Human Gene NMI (ENST00000243346.10_7) from GENCODE V47lift37
  Description: N-myc and STAT interactor (from RefSeq NM_004688.3)
Gencode Transcript: ENST00000243346.10_7
Gencode Gene: ENSG00000123609.11_11
Transcript (Including UTRs)
   Position: hg19 chr2:152,126,984-152,146,182 Size: 19,199 Total Exon Count: 8 Strand: -
Coding Region
   Position: hg19 chr2:152,127,207-152,139,462 Size: 12,256 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:152,126,984-152,146,182)mRNA (may differ from genome)Protein (307 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMGIOMIMPubMedUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NMI_HUMAN
DESCRIPTION: RecName: Full=N-myc-interactor; Short=Nmi; AltName: Full=N-myc and STAT interactor;
FUNCTION: May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5.
SUBUNIT: Interacts with MYCN and MYC, as well as with other transcription factors with a Zip, HLH or a HLH-Zip motif. Interacts with all STAT proteins except STAT2.
INTERACTION: Q9HCU9:BRMS1; NbExp=2; IntAct=EBI-372942, EBI-714781; Q9XRX5:HHLA3; NbExp=2; IntAct=EBI-372942, EBI-750554; P04198:MYCN; NbExp=3; IntAct=EBI-372942, EBI-878369; P56693:SOX10; NbExp=2; IntAct=EBI-372942, EBI-1167533; O55170:Sox10 (xeno); NbExp=4; IntAct=EBI-372942, EBI-1185693; P51692:STAT5B; NbExp=7; IntAct=EBI-372942, EBI-1186119; Q13748:TUBA3D; NbExp=2; IntAct=EBI-372942, EBI-355068;
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Expressed in all adult and fetal tissues except brain and skin. More abundant in fetal tissues especially liver.
INDUCTION: By IL2/interleukin-2 and IFNG/IFN-gamma.
SIMILARITY: Belongs to the NMI family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 32.93 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 308.84 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -32.7082-0.399 Picture PostScript Text
3' UTR -26.70223-0.120 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009938 - Interferon_induced_35kDa_N
IPR009909 - Nmi/IFP35
IPR012677 - Nucleotide-bd_a/b_plait

Pfam Domains:
PF07292 - Nmi/IFP 35 domain (NID)
PF07334 - Interferon-induced 35 kDa protein (IFP 35) N-terminus

SCOP Domains:
54928 - RNA-binding domain, RBD

ModBase Predicted Comparative 3D Structure on Q13287
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003712 transcription cofactor activity
GO:0005515 protein binding
GO:0042802 identical protein binding

Biological Process:
GO:0006366 transcription from RNA polymerase II promoter
GO:0006954 inflammatory response
GO:0007259 JAK-STAT cascade
GO:0032480 negative regulation of type I interferon production
GO:0045355 negative regulation of interferon-alpha biosynthetic process
GO:0045358 negative regulation of interferon-beta biosynthetic process
GO:0045824 negative regulation of innate immune response
GO:0060333 interferon-gamma-mediated signaling pathway
GO:1902524 positive regulation of protein K48-linked ubiquitination

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC001268 - Homo sapiens N-myc (and STAT) interactor, mRNA (cDNA clone MGC:5050 IMAGE:3452659), complete cds.
U32849 - Homo sapiens Nmi mRNA, complete cds.
AK291548 - Homo sapiens cDNA FLJ75708 complete cds, highly similar to Homo sapiens N-myc (and STAT) interactor (NMI), mRNA.
BC021987 - Homo sapiens N-myc (and STAT) interactor, mRNA (cDNA clone MGC:23722 IMAGE:4096531), complete cds.
AB451305 - Homo sapiens NMI mRNA for N-myc and STAT interactor, complete cds, clone: FLJ08162AAAN.
KJ897968 - Synthetic construct Homo sapiens clone ccsbBroadEn_07362 NMI gene, encodes complete protein.
AB451436 - Homo sapiens NMI mRNA for N-myc and STAT interactor, partial cds, clone: FLJ08162AAAF.
AB528048 - Synthetic construct DNA, clone: pF1KB7617, Homo sapiens NMI gene for N-myc (and STAT) interactor, without stop codon, in Flexi system.
CU692438 - Synthetic construct Homo sapiens gateway clone IMAGE:100017002 5' read NMI mRNA.
AK298754 - Homo sapiens cDNA FLJ58389 complete cds, highly similar to N-myc-interactor.
EF036506 - Homo sapiens N-myc-interactor mRNA, partial cds.
AK124323 - Homo sapiens cDNA FLJ42332 fis, clone TSTOM2001996, weakly similar to Homo sapiens Nmi mRNA.
JD170542 - Sequence 151566 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: B5BU69, ENST00000243346.1, ENST00000243346.2, ENST00000243346.3, ENST00000243346.4, ENST00000243346.5, ENST00000243346.6, ENST00000243346.7, ENST00000243346.8, ENST00000243346.9, NMI , NMI_HUMAN, NM_004688, Q13287, Q53TI8, Q8WTW2, Q9BVE5, uc317emd.1, uc317emd.2
UCSC ID: ENST00000243346.10_7
RefSeq Accession: NM_004688.3
Protein: Q13287 (aka NMI_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.