ID:NSF_HUMAN DESCRIPTION: RecName: Full=Vesicle-fusing ATPase; EC=3.6.4.6; AltName: Full=N-ethylmaleimide-sensitive fusion protein; Short=NEM-sensitive fusion protein; AltName: Full=Vesicular-fusion protein NSF; FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seem to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity). CATALYTIC ACTIVITY: ATP + H(2)O = ADP + phosphate. COFACTOR: Binds 1 magnesium ion per subunit (By similarity). SUBUNIT: Homohexamer. Interacts with GABARAP and GABARAPL2. Interacts with GRIA2. Interacts with PLK2, leading to disrupt the interaction with GRIA2. Interacts with MUSK; may regulate MUSK endocytosis and activity (By similarity). Interacts with CDK16 (By similarity). SUBCELLULAR LOCATION: Cytoplasm. PTM: Phosphorylation at Ser-569 interferes with homohexamerization (By similarity). SIMILARITY: Belongs to the AAA ATPase family. SEQUENCE CAUTION: Sequence=AAA17411.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P46459
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001921 positive regulation of receptor recycling GO:0006813 potassium ion transport GO:0006886 intracellular protein transport GO:0006887 exocytosis GO:0006888 ER to Golgi vesicle-mediated transport GO:0006890 retrograde vesicle-mediated transport, Golgi to ER GO:0015031 protein transport GO:0016192 vesicle-mediated transport GO:0017157 regulation of exocytosis GO:0035494 SNARE complex disassembly GO:0045026 plasma membrane fusion GO:0045732 positive regulation of protein catabolic process GO:0048208 COPII vesicle coating
AF135168 - Homo sapiens N-ethylmaleimide-sensitive factor (NSF) mRNA, complete cds. LP895342 - Sequence 206 from Patent EP3253886. AK294727 - Homo sapiens cDNA FLJ54349 complete cds, highly similar to Vesicle-fusing ATPase (EC 3.6.4.6). AK294886 - Homo sapiens cDNA FLJ54615 complete cds, highly similar to Vesicle-fusing ATPase (EC 3.6.4.6). AK299786 - Homo sapiens cDNA FLJ54381 complete cds, highly similar to Vesicle-fusing ATPase (EC 3.6.4.6). AK294001 - Homo sapiens cDNA FLJ59316 complete cds, highly similar to Vesicle-fusing ATPase (EC 3.6.4.6). AF102846 - Homo sapiens N-ethylmaleimide-sensitive factor (NSF) mRNA, complete cds. BC030613 - Homo sapiens N-ethylmaleimide-sensitive factor, mRNA (cDNA clone MGC:32977 IMAGE:4812117), complete cds. AK226078 - Homo sapiens mRNA for N-ethylmaleimide-sensitive factor variant, clone: hm00155. AK290204 - Homo sapiens cDNA FLJ75618 complete cds, highly similar to Homo sapiens N-ethylmaleimide-sensitive factor (NSF), mRNA. U03985 - Human N-ethylmaleimide-sensitive factor mRNA, partial cds. AK316057 - Homo sapiens cDNA, FLJ78956 complete cds, highly similar to Vesicle-fusing ATPase (EC 3.6.4.6). AK299031 - Homo sapiens cDNA FLJ58682 complete cds, highly similar to Vesicle-fusing ATPase (EC 3.6.4.6). AB384997 - Synthetic construct DNA, clone: pF1KB4902, Homo sapiens NSF gene for vesicle-fusing ATPase, complete cds, without stop codon, in Flexi system. GQ891288 - Homo sapiens clone HEL-S-11a epididymis secretory sperm binding protein mRNA, complete cds. BC013314 - Homo sapiens N-ethylmaleimide-sensitive factor, mRNA (cDNA clone IMAGE:3939600), partial cds. U80997 - Homo sapiens N-ethylmaleimide-sensitive factor-like protein mRNA, partial cds. JD419276 - Sequence 400300 from Patent EP1572962. JD394070 - Sequence 375094 from Patent EP1572962. JD240235 - Sequence 221259 from Patent EP1572962. JD502891 - Sequence 483915 from Patent EP1572962. JD420002 - Sequence 401026 from Patent EP1572962. JD524375 - Sequence 505399 from Patent EP1572962. JD233877 - Sequence 214901 from Patent EP1572962. JD202508 - Sequence 183532 from Patent EP1572962. JD303461 - Sequence 284485 from Patent EP1572962. JD224071 - Sequence 205095 from Patent EP1572962. JD250713 - Sequence 231737 from Patent EP1572962. JD055480 - Sequence 36504 from Patent EP1572962. JD251210 - Sequence 232234 from Patent EP1572962. JD126267 - Sequence 107291 from Patent EP1572962. JD295011 - Sequence 276035 from Patent EP1572962. JD282175 - Sequence 263199 from Patent EP1572962. JD062439 - Sequence 43463 from Patent EP1572962. JD122700 - Sequence 103724 from Patent EP1572962. AL049276 - Homo sapiens mRNA; cDNA DKFZp564H073 (from clone DKFZp564H073). JD448861 - Sequence 429885 from Patent EP1572962. JD114024 - Sequence 95048 from Patent EP1572962.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_gabaPathway - Gamma-aminobutyric Acid Receptor Life Cycle
Reactome (by CSHL, EBI, and GO)
Protein P46459 (Reactome details) participates in the following event(s):
R-HSA-5694423 NSF and SNAPs bind cis-SNARE complex R-HSA-6809014 cis-Golgi cis-SNARE binds NSF and SNAPs R-HSA-6811425 NSF and SNAPs bind cis-SNARE at the ER membrane R-HSA-6814676 SNAPs and NSF hexamer bind cis-SNARE at the TGN R-HSA-6814684 cis-SNARE binds SNAPs and NSF hexamer at the TGN R-HSA-8847637 NSF and SNAPs bind the cis-SNARE at the Golgi R-HSA-5694425 NSF ATPase activity dissociates cis-SNARE R-HSA-6811422 NSF ATPase activity dissociates cis-SNARE at the ER R-HSA-6814678 ATP hydrolysis by NSF disassembles the cis-SNARE at the TGN R-HSA-6814683 NSF-dependent ATP hydrolysis disassembles the cis-SNARE at the TGN R-HSA-8847638 ATP hydrolysis by NSF disassembles the cis-SNARE at the Golgi membrane R-HSA-416985 Trafficking of GluR2-containing AMPA receptors to synapse R-HSA-204005 COPII-mediated vesicle transport R-HSA-6807878 COPI-mediated anterograde transport R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-HSA-6811440 Retrograde transport at the Trans-Golgi-Network R-HSA-6811438 Intra-Golgi traffic R-HSA-416993 Trafficking of GluR2-containing AMPA receptors R-HSA-199977 ER to Golgi Anterograde Transport R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-399719 Trafficking of AMPA receptors R-HSA-199991 Membrane Trafficking R-HSA-948021 Transport to the Golgi and subsequent modification R-HSA-399721 Glutamate binding, activation of AMPA receptors and synaptic plasticity R-HSA-5653656 Vesicle-mediated transport R-HSA-446203 Asparagine N-linked glycosylation R-HSA-112314 Neurotransmitter receptors and postsynaptic signal transmission R-HSA-597592 Post-translational protein modification R-HSA-112315 Transmission across Chemical Synapses R-HSA-392499 Metabolism of proteins R-HSA-112316 Neuronal System
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
