Human Gene NQO1 (ENST00000320623.10_7) from GENCODE V47lift37
  Description: NAD(P)H quinone dehydrogenase 1, transcript variant 1 (from RefSeq NM_000903.3)
Gencode Transcript: ENST00000320623.10_7
Gencode Gene: ENSG00000181019.13_9
Transcript (Including UTRs)
   Position: hg19 chr16:69,743,304-69,760,463 Size: 17,160 Total Exon Count: 6 Strand: -
Coding Region
   Position: hg19 chr16:69,744,879-69,760,342 Size: 15,464 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:69,743,304-69,760,463)mRNA (may differ from genome)Protein (274 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: NQO1_HUMAN
DESCRIPTION: RecName: Full=NAD(P)H dehydrogenase [quinone] 1; EC=1.6.5.2; AltName: Full=Azoreductase; AltName: Full=DT-diaphorase; Short=DTD; AltName: Full=Menadione reductase; AltName: Full=NAD(P)H:quinone oxidoreductase 1; AltName: Full=Phylloquinone reductase; AltName: Full=Quinone reductase 1; Short=QR1;
FUNCTION: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
CATALYTIC ACTIVITY: NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone.
COFACTOR: FAD.
ENZYME REGULATION: Inhibited by dicoumarol.
SUBUNIT: Homodimer.
INTERACTION: O88898-2:Tp63 (xeno); NbExp=2; IntAct=EBI-3989435, EBI-2338228; O88898-6:Tp63 (xeno); NbExp=2; IntAct=EBI-3989435, EBI-2338244;
SUBCELLULAR LOCATION: Cytoplasm.
INDUCTION: By dioxin.
MASS SPECTROMETRY: Mass=30864; Mass_error=6; Method=Electrospray; Range=1-274; Source=PubMed:11735396;
POLYMORPHISM: The Ser-187 polymorphism may be linked to susceptibility to forms of cancers.
MISCELLANEOUS: Quinone reductase accepts electrons from both NADH and NADPH with equal efficiency.
SIMILARITY: Belongs to the NAD(P)H dehydrogenase (quinone) family.
WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and Haematology; URL="http://atlasgeneticsoncology.org/Genes/NQO1ID375.html";
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/nqo1/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: NQO1
Diseases sorted by gene-association score: tardive dyskinesia (29), north american indian childhood cirrhosis (13), lung cancer (11), atrophy of testis (9), anal canal carcinoma (8), colon adenocarcinoma (8), childhood leukemia (6), acute lymphoblastic leukemia, childhood (6), breast adenocarcinoma (5), colorectal cancer (4), alzheimer disease (2), myelodysplastic syndrome (2), parkinson disease, late-onset (1)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 80.97 RPKM in Cells - Cultured fibroblasts
Total median expression: 661.14 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -36.70121-0.303 Picture PostScript Text
3' UTR -407.701575-0.259 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR003680 - Flavodoxin_fold

Pfam Domains:
PF02525 - Flavodoxin-like fold
PF03358 - NADPH-dependent FMN reductase

SCOP Domains:
52218 - Flavoproteins

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1D4A - X-ray MuPIT 1DXO - X-ray MuPIT 1GG5 - X-ray MuPIT 1H66 - X-ray MuPIT 1H69 - X-ray MuPIT 1KBO - X-ray MuPIT 1KBQ - X-ray MuPIT 1QBG - X-ray MuPIT 2F1O - X-ray MuPIT 3JSX - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P15559
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003955 NAD(P)H dehydrogenase (quinone) activity
GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
GO:0004784 superoxide dismutase activity
GO:0005515 protein binding
GO:0016491 oxidoreductase activity
GO:0042802 identical protein binding

