Human Gene NASP (ENST00000350030.8_5) from GENCODE V47lift37
  Description: nuclear autoantigenic sperm protein, transcript variant 2 (from RefSeq NM_002482.4)
Gencode Transcript: ENST00000350030.8_5
Gencode Gene: ENSG00000132780.17_16
Transcript (Including UTRs)
   Position: hg19 chr1:46,049,713-46,084,565 Size: 34,853 Total Exon Count: 15 Strand: +
Coding Region
   Position: hg19 chr1:46,049,819-46,083,813 Size: 33,995 Coding Exon Count: 15 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:46,049,713-46,084,565)mRNA (may differ from genome)Protein (788 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NASP_HUMAN
DESCRIPTION: RecName: Full=Nuclear autoantigenic sperm protein; Short=NASP;
FUNCTION: Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA (By similarity).
SUBUNIT: Binds to linker H1 histones but not to core histones. Also binds to HSP90 in the cytoplasm. This interaction stimulates binding of NASP to HIST1H1T/H1T (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus.
TISSUE SPECIFICITY: Isoform 1 is testis- and sperm-specific.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the NASP family.
SIMILARITY: Contains 3 TPR repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 47.17 RPKM in Testis
Total median expression: 881.37 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -30.80106-0.291 Picture PostScript Text
3' UTR -214.70752-0.286 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR019544 - Tetratricopeptide_SHNi-TPR_dom
IPR001440 - TPR-1
IPR013026 - TPR-contain_dom
IPR011990 - TPR-like_helical
IPR019734 - TPR_repeat

Pfam Domains:
PF10516 - SHNi-TPR
PF13181 - Tetratricopeptide repeat
PF13424 - Tetratricopeptide repeat

SCOP Domains:
81901 - HCP-like
48452 - TPR-like

ModBase Predicted Comparative 3D Structure on P49321
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0042393 histone binding
GO:0044877 macromolecular complex binding

Biological Process:
GO:0000082 G1/S transition of mitotic cell cycle
GO:0001824 blastocyst development
GO:0006260 DNA replication
GO:0006334 nucleosome assembly
GO:0006335 DNA replication-dependent nucleosome assembly
GO:0006336 DNA replication-independent nucleosome assembly
GO:0007049 cell cycle
GO:0008283 cell proliferation
GO:0008584 male gonad development
GO:0015031 protein transport
GO:0033574 response to testosterone
GO:0043486 histone exchange

Cellular Component:
GO:0000790 nuclear chromatin
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0032991 macromolecular complex


