Human Gene NCKAP1L (ENST00000293373.11_6) from GENCODE V47lift37

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Description: NCK associated protein 1 like, transcript variant 1 (from RefSeq NM_005337.5)
Gencode Transcript: ENST00000293373.11_6
Gencode Gene: ENSG00000123338.13_8
Transcript (Including UTRs)
Position: hg19 chr12:54,891,536-54,942,027 Size: 50,492 Total Exon Count: 31 Strand: +
Coding Region
Position: hg19 chr12:54,891,574-54,936,469 Size: 44,896 Coding Exon Count: 31

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC: 2024-08-22 23:36:26

Sequence and Links to Tools and Databases

Genomic Sequence (chr12:54,891,536-54,942,027)			mRNA (may differ from genome)		Protein (1127 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
HGNC	MGI	OMIM	PubMed	Reactome	UniProtKB
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: NCKPL_HUMAN DESCRIPTION: RecName: Full=Nck-associated protein 1-like; AltName: Full=Hematopoietic protein 1; AltName: Full=Membrane-associated protein HEM-1; SUBCELLULAR LOCATION: Cell membrane; Single-pass membrane protein; Cytoplasmic side (Probable). TISSUE SPECIFICITY: Expressed only in cells of hematopoietic origin. SIMILARITY: Belongs to the HEM-1/HEM-2 family. SEQUENCE CAUTION: Sequence=AAA35964.1; Type=Erroneous initiation;

Primer design for this transcript

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D008767 Methylmercury Compounds D013749 Tetrachlorodibenzodioxin C016403 2,4-dinitrotoluene C547126 AZM551248 D000082 Acetaminophen D000535 Aluminum D001564 Benzo(a)pyrene D002794 Choline D004041 Dietary Fats D004997 Ethinyl Estradiol more ... click here to view the complete list

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 54.53 RPKM in Cells - EBV-transformed lymphocytes Total median expression: 261.29 RPKM View in GTEx track of Genome Browser View at GTEx portal View GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-6.10	38	-0.161	Picture	PostScript	Text
3' UTR	-1798.00	5558	-0.323	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR019137 - Nck-associated_protein-1

Pfam Domains:
PF09735 - Membrane-associated apoptosis protein

ModBase Predicted Comparative 3D Structure on P55160


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details	Gene Details
Gene Sorter	Gene Sorter
	RGD	Ensembl

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0005096 GTPase activator activity GO:0005515 protein binding GO:0030295 protein kinase activator activity GO:0044877 macromolecular complex binding Biological Process: GO:0001782 B cell homeostasis GO:0002262 myeloid cell homeostasis GO:0006935 chemotaxis GO:0030011 maintenance of cell polarity GO:0030593 neutrophil chemotaxis GO:0030838 positive regulation of actin filament polymerization GO:0030866 cortical actin cytoskeleton organization GO:0030890 positive regulation of B cell proliferation GO:0032147 activation of protein kinase activity GO:0032700 negative regulation of interleukin-17 production GO:0032715 negative regulation of interleukin-6 production GO:0033630 positive regulation of cell adhesion mediated by integrin GO:0034101 erythrocyte homeostasis GO:0035509 negative regulation of myosin-light-chain-phosphatase activity GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis GO:0042102 positive regulation of T cell proliferation GO:0042327 positive regulation of phosphorylation GO:0042493 response to drug GO:0043029 T cell homeostasis GO:0043066 negative regulation of apoptotic process GO:0043312 neutrophil degranulation GO:0043372 positive regulation of CD4-positive, alpha-beta T cell differentiation GO:0043378 positive regulation of CD8-positive, alpha-beta T cell differentiation GO:0043547 positive regulation of GTPase activity GO:0045579 positive regulation of B cell differentiation GO:0045588 positive regulation of gamma-delta T cell differentiation GO:0045621 positive regulation of lymphocyte differentiation GO:0045648 positive regulation of erythrocyte differentiation GO:0048010 vascular endothelial growth factor receptor signaling pathway GO:0048821 erythrocyte development GO:0050853 B cell receptor signaling pathway GO:0060100 positive regulation of phagocytosis, engulfment GO:0065003 macromolecular complex assembly GO:0070358 actin polymerization-dependent cell motility GO:0090023 positive regulation of neutrophil chemotaxis Cellular Component: GO:0005737 cytoplasm GO:0005829 cytosol GO:0005886 plasma membrane GO:0005887 integral component of plasma membrane GO:0016020 membrane GO:0016021 integral component of membrane GO:0030667 secretory granule membrane GO:0031209 SCAR complex GO:0070062 extracellular exosome GO:0101003 ficolin-1-rich granule membrane

