Human Gene OLA1 (ENST00000284719.8_7) from GENCODE V47lift37
  Description: Obg like ATPase 1, transcript variant 1 (from RefSeq NM_013341.5)
Gencode Transcript: ENST00000284719.8_7
Gencode Gene: ENSG00000138430.16_12
Transcript (Including UTRs)
   Position: hg19 chr2:174,937,175-175,113,260 Size: 176,086 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr2:174,940,154-175,111,543 Size: 171,390 Coding Exon Count: 10 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:174,937,175-175,113,260)mRNA (may differ from genome)Protein (396 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: OLA1_HUMAN
DESCRIPTION: RecName: Full=Obg-like ATPase 1; EC=3.6.3.-; AltName: Full=DNA damage-regulated overexpressed in cancer 45; Short=DOC45; AltName: Full=GTP-binding protein 9;
FUNCTION: Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.
CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.
SUBUNIT: Monomer.
INTERACTION: P04591:gag (xeno); NbExp=4; IntAct=EBI-766468, EBI-6179719;
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Nucleus, nucleolus. Note=Predominantly cytoplasmic, shuttles between the nucleus and the cytoplasm.
TISSUE SPECIFICITY: Expressed in all tissues tested but its expression is more abundant in testis, liver, lung, and brain. Overexpressed in several malignancies, including cancers of the colon, rectum, ovary, lung, stomach, and uterus.
INDUCTION: Strongly down-regulated by DNA damage-inducing agents.
SIMILARITY: Belongs to the GTP1/OBG family.
SIMILARITY: Contains 1 G (guanine nucleotide-binding) domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.28 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 461.07 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -23.6081-0.291 Picture PostScript Text
3' UTR -752.302979-0.253 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012675 - Beta-grasp_dom
IPR004396 - CHP00092
IPR013029 - DUF933
IPR006073 - GTP_binding_domain
IPR012676 - TGS-like
IPR023192 - TGS-like_dom

Pfam Domains:
PF01926 - 50S ribosome-binding GTPase
PF02421 - Ferrous iron transport protein B
PF06071 - Protein of unknown function (DUF933)

SCOP Domains:
52540 - P-loop containing nucleoside triphosphate hydrolases
81271 - TGS-like

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2OHF - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9NTK5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0005525 GTP binding
GO:0016787 hydrolase activity
GO:0016887 ATPase activity
GO:0045296 cadherin binding
GO:0046872 metal ion binding

Biological Process:
GO:0002576 platelet degranulation
GO:0046034 ATP metabolic process