-  Descriptions from all associated GenBank mRNAs
  LF206563 - JP 2014500723-A/14066: Polycomb-Associated Non-Coding RNAs.
AK308001 - Homo sapiens cDNA, FLJ97949.
AK056161 - Homo sapiens cDNA FLJ31599 fis, clone NT2RI2002554, highly similar to NUCLEAR AUTOANTIGENIC SPERM PROTEIN.
AK222778 - Homo sapiens mRNA for nuclear autoantigenic sperm protein isoform 2 variant, clone: HEP00333.
AK222816 - Homo sapiens mRNA for nuclear autoantigenic sperm protein isoform 2 variant, clone: HEP06247.
AK298893 - Homo sapiens cDNA FLJ53158 complete cds, highly similar to Nuclear autoantigenic sperm protein.
AK291637 - Homo sapiens cDNA FLJ76872 complete cds, highly similar to Homo sapiens nuclear autoantigenic sperm protein (histone-binding) (NASP), transcript variant 2, mRNA.
AK299584 - Homo sapiens cDNA FLJ53176 complete cds, highly similar to Nuclear autoantigenic sperm protein.
BC010105 - Homo sapiens nuclear autoantigenic sperm protein (histone-binding), mRNA (cDNA clone MGC:19722 IMAGE:3538429), complete cds.
BC011580 - Homo sapiens nuclear autoantigenic sperm protein (histone-binding), mRNA (cDNA clone MGC:20372 IMAGE:4558913), complete cds.
AF035191 - Homo sapiens nuclear autoantigenic sperm protein autosomal variant mRNA, partial cds.
M97856 - Homo sapiens histone-binding protein mRNA, complete cds.
BC009933 - Homo sapiens nuclear autoantigenic sperm protein (histone-binding), mRNA (cDNA clone IMAGE:3959857), partial cds.
BC053608 - Homo sapiens nuclear autoantigenic sperm protein (histone-binding), mRNA (cDNA clone MGC:61522 IMAGE:6013263), complete cds.
JD180743 - Sequence 161767 from Patent EP1572962.
BC003113 - Homo sapiens nuclear autoantigenic sperm protein (histone-binding), mRNA (cDNA clone MGC:2297 IMAGE:3504220), complete cds.
JD305953 - Sequence 286977 from Patent EP1572962.
JD214403 - Sequence 195427 from Patent EP1572962.
DQ896606 - Synthetic construct Homo sapiens clone IMAGE:100011066; FLH195854.01L; RZPDo839H01151D nuclear autoantigenic sperm protein (histone-binding) (NASP) gene, encodes complete protein.
BT006757 - Homo sapiens nuclear autoantigenic sperm protein (histone-binding) mRNA, complete cds.
DQ893275 - Synthetic construct clone IMAGE:100005905; FLH195858.01X; RZPDo839H01152D nuclear autoantigenic sperm protein (histone-binding) (NASP) gene, encodes complete protein.
KJ897223 - Synthetic construct Homo sapiens clone ccsbBroadEn_06617 NASP gene, encodes complete protein.
LF356240 - JP 2014500723-A/163743: Polycomb-Associated Non-Coding RNAs.
AY700118 - Homo sapiens megakaryocytic differentiation related protein-like (HMDRA1) precursor RNA, complete sequence.
BC080632 - Homo sapiens cDNA clone IMAGE:6538748, **** WARNING: chimeric clone ****.
JD141763 - Sequence 122787 from Patent EP1572962.
JD178105 - Sequence 159129 from Patent EP1572962.
JD062313 - Sequence 43337 from Patent EP1572962.
AF113699 - Homo sapiens clone FLB7527 PRO1999 mRNA, complete cds.
JD108442 - Sequence 89466 from Patent EP1572962.
JD063583 - Sequence 44607 from Patent EP1572962.
AK131249 - Homo sapiens cDNA FLJ16175 fis, clone BRHIP2006617, highly similar to NUCLEAR AUTOANTIGENIC SPERM PROTEIN.
AK092829 - Homo sapiens cDNA FLJ35510 fis, clone SPLEN2000126, highly similar to NUCLEAR AUTOANTIGENIC SPERM PROTEIN.
JD339703 - Sequence 320727 from Patent EP1572962.
JD386191 - Sequence 367215 from Patent EP1572962.
JD348104 - Sequence 329128 from Patent EP1572962.
JD298730 - Sequence 279754 from Patent EP1572962.
JD508171 - Sequence 489195 from Patent EP1572962.
JD490485 - Sequence 471509 from Patent EP1572962.
AL512718 - Homo sapiens mRNA; cDNA DKFZp547F162 (from clone DKFZp547F162).
JD312455 - Sequence 293479 from Patent EP1572962.
JD497994 - Sequence 479018 from Patent EP1572962.
JD225592 - Sequence 206616 from Patent EP1572962.
JD453059 - Sequence 434083 from Patent EP1572962.
JD428729 - Sequence 409753 from Patent EP1572962.
JD360723 - Sequence 341747 from Patent EP1572962.
JD327688 - Sequence 308712 from Patent EP1572962.
JD291498 - Sequence 272522 from Patent EP1572962.
JD338360 - Sequence 319384 from Patent EP1572962.
JD188861 - Sequence 169885 from Patent EP1572962.
JD469945 - Sequence 450969 from Patent EP1572962.
JD279698 - Sequence 260722 from Patent EP1572962.
JD309807 - Sequence 290831 from Patent EP1572962.
MA442140 - JP 2018138019-A/14066: Polycomb-Associated Non-Coding RNAs.
MA591817 - JP 2018138019-A/163743: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K6H2, B4DQP3, D3DQ07, ENST00000350030.1, ENST00000350030.2, ENST00000350030.3, ENST00000350030.4, ENST00000350030.5, ENST00000350030.6, ENST00000350030.7, F5H3J2, NASP_HUMAN, NM_002482, P49321, Q53GW5, Q5T622, Q5T625, Q96A69, Q9BTW2, uc317xxw.1, uc317xxw.2
UCSC ID: ENST00000350030.8_5
RefSeq Accession: NM_002482.4
Protein: P49321 (aka NASP_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.