Human Gene NOX1 (ENST00000372966.8_7) from GENCODE V47lift37
  Description: NADPH oxidase 1, transcript variant 1 (from RefSeq NM_007052.5)
Gencode Transcript: ENST00000372966.8_7
Gencode Gene: ENSG00000007952.18_14
Transcript (Including UTRs)
   Position: hg19 chrX:100,098,313-100,129,348 Size: 31,036 Total Exon Count: 13 Strand: -
Coding Region
   Position: hg19 chrX:100,098,941-100,129,128 Size: 30,188 Coding Exon Count: 13 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrX:100,098,313-100,129,348)mRNA (may differ from genome)Protein (564 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NOX1_HUMAN
DESCRIPTION: RecName: Full=NADPH oxidase 1; Short=NOX-1; EC=1.-.-.-; AltName: Full=Mitogenic oxidase 1; Short=MOX-1; AltName: Full=NADH/NADPH mitogenic oxidase subunit P65-MOX; AltName: Full=NOH-1;
FUNCTION: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
COFACTOR: NADP (Potential).
COFACTOR: FAD (Potential).
ENZYME REGULATION: The oxidase activity is potentiated by NOXA1 and NOXO1.
SUBUNIT: NOX1, NOXA1, NOXO1, RAC1 and CYBA forms a functional multimeric complex supporting ROS production. Interacts with NOXA1 and NOXO1.
SUBCELLULAR LOCATION: Cell projection, invadopodium membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH- 1S is detected only in colon and colon carcinoma cells.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 ferric oxidoreductase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NOX1
Diseases sorted by gene-association score: retinitis pigmentosa 47 (7), chronic granulomatous disease (6), synucleinopathy (6), galactosemia (5), vascular disease (3)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 18.72 RPKM in Colon - Transverse
Total median expression: 24.79 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -61.60220-0.280 Picture PostScript Text
3' UTR -139.10628-0.221 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000778 - Cyt_b245_heavy_chain
IPR013112 - FAD-bd_8
IPR017927 - Fd_Rdtase_FAD-bd
IPR013130 - Fe3_Rdtase_TM_dom
IPR013121 - Fe_red_NAD-bd_6
IPR017938 - Riboflavin_synthase-like_b-brl

Pfam Domains:
PF01794 - Ferric reductase like transmembrane component
PF08022 - FAD-binding domain
PF08030 - Ferric reductase NAD binding domain

SCOP Domains:
63380 - Riboflavin synthase domain-like
52343 - Ferredoxin reductase-like, C-terminal NADP-linked domain

ModBase Predicted Comparative 3D Structure on Q9Y5S8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005244 voltage-gated ion channel activity
GO:0005515 protein binding
GO:0016175 superoxide-generating NADPH oxidase activity
GO:0016491 oxidoreductase activity
GO:0046872 metal ion binding
GO:0048365 Rac GTPase binding
GO:0050661 NADP binding

Biological Process:
GO:0001525 angiogenesis
GO:0003081 regulation of systemic arterial blood pressure by renin-angiotensin
GO:0006739 NADP metabolic process
GO:0006811 ion transport
GO:0006954 inflammatory response
GO:0007165 signal transduction
GO:0008217 regulation of blood pressure
GO:0008284 positive regulation of cell proliferation
GO:0010575 positive regulation of vascular endothelial growth factor production
GO:0016477 cell migration
GO:0030198 extracellular matrix organization
GO:0034220 ion transmembrane transport
GO:0034765 regulation of ion transmembrane transport
GO:0042554 superoxide anion generation
GO:0042743 hydrogen peroxide metabolic process
GO:0043410 positive regulation of MAPK cascade
GO:0045726 positive regulation of integrin biosynthetic process
GO:0045730 respiratory burst
GO:0046330 positive regulation of JNK cascade
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0051454 intracellular pH elevation
GO:0055114 oxidation-reduction process
GO:0071455 cellular response to hyperoxia
GO:0072592 oxygen metabolic process
GO:1902177 positive regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
GO:1990451 cellular stress response to acidic pH

Cellular Component:
GO:0005737 cytoplasm
GO:0005768 endosome
GO:0005769 early endosome
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030054 cell junction
GO:0042995 cell projection
GO:0043020 NADPH oxidase complex
GO:0071438 invadopodium membrane


