ID:NIN_HUMAN DESCRIPTION: RecName: Full=Ninein; Short=hNinein; AltName: Full=Glycogen synthase kinase 3 beta-interacting protein; Short=GSK3B-interacting protein; FUNCTION: Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells. May also act as a centrosome maturation factor. May play a role in microtubule nucleation. Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules. SUBUNIT: Homooligomer. Interacts with GSK3B/GSK3-beta via its C- terminus domain. Interacts with C14ORF166, such interaction may prevent its phosphorylation by GSK3B. Interacts with AUNIP (via N- terminus). Identified in a complex with AUNIP and AURKA. INTERACTION: Q9Y224:C14orf166; NbExp=4; IntAct=EBI-1164022, EBI-1104547; P49841:GSK3B; NbExp=2; IntAct=EBI-1164022, EBI-373586; SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, centrosome. Note=Component of the core centrosome. Arranged in a tubular conformation with an open and a closed end within the centrosome. In the mother centrosome, it localizes at both ends of the centrosome tube, including the site of centrosome duplication, while in the daughter centrosome it is present only at the closed end. Requires PCM1 for centrosome localization. TISSUE SPECIFICITY: Ubiquitous. Highly expressed in heart and skeletal muscle. Isoform 1 is more expressed than isoform 5. DEVELOPMENTAL STAGE: In interphase cells, it is localized in the centrosome. Decreases in metaphase and anaphase and reappears in telophase. DOMAIN: The coiled coil region from Asn-1611 to Pro-1693 is necessary and sufficient for the targeting to centrosome. PTM: Phosphorylated by AURKA/Aurora kinase A and PKA kinases but not CK2 or AURKB/ Aurora kinase B. Phosphorylated upon DNA damage, probably by ATM or ATR. MISCELLANEOUS: Antibodies against NIN are present in sera from patients with autoimmune diseases that developed autoantibodies against centrosomal proteins. SIMILARITY: Contains 5 EF-hand domains. SEQUENCE CAUTION: Sequence=AAH65521.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=AAH65521.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence; Sequence=AAK00628.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors; Sequence=AAK00629.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors and frameshifts; Sequence=AAK00630.1; Type=Miscellaneous discrepancy; Note=Several sequencing errors; Sequence=BAB13391.2; Type=Erroneous initiation; Note=Translation N-terminally shortened; Sequence=BAB15640.1; Type=Erroneous initiation; Note=Translation N-terminally extended; WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/NINID176.html";
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q8N4C6
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
