Human Gene NOB1 (ENST00000268802.10_7) from GENCODE V47lift37
  Description: NIN1 (RPN12) binding protein 1 homolog, transcript variant 2 (from RefSeq NR_074074.2)
Gencode Transcript: ENST00000268802.10_7
Gencode Gene: ENSG00000141101.13_9
Transcript (Including UTRs)
   Position: hg19 chr16:69,775,774-69,788,829 Size: 13,056 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg19 chr16:69,776,235-69,788,813 Size: 12,579 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:69,775,774-69,788,829)mRNA (may differ from genome)Protein (412 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NOB1_HUMAN
DESCRIPTION: RecName: Full=RNA-binding protein NOB1; AltName: Full=Phosphorylation regulatory protein HP-10; AltName: Full=Protein ART-4;
FUNCTION: May play a role in mRNA degradation.
SUBUNIT: Interacts with UPF2 (Probable).
INTERACTION: P04156:PRNP; NbExp=1; IntAct=EBI-373285, EBI-977302; Q9HAU5:UPF2; NbExp=1; IntAct=EBI-373285, EBI-372073;
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Detected in liver, lung, placenta, endothelial cells and spleen.
SIMILARITY: Belongs to the NOB1 family.
SIMILARITY: Contains 1 PINc domain.
SEQUENCE CAUTION: Sequence=AAQ13705.1; Type=Frameshift; Positions=393; Sequence=BAA91473.1; Type=Frameshift; Positions=87;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NOB1
Diseases sorted by gene-association score: deafness, autosomal recessive 89 (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 63.60 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 1085.55 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR 0.00160.000 Picture PostScript Text
3' UTR -142.30461-0.309 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR017117 - D-site_20S_pre-rRNA_nuclease
IPR014881 - NOB1_Zn-bd
IPR006596 - PINc_nuc-bd

Pfam Domains:
PF08772 - Nin one binding (NOB1) Zn-ribbon like
PF15017 - Putative WW-binding domain and destruction box
PF17146 - PIN domain of ribonuclease

SCOP Domains:
144206 - NOB1 zinc finger-like

ModBase Predicted Comparative 3D Structure on Q9ULX3
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
Gene SorterGene Sorter Gene SorterGene SorterGene Sorter
 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004521 endoribonuclease activity
GO:0046872 metal ion binding

Biological Process:
GO:0000469 cleavage involved in rRNA processing
GO:0006364 rRNA processing
GO:0007601 visual perception
GO:0030490 maturation of SSU-rRNA
GO:0042274 ribosomal small subunit biogenesis
GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0030688 preribosome, small subunit precursor


-  Descriptions from all associated GenBank mRNAs
  AK126196 - Homo sapiens cDNA FLJ44208 fis, clone THYMU3001991, moderately similar to Homo sapiens ART-4 protein (ART-4).
AF190161 - Homo sapiens MSTP158 (MST158) mRNA, complete cds.
AB026125 - Homo sapiens ART-4 mRNA, complete cds.
AY487344 - Homo sapiens RNA-binding protein NOB1 mRNA, complete cds.
AK001028 - Homo sapiens cDNA FLJ10166 fis, clone HEMBA1003615.
BC064630 - Homo sapiens NIN1/RPN12 binding protein 1 homolog (S. cerevisiae), mRNA (cDNA clone MGC:75033 IMAGE:5740890), complete cds.
BC000050 - Homo sapiens NIN1/RPN12 binding protein 1 homolog (S. cerevisiae), mRNA (cDNA clone IMAGE:3504549), partial cds.
JD342769 - Sequence 323793 from Patent EP1572962.
JD439510 - Sequence 420534 from Patent EP1572962.
AK314307 - Homo sapiens cDNA, FLJ95064, highly similar to Homo sapiens nin one binding protein (NOB1P), mRNA.
KJ893663 - Synthetic construct Homo sapiens clone ccsbBroadEn_03057 NOB1 gene, encodes complete protein.
CU687926 - Synthetic construct Homo sapiens gateway clone IMAGE:100021799 5' read NOB1 mRNA.
JD040553 - Sequence 21577 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9ULX3 (Reactome details) participates in the following event(s):

R-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol
R-HSA-8868773 rRNA processing in the nucleus and cytosol
R-HSA-72312 rRNA processing
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: ART4, ENST00000268802.1, ENST00000268802.2, ENST00000268802.3, ENST00000268802.4, ENST00000268802.5, ENST00000268802.6, ENST00000268802.7, ENST00000268802.8, ENST00000268802.9, MSTP158, NOB1P, NOB1_HUMAN, NR_074074, PSMD8BP1, Q7L6B7, Q7M4M4, Q7Z4B5, Q9NWB0, Q9ULX3, uc317ioy.1, uc317ioy.2
UCSC ID: ENST00000268802.10_7
RefSeq Accession: NM_014062.3
Protein: Q9ULX3 (aka NOB1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.