-  Descriptions from all associated GenBank mRNAs
  LF211651 - JP 2014500723-A/19154: Polycomb-Associated Non-Coding RNAs.
AF127763 - Homo sapiens mitogenic oxidase mRNA, complete cds.
HW061176 - JP 2012529430-A/51: METHODS FOR TREATING CHRONIC KIDNEY DISEASE.
HW061177 - JP 2012529430-A/52: METHODS FOR TREATING CHRONIC KIDNEY DISEASE.
JA482136 - Sequence 119 from Patent WO2011072091.
JA482137 - Sequence 120 from Patent WO2011072091.
JB251984 - Sequence 51 from Patent EP2440214.
JB251985 - Sequence 52 from Patent EP2440214.
JE980428 - Sequence 119 from Patent EP2862929.
JE980429 - Sequence 120 from Patent EP2862929.
LP764883 - Sequence 51 from Patent EP3276004.
LP764884 - Sequence 52 from Patent EP3276004.
AF166326 - Homo sapiens NADPH oxidase homolog 1 short form (NOH1) mRNA, alternatively spliced, complete cds.
AK292201 - Homo sapiens cDNA FLJ77172 complete cds, highly similar to Homo sapiens NADPH oxidase 1, transcript variant NOH-1L, mRNA.
JD489352 - Sequence 470376 from Patent EP1572962.
JD045248 - Sequence 26272 from Patent EP1572962.
JD456064 - Sequence 437088 from Patent EP1572962.
JD305094 - Sequence 286118 from Patent EP1572962.
JD378607 - Sequence 359631 from Patent EP1572962.
LF383580 - JP 2014500723-A/191083: Polycomb-Associated Non-Coding RNAs.
JD181799 - Sequence 162823 from Patent EP1572962.
AF166327 - Homo sapiens NADPH oxidase homolog 1 long form (NOH1) mRNA, alternatively spliced, complete cds.
AF166328 - Homo sapiens NADPH oxidase homolog 1 long form variant (NOH1) mRNA, alternatively spliced, complete cds.
BC075014 - Homo sapiens NADPH oxidase 1, mRNA (cDNA clone MGC:104158 IMAGE:30915618), complete cds.
BC075015 - Homo sapiens NADPH oxidase 1, transcript variant NOH-1L, mRNA (cDNA clone MGC:103997 IMAGE:30915405), complete cds.
AJ438989 - Homo sapiens mRNA for nox1 NAD(P)H-oxidase subunit (NOX1 gene).
DQ424904 - Homo sapiens clone FG060307 NADPH oxidase 1 variant NOH-1L mRNA, complete cds, alternatively spliced.
AK313678 - Homo sapiens cDNA, FLJ94265.
KJ898651 - Synthetic construct Homo sapiens clone ccsbBroadEn_08045 NOX1 gene, encodes complete protein.
JD272260 - Sequence 253284 from Patent EP1572962.
JD171592 - Sequence 152616 from Patent EP1572962.
JD299769 - Sequence 280793 from Patent EP1572962.
JD244618 - Sequence 225642 from Patent EP1572962.
JD182084 - Sequence 163108 from Patent EP1572962.
MA619157 - JP 2018138019-A/191083: Polycomb-Associated Non-Coding RNAs.
MA447228 - JP 2018138019-A/19154: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_CCR3Pathway - CCR3 signaling in Eosinophils
h_freePathway - Free Radical Induced Apoptosis

Reactome (by CSHL, EBI, and GO)

Protein Q9Y5S8 (Reactome details) participates in the following event(s):

R-HSA-5668714 RAC1:GTP binds NOX1 complex
R-HSA-5668718 NOX1 complex:RAC1:GTP generates superoxide from oxygen
R-HSA-5668599 RHO GTPases Activate NADPH Oxidases
R-HSA-195258 RHO GTPase Effectors
R-HSA-194315 Signaling by Rho GTPases
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: A8K836, ENST00000372966.1, ENST00000372966.2, ENST00000372966.3, ENST00000372966.4, ENST00000372966.5, ENST00000372966.6, ENST00000372966.7, MOX1, NM_007052, NOH1, NOX1_HUMAN, O95691, Q2PP02, Q9Y5S8, uc318kjc.1, uc318kjc.2
UCSC ID: ENST00000372966.8_7
RefSeq Accession: NM_007052.5
Protein: Q9Y5S8 (aka NOX1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.