Human Gene PCBP1 (ENST00000303577.7_4) from GENCODE V47lift37
  Description: poly(rC) binding protein 1 (from RefSeq NM_006196.4)
Gencode Transcript: ENST00000303577.7_4
Gencode Gene: ENSG00000169564.7_8
Transcript (Including UTRs)
   Position: hg19 chr2:70,314,609-70,316,335 Size: 1,727 Total Exon Count: 1 Strand: +
Coding Region
   Position: hg19 chr2:70,314,876-70,315,946 Size: 1,071 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:70,314,609-70,316,335)mRNA (may differ from genome)Protein (356 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIOMIMPubMedUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: Q53SS8_HUMAN
DESCRIPTION: SubName: Full=Poly(RC) binding protein 1; SubName: Full=Putative uncharacterized protein PCBP1; SubName: Full=cDNA, FLJ94964, highly similar to Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA;
SIMILARITY: Contains 3 KH domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 345.97 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 8110.92 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -71.60267-0.268 Picture PostScript Text
3' UTR -71.70389-0.184 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR004087 - KH_dom
IPR004088 - KH_dom_type_1

Pfam Domains:
PF00013 - KH domain

SCOP Domains:
54791 - Eukaryotic type KH-domain (KH-domain type I)
54814 - Prokaryotic type KH domain (KH-domain type II)

ModBase Predicted Comparative 3D Structure on Q53SS8
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003697 single-stranded DNA binding
GO:0003723 RNA binding
GO:0098847 sequence-specific single stranded DNA binding

Biological Process:
GO:0045944 positive regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016607 nuclear speck
GO:0036464 cytoplasmic ribonucleoprotein granule


-  Descriptions from all associated GenBank mRNAs
  JD191900 - Sequence 172924 from Patent EP1572962.
U24223 - Human alpha-CP1 mRNA, complete cds.
AK314225 - Homo sapiens cDNA, FLJ94964, highly similar to Homo sapiens poly(rC) binding protein 1 (PCBP1), mRNA.
JD174173 - Sequence 155197 from Patent EP1572962.
BC039742 - Homo sapiens poly(rC) binding protein 1, mRNA (cDNA clone MGC:47561 IMAGE:6061411), complete cds.
FJ224329 - Homo sapiens epididymis secretory protein Li 85 (HEL-S-85) mRNA, complete cds.
Z29505 - H.sapiens mRNA for nucleic acid binding protein sub2.3.
X78137 - H.sapiens hnRNP-E1 mRNA.
JD415918 - Sequence 396942 from Patent EP1572962.
AB464656 - Synthetic construct DNA, clone: pF1KB9840, Homo sapiens PCBP1 gene for poly(rC) binding protein 1, without stop codon, in Flexi system.
CU691762 - Synthetic construct Homo sapiens gateway clone IMAGE:100020863 5' read PCBP1 mRNA.
HQ447984 - Synthetic construct Homo sapiens clone IMAGE:100071351; CCSB010250_03 poly(rC) binding protein 1 (PCBP1) gene, encodes complete protein.
KJ891755 - Synthetic construct Homo sapiens clone ccsbBroadEn_01149 PCBP1 gene, encodes complete protein.
AK130439 - Homo sapiens cDNA FLJ26929 fis, clone RCT05551, highly similar to Poly(rC)-binding protein 1.
BC013020 - Homo sapiens cDNA clone IMAGE:3349652, **** WARNING: chimeric clone ****.
JD313112 - Sequence 294136 from Patent EP1572962.
JD082575 - Sequence 63599 from Patent EP1572962.
JD515867 - Sequence 496891 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000303577.1, ENST00000303577.2, ENST00000303577.3, ENST00000303577.4, ENST00000303577.5, ENST00000303577.6, hCG_1776997 , HEL-S-85 , NM_006196, PCBP1 , Q53SS8, Q53SS8_HUMAN, uc317ngl.1, uc317ngl.2
UCSC ID: ENST00000303577.7_4
RefSeq Accession: NM_006196.4
Protein: Q53SS8

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.