ID:NUP85_HUMAN DESCRIPTION: RecName: Full=Nuclear pore complex protein Nup85; AltName: Full=85 kDa nucleoporin; AltName: Full=FROUNT; AltName: Full=Nucleoporin Nup75; AltName: Full=Nucleoporin Nup85; AltName: Full=Pericentrin-1; FUNCTION: Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade. SUBUNIT: Component of the nuclear pore complex (NPC). Component of the NPC Nup107-160 subcomplex, consisting of at least NUP107, NUP98/Nup96, NUP160, NUP133, NUP85, NUP37, NUP43 and SEC13. Interacts with NUP160, NUP133 and SEC13. Interacts with NUP37, NUP107 and NUP43. Interacts with CCR2. SUBCELLULAR LOCATION: Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Nucleus membrane. Note=During mitosis, localizes to the kinetochores and spindle poles. Upon CCl2 stimulation translocates from the cytoplasm to the membrane and colocalizes with CCR2 at the front of migrating cells. SIMILARITY: Belongs to the nucleoporin Nup85 family. SEQUENCE CAUTION: Sequence=CAD38749.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9BW27
Front
Top
Side
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.
Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
LF384818 - JP 2014500723-A/192321: Polycomb-Associated Non-Coding RNAs. AK302426 - Homo sapiens cDNA FLJ59007 complete cds, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA. JD076891 - Sequence 57915 from Patent EP1572962. AF514995 - Homo sapiens nucleoporin Nup75 mRNA, complete cds. BC000697 - Homo sapiens nucleoporin 85kDa, mRNA (cDNA clone MGC:3092 IMAGE:3349383), complete cds. JD472040 - Sequence 453064 from Patent EP1572962. AK297574 - Homo sapiens cDNA FLJ53138 complete cds, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA. AK295694 - Homo sapiens cDNA FLJ54688 complete cds, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA. AK298519 - Homo sapiens cDNA FLJ59373 complete cds, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA. AK022611 - Homo sapiens cDNA FLJ12549 fis, clone NT2RM4000689. AL833893 - Homo sapiens mRNA; cDNA DKFZp434I1130 (from clone DKFZp434I1130). CU674344 - Synthetic construct Homo sapiens gateway clone IMAGE:100017759 5' read NUP85 mRNA. KJ899587 - Synthetic construct Homo sapiens clone ccsbBroadEn_08981 NUP85 gene, encodes complete protein. AF498261 - Homo sapiens frount protein mRNA, complete cds. LF328390 - JP 2014500723-A/135893: Polycomb-Associated Non-Coding RNAs. LF328391 - JP 2014500723-A/135894: Polycomb-Associated Non-Coding RNAs. LF328392 - JP 2014500723-A/135895: Polycomb-Associated Non-Coding RNAs. AK124501 - Homo sapiens cDNA FLJ42510 fis, clone BRACE2044473, highly similar to Homo sapiens pericentrin 1 (PCNT1), mRNA. HZ473963 - WO 2016002844-A/77: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL. HZ473964 - WO 2016002844-A/78: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL. HZ473966 - WO 2016002844-A/80: ANTI-INVASIVE/ANTI-METASTATIC DRUG FOR PANCREATIC CANCER CELL. MA620395 - JP 2018138019-A/192321: Polycomb-Associated Non-Coding RNAs. MA563967 - JP 2018138019-A/135893: Polycomb-Associated Non-Coding RNAs. MA563968 - JP 2018138019-A/135894: Polycomb-Associated Non-Coding RNAs. MA563969 - JP 2018138019-A/135895: Polycomb-Associated Non-Coding RNAs.
