ID:NOX5_HUMAN DESCRIPTION: RecName: Full=NADPH oxidase 5; EC=1.6.3.-; FUNCTION: Calcium-dependent NADPH oxidase that generates superoxide. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. May play a role in cell growth and apoptosis. Isoform v2 and isoform v5 are involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. COFACTOR: FAD. COFACTOR: Magnesium. ENZYME REGULATION: Activated by calcium which induces conformational changes and interaction between the N-terminal regulatory region and the C-terminal catalytic region. Inhibited by diphenylene iodonium. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=1.06 uM for calcium; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Potential). SUBCELLULAR LOCATION: Isoform v2: Endoplasmic reticulum. SUBCELLULAR LOCATION: Isoform v5: Endoplasmic reticulum. TISSUE SPECIFICITY: Mainly expressed in pachytene spermatocytes of testis and in lymphocyte-rich areas of spleen and lymph nodes. Isoform v1 is expressed in spleen. Isoform v2 is expressed in testis. Also detected in ovary, placenta, pancreas, cardiac fibroblasts. Expressed in B-cells and prostate malignant cells. Isoform v1 and isoform v3 are expressed in epithelial colorectal adenocarcinoma cells. Isoform v2 and isoform v4 are expressed in endothelial cells. Isoform v1, isoform v2, isoform v3 and isoform v4 are expressed in pulmonary artery smooth muscle cells. Isoform v2 and isoform v5 are expressed in microvascular endothelial cells (at protein level). DEVELOPMENTAL STAGE: Expressed in fetal tissues. INDUCTION: Down-regulated by TGFB1. DOMAIN: Isoform v1 and isoform v2 of this protein have four functional EF-hand calcium-binding domains. Isoform v3 and isoform v4 have the third EF-hand domain interrupted by an insert. SIMILARITY: Contains 4 EF-hand domains. SIMILARITY: Contains 1 FAD-binding FR-type domain. SIMILARITY: Contains 1 ferric oxidoreductase domain. SEQUENCE CAUTION: Sequence=AAG33638.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAB15319.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAB84897.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q96PH1
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Mouse
Rat
Zebrafish
D. melanogaster
C. elegans
S. cerevisiae
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
No ortholog
Gene Ontology (GO) Annotations with Structured Vocabulary
Biological Process: GO:0001525 angiogenesis GO:0001935 endothelial cell proliferation GO:0006811 ion transport GO:0006915 apoptotic process GO:0008283 cell proliferation GO:0010155 regulation of proton transport GO:0034599 cellular response to oxidative stress GO:0042554 superoxide anion generation GO:0043012 regulation of fusion of sperm to egg plasma membrane GO:0050663 cytokine secretion GO:0055114 oxidation-reduction process GO:0061640 cytoskeleton-dependent cytokinesis GO:1902600 hydrogen ion transmembrane transport GO:2000379 positive regulation of reactive oxygen species metabolic process
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