Biological Process:
GO:0006521 regulation of cellular amino acid metabolic process
GO:0006801 superoxide metabolic process
GO:0006805 xenobiotic metabolic process
GO:0006809 nitric oxide biosynthetic process
GO:0006979 response to oxidative stress
GO:0007271 synaptic transmission, cholinergic
GO:0007568 aging
GO:0007584 response to nutrient
GO:0009636 response to toxic substance
GO:0014070 response to organic cyclic compound
GO:0019430 removal of superoxide radicals
GO:0032355 response to estradiol
GO:0043066 negative regulation of apoptotic process
GO:0043086 negative regulation of catalytic activity
GO:0043525 positive regulation of neuron apoptotic process
GO:0045471 response to ethanol
GO:0051602 response to electrical stimulus
GO:0055114 oxidation-reduction process
GO:0070301 cellular response to hydrogen peroxide
GO:0071248 cellular response to metal ion
GO:1901698 response to nitrogen compound
GO:1904880 response to hydrogen sulfide

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0030425 dendrite
GO:0043025 neuronal cell body


-  Descriptions from all associated GenBank mRNAs
  LF384450 - JP 2014500723-A/191953: Polycomb-Associated Non-Coding RNAs.
LP747429 - Sequence 16 from Patent WO2018009939.
J03934 - Human, NAD(P)H:menadione oxidoreductase mRNA, complete cds.
JD037774 - Sequence 18798 from Patent EP1572962.
JD079270 - Sequence 60294 from Patent EP1572962.
JD137447 - Sequence 118471 from Patent EP1572962.
JD346546 - Sequence 327570 from Patent EP1572962.
JD312052 - Sequence 293076 from Patent EP1572962.
JD330984 - Sequence 312008 from Patent EP1572962.
JD242701 - Sequence 223725 from Patent EP1572962.
JD329755 - Sequence 310779 from Patent EP1572962.
JD329754 - Sequence 310778 from Patent EP1572962.
JD441688 - Sequence 422712 from Patent EP1572962.
JD111224 - Sequence 92248 from Patent EP1572962.
JD111225 - Sequence 92249 from Patent EP1572962.
JD385843 - Sequence 366867 from Patent EP1572962.
JD455935 - Sequence 436959 from Patent EP1572962.
JD455936 - Sequence 436960 from Patent EP1572962.
JD384820 - Sequence 365844 from Patent EP1572962.
JD564130 - Sequence 545154 from Patent EP1572962.
LF374451 - JP 2014500723-A/181954: Polycomb-Associated Non-Coding RNAs.
JD067455 - Sequence 48479 from Patent EP1572962.
JD450717 - Sequence 431741 from Patent EP1572962.
LF374452 - JP 2014500723-A/181955: Polycomb-Associated Non-Coding RNAs.
JD059160 - Sequence 40184 from Patent EP1572962.
JD092406 - Sequence 73430 from Patent EP1572962.
JD315245 - Sequence 296269 from Patent EP1572962.
LF374453 - JP 2014500723-A/181956: Polycomb-Associated Non-Coding RNAs.
AK316246 - Homo sapiens cDNA, FLJ79145 complete cds, highly similar to Homo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1).
JD506754 - Sequence 487778 from Patent EP1572962.
JD506821 - Sequence 487845 from Patent EP1572962.
AK297979 - Homo sapiens cDNA FLJ50591 complete cds, highly similar to Homo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 3, mRNA.
JD209285 - Sequence 190309 from Patent EP1572962.
JD217923 - Sequence 198947 from Patent EP1572962.
AK298896 - Homo sapiens cDNA FLJ60804 complete cds, highly similar to NAD(P)H dehydrogenase (quinone) 1 (EC 1.6.5.2).
LF374454 - JP 2014500723-A/181957: Polycomb-Associated Non-Coding RNAs.
