAX1, J7HAX8, NANP8_HUMAN, NM_001355281, Q6NSW7, uc324epj.1, uc324epj.2
UCSC ID: ENST00000528386.4_10
RefSeq Accession: NM_001355281.2
Protein: Q6NSW7 (aka NANP8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.