Biochemical and Signaling Pathways
BioCarta from NCI Cancer Genome Anatomy Project h_akapCentrosomePathway - Protein Kinase A at the Centrosome
Reactome (by CSHL, EBI, and GO)
Protein Q9BW27 (Reactome details) participates in the following event(s):
R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-165043 Rev multimer-bound HIV mRNA:Crm1:Ran:GTP complex associates with the NPC R-HSA-180622 Vpr binds nucleoporins R-HSA-180710 Rev:importin beta:B23 recruited to the nuclear pore R-HSA-2990882 CDK1 phosphorylates NUP98 R-HSA-75098 mRNP complex dissociates from cytosolic face of NPC R-HSA-165047 Translocation of nuclear RNA transport complex to cytoplasm R-HSA-180732 Translocation of Rev:importin-beta:B23 to the nucleus R-HSA-2990880 NEK6/NEK7 phosphorylates NUP98 R-HSA-170796 NPC transports GCK1:GKRP from cytosol to nucleoplasm R-HSA-5578744 Importin-8 imports AGO2:miRNA into the nucleus R-HSA-6783483 tRNA:XPOT:RAN:GTP translocates from the nucleus to the cytosol R-HSA-3000348 RANBP2 SUMOylates SP100 with SUMO2 R-HSA-3000399 RANBP2 SUMOylates SP100 with SUMO1 R-HSA-3000411 RANBP2 SUMOylates PML with SUMO2 R-HSA-4570493 RANBP2 (NUP358) SUMOylates HNRNPC with SUMO1 R-HSA-4615872 RANBP2 SUMOylates HDAC4 with SUMO1 R-HSA-4615987 RANBP2 SUMOylates HDAC4 with SUMO2,3 R-HSA-5228508 RANBP2 SUMOylates PML with SUMO1 R-HSA-5228525 RANBP2 SUMOylates TOP2A with SUMO1 R-HSA-192627 Viral mRNA Export R-HSA-192925 Export of Spliced Viral mRNA R-HSA-5252041 NPC transports Hikeshi:HSP70s:ATP from cytosol to nucleoplasm R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-165054 Rev-mediated nuclear export of HIV RNA R-HSA-180910 Vpr-mediated nuclear import of PICs R-HSA-180746 Nuclear import of Rev protein R-HSA-3301854 Nuclear Pore Complex (NPC) Disassembly R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-162599 Late Phase of HIV Life Cycle R-HSA-177243 Interactions of Rev with host cellular proteins R-HSA-176033 Interactions of Vpr with host cellular proteins R-HSA-2980766 Nuclear Envelope Breakdown R-HSA-170822 Regulation of Glucokinase by Glucokinase Regulatory Protein R-HSA-168276 NS1 Mediated Effects on Host Pathways R-HSA-1169408 ISG15 antiviral mechanism R-HSA-5578749 Transcriptional regulation by small RNAs R-HSA-6784531 tRNA processing in the nucleus R-HSA-3108214 SUMOylation of DNA damage response and repair proteins R-HSA-4570464 SUMOylation of RNA binding proteins R-HSA-4551638 SUMOylation of chromatin organization proteins R-HSA-4615885 SUMOylation of DNA replication proteins R-HSA-168325 Viral Messenger RNA Synthesis R-HSA-3371453 Regulation of HSF1-mediated heat shock response R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-162587 HIV Life Cycle R-HSA-162909 Host Interactions of HIV factors R-HSA-68875 Mitotic Prophase R-HSA-70171 Glycolysis R-HSA-168253 Host Interactions with Influenza Factors R-HSA-1169410 Antiviral mechanism by IFN-stimulated genes R-HSA-211000 Gene Silencing by RNA R-HSA-72306 tRNA processing R-HSA-3108232 SUMO E3 ligases SUMOylate target proteins R-HSA-168273 Influenza Viral RNA Transcription and Replication R-HSA-3371556 Cellular response to heat stress R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-8953854 Metabolism of RNA R-HSA-162906 HIV Infection R-HSA-70326 Glucose metabolism R-HSA-168254 Influenza Infection R-HSA-913531 Interferon Signaling R-HSA-74160 Gene expression (Transcription) R-HSA-2990846 SUMOylation R-HSA-168255 Influenza Life Cycle R-HSA-2262752 Cellular responses to stress R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-5663205 Infectious disease R-HSA-71387 Metabolism of carbohydrates R-HSA-1280215 Cytokine Signaling in Immune system R-HSA-597592 Post-translational protein modification R-HSA-8953897 Cellular responses to external stimuli R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction R-HSA-1643685 Disease R-HSA-1430728 Metabolism R-HSA-168256 Immune System R-HSA-392499 Metabolism of proteins
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
