Human Gene NIFK (ENST00000285814.9_7) from GENCODE V47lift37
  Description: nucleolar protein interacting with the FHA domain of MKI67 (from RefSeq NM_032390.5)
Gencode Transcript: ENST00000285814.9_7
Gencode Gene: ENSG00000155438.12_10
Transcript (Including UTRs)
   Position: hg19 chr2:122,484,521-122,494,451 Size: 9,931 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr2:122,485,300-122,494,426 Size: 9,127 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersGene AllelesRNA-Seq Expression
Microarray ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:122,484,521-122,494,451)mRNA (may differ from genome)Protein (293 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
MGIOMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: MK67I_HUMAN
DESCRIPTION: RecName: Full=MKI67 FHA domain-interacting nucleolar phosphoprotein; AltName: Full=Nucleolar phosphoprotein Nopp34; AltName: Full=Nucleolar protein interacting with the FHA domain of pKI-67; Short=hNIFK;
SUBUNIT: Binds to the FHA domain of MKI67; this interaction is enhanced in mitosis.
SUBCELLULAR LOCATION: Nucleus, nucleolus. Chromosome. Note=Localizes to mitotic chromosomes in conjunction with MKI67.
PTM: Sequentially phosphorylated on Thr-238, Thr-234 and Ser-230. Thr-234 is phosphorylated only when Thr-238 is phosphorylated. Likewise, phosphorylation at Ser-230 requires that Thr-234 and Thr-238 are phosphorylated. Phosphorylation enhances MKI67 binding.
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 32.63 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 653.98 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -3.0025-0.120 Picture PostScript Text
3' UTR -179.10779-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR021043 - hNIFK_FHA_Ki67_binding
IPR012677 - Nucleotide-bd_a/b_plait
IPR000504 - RRM_dom

Pfam Domains:
PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
PF12196 - FHA Ki67 binding domain of hNIFK
PF13893 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

SCOP Domains:
54928 - RNA-binding domain, RBD

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2AFF - NMR MuPIT


ModBase Predicted Comparative 3D Structure on Q9BYG3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0005515 protein binding

Biological Process:
GO:0009303 rRNA transcription
GO:0016072 rRNA metabolic process
GO:0065003 macromolecular complex assembly

Cellular Component:
GO:0000794 condensed nuclear chromosome
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005694 chromosome
GO:0005730 nucleolus
GO:0005737 cytoplasm


-  Descriptions from all associated GenBank mRNAs
  BC024238 - Homo sapiens MKI67 (FHA domain) interacting nucleolar phosphoprotein, mRNA (cDNA clone MGC:32926 IMAGE:4797633), complete cds.
BC022990 - Homo sapiens MKI67 (FHA domain) interacting nucleolar phosphoprotein, mRNA (cDNA clone MGC:30198 IMAGE:4997441), complete cds.
JD281200 - Sequence 262224 from Patent EP1572962.
JD084977 - Sequence 66001 from Patent EP1572962.
AK291903 - Homo sapiens cDNA FLJ75749 complete cds, highly similar to Homo sapiens MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP), mRNA.
JD563449 - Sequence 544473 from Patent EP1572962.
JD044795 - Sequence 25819 from Patent EP1572962.
JD062648 - Sequence 43672 from Patent EP1572962.
JD066876 - Sequence 47900 from Patent EP1572962.
JD508981 - Sequence 490005 from Patent EP1572962.
JD288290 - Sequence 269314 from Patent EP1572962.
JD293812 - Sequence 274836 from Patent EP1572962.
JD376148 - Sequence 357172 from Patent EP1572962.
JD377162 - Sequence 358186 from Patent EP1572962.
JD543656 - Sequence 524680 from Patent EP1572962.
JD309781 - Sequence 290805 from Patent EP1572962.
LF349883 - JP 2014500723-A/157386: Polycomb-Associated Non-Coding RNAs.
JD316363 - Sequence 297387 from Patent EP1572962.
BC012457 - Homo sapiens, Similar to nucleolar protein interacting with the FHA domain of pKi-67, clone IMAGE:3882473, mRNA, partial cds.
AB044971 - Homo sapiens mRNA for nucleolar phosphoprotein Nopp34, complete cds.
KJ894993 - Synthetic construct Homo sapiens clone ccsbBroadEn_04387 MKI67IP gene, encodes complete protein.
KJ899791 - Synthetic construct Homo sapiens clone ccsbBroadEn_09185 MKI67IP gene, encodes complete protein.
DQ891678 - Synthetic construct clone IMAGE:100004308; FLH179209.01X; RZPDo839D12130D MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP) gene, encodes complete protein.
DQ894863 - Synthetic construct Homo sapiens clone IMAGE:100009323; FLH179205.01L; RZPDo839D12129D MKI67 (FHA domain) interacting nucleolar phosphoprotein (MKI67IP) gene, encodes complete protein.
AB528916 - Synthetic construct DNA, clone: pF1KE0703, Homo sapiens MKI67IP gene for MKI67 (FHA domain) interacting nucleolar phosphoprotein, without stop codon, in Flexi system.
CU692772 - Synthetic construct Homo sapiens gateway clone IMAGE:100016986 5' read MKI67IP mRNA.
LF349882 - JP 2014500723-A/157385: Polycomb-Associated Non-Coding RNAs.
AK311033 - Homo sapiens cDNA, FLJ18075.
AK299814 - Homo sapiens cDNA FLJ57495 complete cds, highly similar to MKI67 FHA domain-interacting nucleolar phosphoprotein.
LF349880 - JP 2014500723-A/157383: Polycomb-Associated Non-Coding RNAs.
LF349879 - JP 2014500723-A/157382: Polycomb-Associated Non-Coding RNAs.
AK027097 - Homo sapiens cDNA: FLJ23444 fis, clone HSI01343.
JD110802 - Sequence 91826 from Patent EP1572962.
MA585460 - JP 2018138019-A/157386: Polycomb-Associated Non-Coding RNAs.
MA585459 - JP 2018138019-A/157385: Polycomb-Associated Non-Coding RNAs.
MA585457 - JP 2018138019-A/157383: Polycomb-Associated Non-Coding RNAs.
MA585456 - JP 2018138019-A/157382: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K788, ENST00000285814.1, ENST00000285814.2, ENST00000285814.3, ENST00000285814.4, ENST00000285814.5, ENST00000285814.6, ENST00000285814.7, ENST00000285814.8, MK67I_HUMAN, MKI67IP, NM_032390, NOPP34, Q8TB66, Q96ED4, Q9BYG3, uc317kii.1, uc317kii.2
UCSC ID: ENST00000285814.9_7
RefSeq Accession: NM_032390.5
Protein: Q9BYG3 (aka MK67I_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.