Human Gene NANOS2 (ENST00000341294.4_6) from GENCODE V47lift37
  Description: nanos C2HC-type zinc finger 2 (from RefSeq NM_001029861.3)
Gencode Transcript: ENST00000341294.4_6
Gencode Gene: ENSG00000188425.4_8
Transcript (Including UTRs)
   Position: hg19 chr19:46,416,472-46,418,036 Size: 1,565 Total Exon Count: 1 Strand: -
Coding Region
   Position: hg19 chr19:46,417,535-46,417,951 Size: 417 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr19:46,416,472-46,418,036)mRNA (may differ from genome)Protein (138 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NANO2_HUMAN
DESCRIPTION: RecName: Full=Nanos homolog 2; Short=NOS-2;
FUNCTION: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state (By similarity).
SUBUNIT: Interacts with CNOT1, CNOT3, CNOT6L, CNOT7 and CNOT9 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm, P-body (By similarity). Cytoplasm, perinuclear region. Note=Localizes at P- bodies during gonocyte development (By similarity). More abundant in perinuclear region of the cytoplasm of the germ cells of the adult testis.
TISSUE SPECIFICITY: Testis and ovary. Expression found in several spermatogenic stages: in cells on the periphery of the tubules which could correspond to spermatogonia, in spermatocytes and in round spermatids (at protein level).
DEVELOPMENTAL STAGE: Fetal ovary and fetal testis. Present in all germ cells of seminiferous tubules of the 24-week fetus (at protein level).
DOMAIN: The Nanos-type zinc finger is composed of two C2HC motifs, each motif binding one molecule of zinc. It is essential for the translation repression activity of the protein (By similarity).
SIMILARITY: Belongs to the nanos family.
SIMILARITY: Contains 1 nanos-type zinc finger.

-  Primer design for this transcript
 

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Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • C547126 AZM551248
  • C045037 testosterone-3-carboxymethyloxime-bovine serum albumin conjugate

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 3.96 RPKM in Testis
Total median expression: 4.58 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -21.8085-0.256 Picture PostScript Text
3' UTR -378.301063-0.356 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008705 - Nanos/Xcar2
IPR024161 - Znf_nanos-typ

Pfam Domains:
PF05741 - Nanos RNA binding domain

SCOP Domains:
57783 - Zinc beta-ribbon
57802 - Rubredoxin-like
57938 - DnaJ/Hsp40 cysteine-rich domain

ModBase Predicted Comparative 3D Structure on P60321
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003729 mRNA binding
GO:0005515 protein binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding

Biological Process:
GO:0006402 mRNA catabolic process
GO:0006417 regulation of translation
GO:0007275 multicellular organism development
GO:0007283 spermatogenesis
GO:0017148 negative regulation of translation
GO:0030154 cell differentiation
GO:0030718 germ-line stem cell population maintenance
GO:0045835 negative regulation of meiotic nuclear division
GO:1900153 positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay

Cellular Component:
GO:0000932 P-body
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0048471 perinuclear region of cytoplasm


-  Descriptions from all associated GenBank mRNAs
  BC042883 - Homo sapiens nanos homolog 2 (Drosophila), mRNA (cDNA clone MGC:51340 IMAGE:5270224), complete cds.
JD077207 - Sequence 58231 from Patent EP1572962.
JD207328 - Sequence 188352 from Patent EP1572962.
JD447473 - Sequence 428497 from Patent EP1572962.
JD393576 - Sequence 374600 from Patent EP1572962.
JD495976 - Sequence 477000 from Patent EP1572962.
JD107747 - Sequence 88771 from Patent EP1572962.
JD325758 - Sequence 306782 from Patent EP1572962.
JD174327 - Sequence 155351 from Patent EP1572962.
JD086835 - Sequence 67859 from Patent EP1572962.
JD043174 - Sequence 24198 from Patent EP1572962.
JD361364 - Sequence 342388 from Patent EP1572962.
JD557513 - Sequence 538537 from Patent EP1572962.
JD564100 - Sequence 545124 from Patent EP1572962.
BC117484 - Homo sapiens nanos homolog 2 (Drosophila), mRNA (cDNA clone MGC:151093 IMAGE:40126035), complete cds.
BC117486 - Homo sapiens nanos homolog 2 (Drosophila), mRNA (cDNA clone MGC:151095 IMAGE:40126037), complete cds.
JD192931 - Sequence 173955 from Patent EP1572962.
JD136851 - Sequence 117875 from Patent EP1572962.
JD173143 - Sequence 154167 from Patent EP1572962.
JD434309 - Sequence 415333 from Patent EP1572962.
JD513275 - Sequence 494299 from Patent EP1572962.
HQ258065 - Synthetic construct Homo sapiens clone IMAGE:100072374 nanos homolog 2 (Drosophila) (NANOS2) gene, encodes complete protein.
KJ896064 - Synthetic construct Homo sapiens clone ccsbBroadEn_05458 NANOS2 gene, encodes complete protein.
JD386517 - Sequence 367541 from Patent EP1572962.
JD456703 - Sequence 437727 from Patent EP1572962.
JD403093 - Sequence 384117 from Patent EP1572962.
JD037172 - Sequence 18196 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000341294.1, ENST00000341294.2, ENST00000341294.3, NANO2_HUMAN, NM_001029861, NOS2, P60321, Q17R30, Q4G0P8, uc317vza.1, uc317vza.2
UCSC ID: ENST00000341294.4_6
RefSeq Accession: NM_001029861.3
Protein: P60321 (aka NANO2_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.