Human Gene PARP12 (ENST00000263549.8_6) from GENCODE V47lift37
  Description: poly(ADP-ribose) polymerase family member 12, transcript variant 1 (from RefSeq NM_022750.4)
Gencode Transcript: ENST00000263549.8_6
Gencode Gene: ENSG00000059378.13_10
Transcript (Including UTRs)
   Position: hg19 chr7:139,723,549-139,762,751 Size: 39,203 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr7:139,724,360-139,762,647 Size: 38,288 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:139,723,549-139,762,751)mRNA (may differ from genome)Protein (701 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PAR12_HUMAN
DESCRIPTION: RecName: Full=Poly [ADP-ribose] polymerase 12; Short=PARP-12; EC=2.4.2.30; AltName: Full=ADP-ribosyltransferase diphtheria toxin-like 12; Short=ARTD12; AltName: Full=Zinc finger CCCH domain-containing protein 1;
CATALYTIC ACTIVITY: NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
SUBCELLULAR LOCATION: Nucleus (Probable).
SIMILARITY: Contains 4 C3H1-type zinc fingers.
SIMILARITY: Contains 1 PARP catalytic domain.
SIMILARITY: Contains 2 WWE domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 18.25 RPKM in Small Intestine - Terminal Ileum
Total median expression: 303.57 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -41.10104-0.395 Picture PostScript Text
3' UTR -250.30811-0.309 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012317 - Poly(ADP-ribose)pol_cat_dom
IPR004170 - WWE-dom
IPR000571 - Znf_CCCH

Pfam Domains:
PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar)
PF00644 - Poly(ADP-ribose) polymerase catalytic domain
PF02825 - WWE domain
PF14608 - RNA-binding, Nab2-type zinc finger

SCOP Domains:
56399 - ADP-ribosylation
117839 - WWE domain
90229 - CCCH zinc finger

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2PQF - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9H0J9
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003723 RNA binding
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Cellular Component:
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AF173884 - Homo sapiens MSTP109 (MST109) mRNA, complete cds.
AK026346 - Homo sapiens cDNA: FLJ22693 fis, clone HSI11445.
AL137255 - Homo sapiens mRNA; cDNA DKFZp434B1813 (from clone DKFZp434B1813); partial cds.
BC044660 - Homo sapiens poly (ADP-ribose) polymerase family, member 12, mRNA (cDNA clone IMAGE:4428430), containing frame-shift errors.
AL136766 - Homo sapiens mRNA; cDNA DKFZp434A0516 (from clone DKFZp434A0516); complete cds.
BC081541 - Homo sapiens poly (ADP-ribose) polymerase family, member 12, mRNA (cDNA clone MGC:71625 IMAGE:5296560), complete cds.
JD339152 - Sequence 320176 from Patent EP1572962.
JD345754 - Sequence 326778 from Patent EP1572962.
JD522623 - Sequence 503647 from Patent EP1572962.
JD040251 - Sequence 21275 from Patent EP1572962.
JD092945 - Sequence 73969 from Patent EP1572962.
JD276509 - Sequence 257533 from Patent EP1572962.
JD364019 - Sequence 345043 from Patent EP1572962.
JD098718 - Sequence 79742 from Patent EP1572962.
JD057861 - Sequence 38885 from Patent EP1572962.
JD467898 - Sequence 448922 from Patent EP1572962.
JD566421 - Sequence 547445 from Patent EP1572962.
JD566426 - Sequence 547450 from Patent EP1572962.
JD246530 - Sequence 227554 from Patent EP1572962.
JD118995 - Sequence 100019 from Patent EP1572962.
JD518495 - Sequence 499519 from Patent EP1572962.
JD500172 - Sequence 481196 from Patent EP1572962.
KJ899471 - Synthetic construct Homo sapiens clone ccsbBroadEn_08865 PARP12 gene, encodes complete protein.
JD209716 - Sequence 190740 from Patent EP1572962.
JD517074 - Sequence 498098 from Patent EP1572962.
JD392874 - Sequence 373898 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000263549.1, ENST00000263549.2, ENST00000263549.3, ENST00000263549.4, ENST00000263549.5, ENST00000263549.6, ENST00000263549.7, NM_022750, PAR12_HUMAN, PARP12 , Q9H0J9, Q9H610, Q9NP36, Q9NTI3, uc317hkh.1, uc317hkh.2, ZC3HDC1
UCSC ID: ENST00000263549.8_6
RefSeq Accession: NM_022750.4
Protein: Q9H0J9 (aka PAR12_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.