AK297125 - Homo sapiens cDNA FLJ50573 complete cds, highly similar to Homo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1), transcript variant 3, mRNA.
AK309513 - Homo sapiens cDNA, FLJ99554.
AK223050 - Homo sapiens mRNA for NAD(P)H menadione oxidoreductase 1, dioxin-inducible variant, clone: KAT00116.
BC007659 - Homo sapiens NAD(P)H dehydrogenase, quinone 1, mRNA (cDNA clone MGC:2073 IMAGE:3349257), complete cds.
BC000906 - Homo sapiens NAD(P)H dehydrogenase, quinone 1, mRNA (cDNA clone IMAGE:2901105), containing frame-shift errors.
AK307533 - Homo sapiens cDNA, FLJ97481.
JD095030 - Sequence 76054 from Patent EP1572962.
JD049343 - Sequence 30367 from Patent EP1572962.
AK312368 - Homo sapiens cDNA, FLJ92690, Homo sapiens NAD(P)H dehydrogenase, quinone 1 (NQO1), mRNA.
DQ892892 - Synthetic construct clone IMAGE:100005522; FLH190704.01X; RZPDo839D0276D NAD(P)H dehydrogenase, quinone 1 (NQO1) gene, encodes complete protein.
DQ896139 - Synthetic construct Homo sapiens clone IMAGE:100010599; FLH190700.01L; RZPDo839D0266D NAD(P)H dehydrogenase, quinone 1 (NQO1) gene, encodes complete protein.
AB590179 - Synthetic construct DNA, clone: pFN21AE1444, Homo sapiens NQO1 gene for NAD(P)H dehydrogenase, quinone 1, without stop codon, in Flexi system.
LF374455 - JP 2014500723-A/181958: Polycomb-Associated Non-Coding RNAs.
BC107739 - Homo sapiens NAD(P)H dehydrogenase, quinone 1, mRNA (cDNA clone IMAGE:6463260), partial cds.
LF374456 - JP 2014500723-A/181959: Polycomb-Associated Non-Coding RNAs.
LQ021082 - Sequence 25 from Patent WO2015144924.
JD022968 - Sequence 3992 from Patent EP1572962.
JD036027 - Sequence 17051 from Patent EP1572962.
JD020329 - Sequence 1353 from Patent EP1572962.
JD034993 - Sequence 16017 from Patent EP1572962.
JD021157 - Sequence 2181 from Patent EP1572962.
MA620027 - JP 2018138019-A/191953: Polycomb-Associated Non-Coding RNAs.
MA610028 - JP 2018138019-A/181954: Polycomb-Associated Non-Coding RNAs.
MA610029 - JP 2018138019-A/181955: Polycomb-Associated Non-Coding RNAs.
MA610030 - JP 2018138019-A/181956: Polycomb-Associated Non-Coding RNAs.
MA610031 - JP 2018138019-A/181957: Polycomb-Associated Non-Coding RNAs.
MA610032 - JP 2018138019-A/181958: Polycomb-Associated Non-Coding RNAs.
MA610033 - JP 2018138019-A/181959: Polycomb-Associated Non-Coding RNAs.
MB486569 - JP 2019531699-A/16: METHODS FOR DIAGNOSING AND TREATING CANCER.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7999 - vitamin K-epoxide cycle

BioCarta from NCI Cancer Genome Anatomy Project
h_p53hypoxiaPathway - Hypoxia and p53 in the Cardiovascular system

Reactome (by CSHL, EBI, and GO)

Protein P15559 (Reactome details) participates in the following event(s):

R-HSA-350578 NQO1 interaction with ODC
R-HSA-350562 Regulation of ornithine decarboxylase (ODC)
R-HSA-351202 Metabolism of polyamines
R-HSA-71291 Metabolism of nitrogenous molecules
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B2R5Y9, B4DNM7, B7ZAD1, DIA4, ENST00000320623.1, ENST00000320623.2, ENST00000320623.3, ENST00000320623.4, ENST00000320623.5, ENST00000320623.6, ENST00000320623.7, ENST00000320623.8, ENST00000320623.9, NMOR1, NM_000903, NQO1 , NQO1_HUMAN, P15559, Q86UK1, uc317qxh.1, uc317qxh.2
UCSC ID: ENST00000320623.10_7
RefSeq Accession: NM_000903.3
Protein: P15559 (aka NQO1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.