Human Gene PARP8 (ENST00000281631.10_4) from GENCODE V47lift37
  Description: poly(ADP-ribose) polymerase family member 8, transcript variant 2 (from RefSeq NM_024615.4)
Gencode Transcript: ENST00000281631.10_4
Gencode Gene: ENSG00000151883.20_9
Transcript (Including UTRs)
   Position: hg19 chr5:49,962,471-50,142,353 Size: 179,883 Total Exon Count: 26 Strand: +
Coding Region
   Position: hg19 chr5:49,962,930-50,137,902 Size: 174,973 Coding Exon Count: 26 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:49,962,471-50,142,353)mRNA (may differ from genome)Protein (854 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PARP8_HUMAN
DESCRIPTION: RecName: Full=Poly [ADP-ribose] polymerase 8; Short=PARP-8; EC=2.4.2.30; AltName: Full=ADP-ribosyltransferase diphtheria toxin-like 16; Short=ARTD16;
CATALYTIC ACTIVITY: NAD(+) + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Contains 1 PARP catalytic domain.
SEQUENCE CAUTION: Sequence=AAI05790.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 13.16 RPKM in Spleen
Total median expression: 172.97 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -198.70459-0.433 Picture PostScript Text
3' UTR -958.504451-0.215 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR012317 - Poly(ADP-ribose)pol_cat_dom

Pfam Domains:
PF00644 - Poly(ADP-ribose) polymerase catalytic domain

SCOP Domains:
56399 - ADP-ribosylation

ModBase Predicted Comparative 3D Structure on Q8N3A8
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003950 NAD+ ADP-ribosyltransferase activity
GO:0016740 transferase activity
GO:0016757 transferase activity, transferring glycosyl groups

Cellular Component:
GO:0005622 intracellular


-  Descriptions from all associated GenBank mRNAs
  BC105789 - Homo sapiens poly (ADP-ribose) polymerase family, member 8, mRNA (cDNA clone IMAGE:4293356), partial cds.
AK314466 - Homo sapiens cDNA, FLJ95269, highly similar to Homo sapiens poly (ADP-ribose) polymerase family, member 8 (PARP8), mRNA.
AK298680 - Homo sapiens cDNA FLJ57558 complete cds, highly similar to Homo sapiens poly (ADP-ribose) polymerase family, member 8 (PARP8), mRNA.
AK289369 - Homo sapiens cDNA FLJ78221 complete cds, highly similar to Homo sapiens poly (ADP-ribose) polymerase family, member 8 (PARP8), mRNA.
BC075801 - Homo sapiens poly (ADP-ribose) polymerase family, member 8, mRNA (cDNA clone MGC:87748 IMAGE:5226828), complete cds.
AL834477 - Homo sapiens mRNA; cDNA DKFZp762K2011 (from clone DKFZp762K2011).
AB463183 - Synthetic construct DNA, clone: pF1KB8577, Homo sapiens PARP8 gene for poly (ADP-ribose) polymerase family, member 8, without stop codon, in Flexi system.
AM393004 - Synthetic construct Homo sapiens clone IMAGE:100001879 for hypothetical protein (PARP8 gene).
BC037386 - Homo sapiens poly (ADP-ribose) polymerase family, member 8, mRNA (cDNA clone MGC:42864 IMAGE:4830820), complete cds.
AK024961 - Homo sapiens cDNA: FLJ21308 fis, clone COL02131.
AK225188 - Homo sapiens mRNA for poly (ADP-ribose) polymerase family, member 8 variant, clone: COL02131.
BC058033 - Homo sapiens cDNA clone IMAGE:6108023, with apparent retained intron.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8N3A8 (Reactome details) participates in the following event(s):

R-HSA-197264 Nicotinamide salvaging
R-HSA-196807 Nicotinate metabolism
R-HSA-196849 Metabolism of water-soluble vitamins and cofactors
R-HSA-196854 Metabolism of vitamins and cofactors
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000281631.1, ENST00000281631.2, ENST00000281631.3, ENST00000281631.4, ENST00000281631.5, ENST00000281631.6, ENST00000281631.7, ENST00000281631.8, ENST00000281631.9, NM_024615, PARP8 , PARP8_HUMAN, Q3KRB7, Q6DHZ1, Q8N3A8, Q9H754, uc317jwz.1, uc317jwz.2
UCSC ID: ENST00000281631.10_4
RefSeq Accession: NM_024615.4
Protein: Q8N3A8 (aka PARP8_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.