Cellular Component:
GO:0005576 extracellular region
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0005737 cytoplasm
GO:0005813 centrosome
GO:0005829 cytosol
GO:0016020 membrane
GO:0031093 platelet alpha granule lumen
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC068447 - Homo sapiens cDNA clone IMAGE:5276010, containing frame-shift errors.
BC091522 - Homo sapiens Obg-like ATPase 1, mRNA (cDNA clone MGC:111587 IMAGE:30513213), complete cds.
AF134478 - Homo sapiens GTP-binding protein mRNA, complete cds.
AF078868 - Homo sapiens homologous yeast-44.2 protein mRNA, complete cds.
AF078859 - Homo sapiens PTD004 mRNA, complete cds.
AK225138 - Homo sapiens mRNA for GTP-binding protein PTD004 isoform 1 variant, clone: CBL08160.
BC012842 - Homo sapiens Obg-like ATPase 1, mRNA (cDNA clone MGC:9241 IMAGE:3861915), complete cds.
AK074710 - Homo sapiens cDNA FLJ90229 fis, clone NT2RM2000306, moderately similar to PUTATIVE GTP-BINDING PROTEIN W08E3.3.
AK027523 - Homo sapiens cDNA FLJ14617 fis, clone NT2RP1001410, moderately similar to PUTATIVE GTP-BINDING PROTEIN W08E3.3.
AL833441 - Homo sapiens mRNA; cDNA DKFZp313H1942 (from clone DKFZp313H1942).
BC013925 - Homo sapiens Obg-like ATPase 1, mRNA (cDNA clone MGC:24203 IMAGE:3685016), complete cds.
DQ250006 - Homo sapiens DNA damage-regulated overexpressed in cancer 45 protein mRNA, complete cds.
AK225112 - Homo sapiens mRNA for GTP-binding protein PTD004 isoform 1 variant, clone: CAS10854.
BC029376 - Homo sapiens Obg-like ATPase 1, mRNA (cDNA clone MGC:32564 IMAGE:4658276), complete cds.
AK296339 - Homo sapiens cDNA FLJ58890 complete cds, highly similar to GTP-binding protein PTD004.
AL136546 - Homo sapiens mRNA; cDNA DKFZp761C10121 (from clone DKFZp761C10121); complete cds.
AF116703 - Homo sapiens PRO2455 mRNA, complete cds.
KJ902510 - Synthetic construct Homo sapiens clone ccsbBroadEn_11904 OLA1 gene, encodes complete protein.
KJ898727 - Synthetic construct Homo sapiens clone ccsbBroadEn_08121 OLA1 gene, encodes complete protein.
AB463153 - Synthetic construct DNA, clone: pF1KB8671, Homo sapiens OLA1 gene for Obg-like ATPase 1, without stop codon, in Flexi system.
AM393454 - Synthetic construct Homo sapiens clone IMAGE:100001836 for hypothetical protein (PTD004 gene).
CU680408 - Synthetic construct Homo sapiens gateway clone IMAGE:100017177 5' read OLA1 mRNA.
JD486729 - Sequence 467753 from Patent EP1572962.
JD329910 - Sequence 310934 from Patent EP1572962.
JD237541 - Sequence 218565 from Patent EP1572962.
JD415146 - Sequence 396170 from Patent EP1572962.
JD454471 - Sequence 435495 from Patent EP1572962.
JD510237 - Sequence 491261 from Patent EP1572962.
JD379509 - Sequence 360533 from Patent EP1572962.
JD037582 - Sequence 18606 from Patent EP1572962.
JD341478 - Sequence 322502 from Patent EP1572962.
JD194379 - Sequence 175403 from Patent EP1572962.
JD071146 - Sequence 52170 from Patent EP1572962.
JD443762 - Sequence 424786 from Patent EP1572962.
JD532489 - Sequence 513513 from Patent EP1572962.
JD431123 - Sequence 412147 from Patent EP1572962.
JD522651 - Sequence 503675 from Patent EP1572962.
JD074261 - Sequence 55285 from Patent EP1572962.
JD083203 - Sequence 64227 from Patent EP1572962.
JD229303 - Sequence 210327 from Patent EP1572962.
JD564477 - Sequence 545501 from Patent EP1572962.
JD341067 - Sequence 322091 from Patent EP1572962.
JD446764 - Sequence 427788 from Patent EP1572962.
JD139156 - Sequence 120180 from Patent EP1572962.
JD358813 - Sequence 339837 from Patent EP1572962.
JD184431 - Sequence 165455 from Patent EP1572962.
JD037224 - Sequence 18248 from Patent EP1572962.
JD063436 - Sequence 44460 from Patent EP1572962.
JD403579 - Sequence 384603 from Patent EP1572962.
JD239716 - Sequence 220740 from Patent EP1572962.
JD227129 - Sequence 208153 from Patent EP1572962.
JD416876 - Sequence 397900 from Patent EP1572962.
JD136660 - Sequence 117684 from Patent EP1572962.
JD070315 - Sequence 51339 from Patent EP1572962.
JD210916 - Sequence 191940 from Patent EP1572962.
JD303107 - Sequence 284131 from Patent EP1572962.
JD520960 - Sequence 501984 from Patent EP1572962.
JD287577 - Sequence 268601 from Patent EP1572962.
JD045058 - Sequence 26082 from Patent EP1572962.
JD316696 - Sequence 297720 from Patent EP1572962.
JD353965 - Sequence 334989 from Patent EP1572962.
JD188786 - Sequence 169810 from Patent EP1572962.
JD344545 - Sequence 325569 from Patent EP1572962.
LF361046 - JP 2014500723-A/168549: Polycomb-Associated Non-Coding RNAs.
DL492458 - Novel nucleic acids.
DL490935 - Novel nucleic acids.
MA596623 - JP 2018138019-A/168549: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9NTK5 (Reactome details) participates in the following event(s):

R-HSA-481007 Exocytosis of platelet alpha granule contents
R-HSA-114608 Platelet degranulation
R-HSA-76005 Response to elevated platelet cytosolic Ca2+
R-HSA-76002 Platelet activation, signaling and aggregation
R-HSA-109582 Hemostasis

-  Other Names for This Gene
  Alternate Gene Symbols: D7EHM2, ENST00000284719.1, ENST00000284719.2, ENST00000284719.3, ENST00000284719.4, ENST00000284719.5, ENST00000284719.6, ENST00000284719.7, GTPBP9, NM_013341, OLA1 , OLA1_HUMAN, PRO2455, PTD004, Q5BJD7, Q8NCI8, Q96CU1, Q96SV2, Q9NTK5, Q9P1D3, Q9UNY9, Q9Y6G4, uc317kfv.1, uc317kfv.2
UCSC ID: ENST00000284719.8_7
RefSeq Accession: NM_013341.5
Protein: Q9NTK5 (aka OLA1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.