Descriptions from all associated GenBank mRNAs


	M58285 - Human membrane-associated protein (HEM-1) mRNA, complete cds. BC093769 - Homo sapiens NCK-associated protein 1-like, mRNA (cDNA clone MGC:120804 IMAGE:7939614), complete cds. BC093771 - Homo sapiens NCK-associated protein 1-like, mRNA (cDNA clone MGC:120806 IMAGE:7939616), complete cds. BC143355 - Homo sapiens cDNA clone IMAGE:9051860, with apparent retained intron. AK300783 - Homo sapiens cDNA FLJ58731 complete cds, highly similar to Nck-associated protein 1-like. BC001604 - Homo sapiens NCK-associated protein 1-like, mRNA (cDNA clone IMAGE:3543376), partial cds. BC084547 - Homo sapiens NCK-associated protein 1-like, mRNA (cDNA clone IMAGE:5451042), partial cds. AK314012 - Homo sapiens cDNA, FLJ94667, highly similar to Homo sapiens hematopoietic protein 1 (HEM1), mRNA. BC064997 - Homo sapiens NCK-associated protein 1-like, mRNA (cDNA clone IMAGE:5786841), partial cds. JD103158 - Sequence 84182 from Patent EP1572962. JD271739 - Sequence 252763 from Patent EP1572962. JD310706 - Sequence 291730 from Patent EP1572962. JD507833 - Sequence 488857 from Patent EP1572962. JD250690 - Sequence 231714 from Patent EP1572962. JD524303 - Sequence 505327 from Patent EP1572962. JD039649 - Sequence 20673 from Patent EP1572962. JD455755 - Sequence 436779 from Patent EP1572962. JD363205 - Sequence 344229 from Patent EP1572962. JD286309 - Sequence 267333 from Patent EP1572962. JD315386 - Sequence 296410 from Patent EP1572962. JD269061 - Sequence 250085 from Patent EP1572962. JD111903 - Sequence 92927 from Patent EP1572962. JD530076 - Sequence 511100 from Patent EP1572962. JD102612 - Sequence 83636 from Patent EP1572962. JD167373 - Sequence 148397 from Patent EP1572962. JD230303 - Sequence 211327 from Patent EP1572962. JD183403 - Sequence 164427 from Patent EP1572962. JD548327 - Sequence 529351 from Patent EP1572962. JD282389 - Sequence 263413 from Patent EP1572962. JD410744 - Sequence 391768 from Patent EP1572962. JD166746 - Sequence 147770 from Patent EP1572962. JD063564 - Sequence 44588 from Patent EP1572962. JD435832 - Sequence 416856 from Patent EP1572962. JD132865 - Sequence 113889 from Patent EP1572962. JD542416 - Sequence 523440 from Patent EP1572962. JD133005 - Sequence 114029 from Patent EP1572962. JD455869 - Sequence 436893 from Patent EP1572962. JD289128 - Sequence 270152 from Patent EP1572962. JD077585 - Sequence 58609 from Patent EP1572962. JD485861 - Sequence 466885 from Patent EP1572962. JD486017 - Sequence 467041 from Patent EP1572962. JD492244 - Sequence 473268 from Patent EP1572962. JD101159 - Sequence 82183 from Patent EP1572962. JD339780 - Sequence 320804 from Patent EP1572962. JD475125 - Sequence 456149 from Patent EP1572962. JD137781 - Sequence 118805 from Patent EP1572962. JD196117 - Sequence 177141 from Patent EP1572962. JD222877 - Sequence 203901 from Patent EP1572962. JD142533 - Sequence 123557 from Patent EP1572962. JD222878 - Sequence 203902 from Patent EP1572962. JD211547 - Sequence 192571 from Patent EP1572962. JD497707 - Sequence 478731 from Patent EP1572962. JD497706 - Sequence 478730 from Patent EP1572962. JD147893 - Sequence 128917 from Patent EP1572962. JD239058 - Sequence 220082 from Patent EP1572962. JD542633 - Sequence 523657 from Patent EP1572962. JD542635 - Sequence 523659 from Patent EP1572962. JD542634 - Sequence 523658 from Patent EP1572962. JD019527 - Sequence 551 from Patent EP1572962. JD035716 - Sequence 16740 from Patent EP1572962.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein P55160 (Reactome details) participates in the following event(s): R-HSA-2029465 WAVE Regulatory Complex (WRC) interacts with RAC1 and PIP3 and gets activated R-HSA-6798743 Exocytosis of secretory granule membrane proteins R-HSA-6800426 Exocytosis of ficolin-rich granule membrane proteins R-HSA-2029469 p-ERK phosphorylates WAVEs and ABI R-HSA-2130194 ABL phosphorylates WAVEs R-HSA-442592 WASPs or WAVEs activate the ARP2/3 complex R-HSA-2197690 Detachment of WASP/WAVE R-HSA-2029466 Attachment of preexisting mother filament and initiation of branching R-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation R-HSA-4420097 VEGFA-VEGFR2 Pathway R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs R-HSA-6798695 Neutrophil degranulation R-HSA-2029480 Fcgamma receptor (FCGR) dependent phagocytosis R-HSA-194138 Signaling by VEGF R-HSA-195258 RHO GTPase Effectors R-HSA-168249 Innate Immune System R-HSA-9006934 Signaling by Receptor Tyrosine Kinases R-HSA-194315 Signaling by Rho GTPases R-HSA-168256 Immune System R-HSA-162582 Signal Transduction

Other Names for This Gene


	Alternate Gene Symbols: B4DUT5, ENST00000293373.1, ENST00000293373.10, ENST00000293373.2, ENST00000293373.3, ENST00000293373.4, ENST00000293373.5, ENST00000293373.6, ENST00000293373.7, ENST00000293373.8, ENST00000293373.9, HEM1 , NCKAP1L , NCKPL_HUMAN, NM_005337, P55160, Q52LW0, uc317ldn.1, uc317ldn.2 UCSC ID: ENST00000293373.11_6 RefSeq Accession: NM_005337.5 Protein: P55160 (aka NCKPL_HUMAN)

Gene Model Information


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Methods, Credits, and Use Restrictions


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